Enzyme-linked immunosorbent assays for measurement of reovirus immunoglobulin G, A, and M levels in serum

Abstract

Enzyme-linked immunosorbent assays were developed for the quantitation of anti-reovirus immunoglobulin G (IgG), IgA, and IgM in serum. The assays were specific, sensitive, and reproducible and measured antibodies to the reovirus group antigen. They should permit diagnosis of recent reovirus infection and longitudinal study of the development of reovirus group antibodies.

Prevalence of Reovirus‐Specific Antibodies in Young Children in Nashville, Tennessee

“…Other advantages are its low cost and use of small amounts of serum. Prior studies have shown ELISA to have limited serotype specificity with regard to reovirus: there is significant crossreactivity between all 3 reovirus serotypes [13,14]. This observation suggests that our assay was capable of detecting antibodies against reovirus serotypes other than type 3, although only antibodies directed against type 3 reovirus were specifically measured in the present study.…”