Enzyme-linked immunosorbent assay for human MRP-8/MRP-14 proteins and their quantitation in plasma of hematological patients

Ivanov, Gleb E.; Misuno, Natalia I.; Ivanov, Vadim E.

doi:10.1016/0165-2478(95)02472-7

Cited by 6 publications

(9 citation statements)

References 16 publications

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“…These facts support our hypothesis that the overexpression of MRP8/14 in human granulocytes in the inflammatory phase and its appearance in the serum is probably the result of inflammatory reactions associated with rejection in the transplanted tissues. Thus, the increase in CRP was not necessarily concomitant with that in MRP8/14 and may be attributable to bacterial infection in the recipient (18 ). Therefore, serum MRP8/14 seemed to be more sensitive for inflammatory responses associated with transplant rejection than was CRP.…”

Section: Discussionmentioning

confidence: 94%

“…To date, there have been few reports on the clinical importance of MRP8/14 as a marker of inflammatory reaction associated with transplant rejection in graft tissues, particularly in the acute phase (17,18 ). The present study was based on our hypothesis that MRP8/14 could serve as a useful marker for acute inflammation associated with rejection in the grafted tissues.…”

Section: Proteomics and Protein Markersmentioning

confidence: 99%

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New ELISA System for Myeloid-related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory Responses Associated with Transplant Rejection

et al. 2003

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Background: C-reactive protein (CRP), a useful marker for inflammatory diseases, is not always sensitive to inflammatory reaction in the liver or other tissues. The aim of this study was to develop a sensitive and specific method for detecting inflammatory responses associated with transplant rejection. Methods: We developed a new, highly sensitive ELISA system for the measurement of serum human myeloidrelated protein complex (MRP8/14), using monoclonal antibodies against MRP8/14, and applied it to specimens obtained from patients undergoing small intestine or liver transplantation. Results: This assay could detect MRP8/14 concentrations as low as 2 g/L. Within-run CVs were 3.7-6.1% and between-day CVs were 5.6 -8.7% for MRP8/14 concentrations of 117-3300 g/L. Mean recovery was 104% (range, 80 -128%). We observed a marked increase in serum MRP8/14 postoperatively in most recipients of transplants, followed by an increase in CRP 1-7 days after the increase in the complex. The increase in serum MRP8/14 occurred simultaneously with permeation of lymphocytes into the transplanted tissues as a result of rejection of the graft tissues. Conclusions: Accurate measurement of serum MRP8/14 provides a useful clinical diagnostic method tool for

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Section: Discussionmentioning

confidence: 94%

Section: Proteomics and Protein Markersmentioning

confidence: 99%

New ELISA System for Myeloid-related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory Responses Associated with Transplant Rejection

et al. 2003

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“…Using an ELISA developed with monoclonal antibodies to MRP8/14 [12], we determined the plasma levels of MRP8/14 in patients with active pulmonary TB and SR, and examined the relationship between these levels and the volume of lung tissue involved in the inflammatory process. In addition, we evaluated MRP8/14 release in Mycobacterium bovis bacillus Calmette‐Guérin (BCG)‐infected human monocytes and the direct effect of human MRP8/14 on Mycobacterium tuberculosis H37Rv growth in vitro.…”

Section: Introductionmentioning

confidence: 99%

Calprotectin (MRP8/14 protein complex) release during mycobacterial infection in vitro and in vivo

Pechkovsky

Zalutskaya

Ivanov³

et al. 2000

FEMS Immunology &Amp; Medical Microbiology

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The calprotectin (MRP8/14) protein complex belongs to the S100 family of Ca2+ binding proteins and is expressed during myelomonocytic differentiation. MRP8/14 plasma levels were determined by ELISA in 35 patients with active pulmonary tuberculosis (TB) showing mild (n = 12), moderate (n = 11) or severe (n = 12) disease, 13 patients with active pulmonary sarcoidosis (SR) and 21 healthy controls. TB patients had significantly increased plasma levels of MRP8/14 in comparison with SR and controls, which significantly depended on the volume of lung tissue involved in the inflammatory process. In TB patients, there was no correlation between plasma levels of MRP8/14 and total white blood cell (WBC) count, and blood polymorphonuclear neutrophil (PMN) count. In SR patients, MRP8/14 plasma levels were twofold higher in comparison with controls, but were lower compared with mild TB, and correlated with PMN and WBC counts. Human monocytes infected and cultured for 7 days with Mycobacterium bovis bacillus Calmette-Guérin showed fivefold higher MRP8/14 levels in supernatants compared with unstimulated or purified protein derivative-stimulated cells. Human MRP8/14 significantly increased Mycobacterium tuberculosis H37Rv growth in liquid medium in a dose- and time-dependent manner. These findings suggest that MRP8/14 plays an important role in the immunopathogenesis of tuberculosis.

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“…For example, S100A8/A9 plasma levels ranged from 1 to 15 µg/ml in leukemia patients [56]. In juvenile rheumatoid arthritis patients, S100A8 and S100A9 was estimated at significantly higher concentrations in synovial fluid (mean 42.8 µg/ml) compared with serum (2.1 µg/ml) [57].…”

Section: Cellular Functions Of S100a8/a9mentioning

confidence: 98%

Induction of Apoptotic Cell Death in Tumor Cells by S100A8/A9 Released from Inflammatory Cells Upon Cellular Activation

Ghavami¹

2005

CMCAIAA

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Polymorphonuclear neutrophils (PMNs) have received less attention than other leukocyte subsets as potential mediators of antitumor effects. However, there is a growing body of evidence that PMNs are potential mediators of antitumor effects and play an important role in the antitumor response. A protein complex S100A8/A9, formed by the two low molecular weight calcium-binding proteins S100A8 and S100A9, is released from activated PMNs and has apoptosis/cytotoxicity-inducing activity against tumor cell lines. In this review, we focus on the molecular mechanisms by which S100A8/A9 induces apoptosis. Recent studies point to a yet undefined mechanism, probably relaying on cell surface receptor(s), by which S100A8/A9 induces apoptosis in carcinoma cell lines, in addition to zinc exclusion from target cells. Although a number of putative receptors have been identified, the cell-membrane bindings site(s) involved in the apoptosis-inducing activity of S100A8/A9 remain unknown. The identification of the binding site(s) together with the elucidation of the underlying molecular mechanisms will give new insights in how the adaptive immune system is involved in cancer regression.

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Enzyme-linked immunosorbent assay for human MRP-8/MRP-14 proteins and their quantitation in plasma of hematological patients

Cited by 6 publications

References 16 publications

New ELISA System for Myeloid-related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory Responses Associated with Transplant Rejection

New ELISA System for Myeloid-related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory Responses Associated with Transplant Rejection

Calprotectin (MRP8/14 protein complex) release during mycobacterial infection in vitro and in vivo

Induction of Apoptotic Cell Death in Tumor Cells by S100A8/A9 Released from Inflammatory Cells Upon Cellular Activation

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