Enzyme-linked immunosorbent assay and immunochromatographic strip for rapid detection of atrazine in water samples

Yu, Na; Sheng, Wei; Yuan, Meng; Li, Lingling; Liu, Bing; Zhang, Yan; Wang, Shuo

doi:10.1007/s00604-012-0772-y

Cited by 20 publications

(4 citation statements)

References 33 publications

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“…The haptens were designed and synthesized in our previous work. 13 Both haptens were applied to couple with BSA and OVA to prepare the immunogens and coating antigens, respectively. All the conjugates were prepared with the activated N-hydroxysuccinimide (NHS) ester method.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Computer-Aided Molecular Modeling Study on Antibody Recognition of Small Molecules: An Immunoassay for Triazine Herbicides

Yuan

Liu

et al. 2012

J. Agric. Food Chem.

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Most immunoassays for determination of small molecules are still designed on the basis of the "trial and error" method, due to the lack of understanding of antibody recognition. In the present study, we developed a heterologous indirect competitive enzyme-linked immunosorbent assay for determination of triazine herbicides, with limits of detection for 11 triazines ranging from 0.05 to 29.4 μg/L. Mechanisms of the antigen-antibody interaction were studied by computer-aided molecular modeling (CAMM)-based quantitative structure-activity relationship analyses. Co-effects of the analytes' substructural hydrophobic, electrostatic, and steric fields on antibody recognition were further revealed. Hydrophobicity of the antigens was demonstrated to have the most important impact. Even less exposed substituents provided hydrophobic force to the antigen-antibody interaction. Dislocated orientation of analyte functional groups could lead to steric hindrance and hydrophobic misleading of antibody recognition. This may happen even when the antigens contained the same substituent as the hapten. Frontier orbital energies also affect the reaction significantly. This study highlights of the power of CAMM-based analyses, providing insights into antibody recognition of small molecules.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The haptens were designed and synthesized in our previous work . Both haptens were applied to couple with BSA and OVA to prepare the immunogens and coating antigens, respectively.…”

Section: Methodsmentioning

confidence: 99%

Computer-Aided Molecular Modeling Study on Antibody Recognition of Small Molecules: An Immunoassay for Triazine Herbicides

Yuan

Liu

et al. 2012

J. Agric. Food Chem.

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“…A direct competitive enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-based immunochromatographic (ICG) strip was developed for rapid analysis of ATZ in water samples. Both the ELISA and ICG strip assay presented high specificity, sensitivity, and fast speed with its LOD of 0.01 ng/mL and 2 ng/mL, respectively [24]. Cao et al developed an UPLC-TOF-MS/MS method to investigate the metabolic pathway of ATZ by a high-efficient bacterial strain, Arthrobacter sp.…”

Section: Introductionmentioning

confidence: 99%

Residual Characteristics of Atrazine and Its Metabolites in the Liaoning Province of China

Wang¹,

Wang²,

Li³

et al. 2022

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The simultaneous determination of atrazine residue and its metabolites was developed using liquid chromatography-tandem mass spectrometry in Liaoning Province, China. To ensure agricultural production and environmental safety, their contamination level was assessed. A total of 2142 samples were collected between 2014 and 2020, including 1213 soil samples, 190 surface water samples, and 739 groundwater samples. The overall pollution level and detectable level of the herbicides in Liaoning Province was found to be the highest in soil followed by surface water and groundwater. The residual level of the analytes in the collected samples decreased in the following order: atrazine > hydroxyatrazine > desethylatrazine > desisopropylatrazine. From 2014 to 2020, atrazine was detected in soil and surface water, whereas hydroxyatrazine was found in soil without the selected analytes detected in groundwater. The pollution of atrazine in soil was higher than that of hydroxyatrazine, desethylatrazine, and desisopropylatrazine. To maintain sustainable agricultural development, it is critical to pay attention to the residual determination of atrazine in the environment.

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“…38,39 And the synthetic colloidal golds were uniformly spherical and presented high monodispersity with respective average sizes of approximately 20 nm.…”

Section: Preparation Of Colloidal Gold and Gold Labeled Mcabmentioning

confidence: 99%

Quantum dot based multiplex fluorescence quenching immune chromatographic strips for the simultaneous determination of sulfonamide and fluoroquinolone residues in chicken samples

Sheng

et al. 2017

RSC Adv.

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A novel multiplex fluorescence quenching immune chromatographic strip (FQICS), using quantum dots (QDs) as a fluorescence donor, was proposed to screen sulfonamide (SA) and fluoroquinolone (FQ) residues in chicken samples, simultaneously. The principle of the proposed FQICS is on the basis of the fluorescence quenching effect, and colloidal gold was used as the fluorescence quencher. The limit of detection (LOD) in standard solution was 3 ng mL À1 and 0.6 ng mL À1 for sulfaquinoxaline (SQX) and norfloxacin (NOR), respectively, through visual detection using UV. For specificity analysis, results obtained indicated that this established method could be applied in broad-spectrum detection of SAs and FQs. Then the proposed FQICS was successfully applied in semiquantitative analysis of SQX (30 mg kg À1) and NOR (6 mg kg À1) in spiked chicken samples. The results obtained using the strips showed great agreement with those obtained using an ELISA kit, which indicates the good accuracy of the strips. As a conclusion, the proposed FQICS with a signal "turn-on" mode provides a suitable immunoassay for rapid, sensitive and simultaneous screening of SAs and FQs in chicken samples on-site.

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Enzyme-linked immunosorbent assay and immunochromatographic strip for rapid detection of atrazine in water samples

Cited by 20 publications

References 33 publications

Computer-Aided Molecular Modeling Study on Antibody Recognition of Small Molecules: An Immunoassay for Triazine Herbicides

Computer-Aided Molecular Modeling Study on Antibody Recognition of Small Molecules: An Immunoassay for Triazine Herbicides

Residual Characteristics of Atrazine and Its Metabolites in the Liaoning Province of China

Quantum dot based multiplex fluorescence quenching immune chromatographic strips for the simultaneous determination of sulfonamide and fluoroquinolone residues in chicken samples

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