Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Angiostrongylus cantonensis

The role of corticosteroids in the treatment of eosinophilic meningitis has not been definitely established. Patients given a 2-week course of prednisolone (treatment group), 60 mg/day, were compared with those given placebo (control group) in a randomized, double-blind trial. Fifty-five patients were enrolled in each group. There were significant differences between the treatment and control groups, with regard to the number of patients who still had headache after 14 days (5 vs. 25, respectively; P=.00004), the median length of time until complete disappearance of headache (5 vs. 13 days, respectively; P=.00000), and the number of patients who had repeat lumbar puncture (7 vs. 22, respectively; P=.002). Serious side effects were not detected. These results indicate that a 2-week course of prednisolone was beneficial in relieving headache in patients with eosinophilic meningitis.

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“…A number of serological tests have been used to support the diagnosis of angiostrongyliasis. ELISA appears to show the most promise [5,6].…”

Section: Discussionmentioning

confidence: 99%

Corticosteroid Treatment of Eosinophilic Meningitis

Chotmongkol

Sawanyawisuth

Thavornpitak

2000

Clinical Infectious Diseases

134

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“…Proven cases of human infection showing worms are however rare (Punyagupta, 1979) and the ELISA utilizing either crude or partially purified Parastrongylus antigens is currently used as a reliable method for immunodiagnosis of the infection (Welch et al, 1980;Chen, 1986;Yen and Chen, 1991) . Although ELISA is sensitive enough for detecting serum antibodies to P. cantonensis, it is expensive, laborious and limiting with respect to the length of time to get results.…”

Section: Discussionmentioning

confidence: 99%

Detection of antibodies to Parastrongylus cantonensis in human sera by gelatin particle indirect agglutination test.

Eamsobhana

Watthanakulpanich

Punthuprapasa

et al. 1999

Jap. J. Trop. Med. Hyg.

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Abstract:A newly developed agglutination test using gelatin particles as an antigen carrier (GPAT) was compared with a conventional enzyme-linked immunosorbent assay (ELISA) for the detection of Parastrongylus cantonensis antibodies in sera from patients. A total of 70 sera was used in the study. Of these, 10 each were from patients with parastrongyliasis, gnathostomiasis, paragonimiasis, cysticercosis, toxocariasis, filariasis and malaria.The control group consisted of 50 serum samples from normal healthy individuals. The mean reciprocal titer of the parastrongyliasis patients group was significantly higher than that of the normal group as well as those of other parasitic infections. The sensitivity and specificity of the GPAT were 100% and 92.4%, respectively. The results of GPAT in detecting P. cantonensis antibodies appeared to be closely correlated with those obtained with ELISA. The GPAT, however, is more easy, rapid and cheap; it may also be a test of choice for routine immunodiagnosis of human parastrongyliasis.

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“…As there is a low recovery rate of worms in the CSF, development of a new diagnostic technique is emergent and necessary. Nowadays, several immunological methods of angiostrongyliasis diagnosis have been carried out (Chen 1986;Chye et al 2000;Intapan et al 2003;Kittimongkolma et al 2007), but the sensitivity and specificity of tests vary and need improvement.…”

Section: Introductionmentioning

confidence: 98%

Cloning and characterization of a novel gene encoding 16 kDa protein (Ac16) from Angiostrongylus cantonensis

Lv²,

Wei³

et al. 2011

Parasitol Res

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Angiostrongylus cantonensis is the most common infectious cause of eosinophilic meningitis or meningoencephalitis. A novel gene (AC16) was isolated from a cDNA library of A. cantonensis fourth-stage larvae. The putative 16-kDa protein has 149 amino acids and is homologous to an immunodominant hypodermal antigen (IHA16) from Ancylostoma caninum (identities = 57%). In this paper, we cloned the gene and purified the recombinant Ac16 (rAC16) protein. Real-time quantitative PCR revealed that Ac16 was expressed significantly higher in the fourth-stage larvae and adult worms derived from rats than that in the fourth-stage larvae derived from mice. Moreover, sera from rat (permissive host) infected with A. cantonensis detected Ac16 by Western blot, while sera from infected mouse (non-permissive host) could not. The results implied that Ac16 was related to the parasitic adaptation of A. cantonensis in different hosts and non-permissive host mouse had no circulating antibody to the antigen Ac16 from A. cantonensis and thus might contribute to understanding the mechanism of parasite immune evasion. Furthermore, we evaluated the ability of Ac16 antibody diagnosing A. cantonensis infection by an indirect enzyme-linked immunosorbent assay. The results showed that the Ac16 antibody had a 79.17% sensitivity to rAC16 and 83.33% to crude adult worm antigens (CA) (P > 0.05), while the specificity to rAC16 and to CA were 95.89% and 86.30% respectively (P < 0.05), thus implying that rAc16 may constitute a putative serodiagnostic antigen for Angiostrongyliasis cantonensis.

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Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Angiostrongylus cantonensis

Cited by 27 publications

References 4 publications

Corticosteroid Treatment of Eosinophilic Meningitis

Corticosteroid Treatment of Eosinophilic Meningitis

Detection of antibodies to Parastrongylus cantonensis in human sera by gelatin particle indirect agglutination test.

Cloning and characterization of a novel gene encoding 16 kDa protein (Ac16) from Angiostrongylus cantonensis

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