Enzyme-Linked Immunosorbent Spot (ELISpot) monitoring of cytokine-producing cells for the prediction of acute rejection in renal transplant patients

Mohammadi, Fatemeh; Solgi, Ghasem; Tajik, Maryam; Ahmadpoor, Pedram; Nikoeinejad, Hasan; Einollahi, Behzad; Nazari, Banafsheh; Lessan‐Pezeshki, Mahboob; Amirzargar, Aliakbar

doi:10.1684/ecn.2017.0397

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Interleukins (ILs) are a group of cytokines involved in the etiologies of many inflammation-related disorders, trigger inflammation-induced immune responses, such as pain, various infections and allergic reactions, and cell regeneration [1,2] . Meanwhile, an antibody directed against an IL-specific peptide shows anti-inflammatory functions [3] .…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, an antibody directed against an IL-specific peptide shows anti-inflammatory functions [3] . Of the IL family, IL-6 is a pro- and anti-inflammatory cytokine, responsible for the pathogenesis of many inflammation-related conditions, including infectious [1,4-6] and immunological disorders [2,7] . A recent review comprehensively described the profile of circulating IL-6 in relation to different cardiac operations [8] .…”

Section: Introductionmentioning

confidence: 99%

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Predictors of Normalization of Circulating Interleukin-6 after Cardiac Myxoma Resection

Yuan¹,

Lin²

2019

Braz J Cardiovasc Surg

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Objective To disclose the relationships between the anatomic features of cardiac myxomas and plasma interleukin (IL)-6 levels. Methods Twelve patients undergoing cardiac myxoma resection at The First Hospital of Putian, Teaching Hospital, Fujian Medical University were enrolled into this study. Pre- and postoperative IL-6 levels were determined by an enzyme-linked immunosorbent assay method, and correlations between cardiac myxoma dimension or volume and plasma IL-6 levels were analyzed. C-reactive protein (CRP) levels were also evaluated. Results IL-6 and CRP levels were significantly decreased one month after cardiac myxoma resection in comparison to preoperative values. IL-6 and CRP levels did not differ between patients with a cardiac myxoma of irregular appearance and those with a myxoma of regular gross appearance, or between patients with a pedicled or a sessile myxoma. Decrement of IL-6 of patients with irregular cardiac myxomas was much higher than that of patients with regular ones, while no intergroup difference was noted in decrement of CRP. A close direct correlation was noted between IL-6 levels and maximal dimension (length) or volume of cardiac myxomas, whereas CRP levels only correlated with maximal dimension of cardiac myxomas. Conclusion Anatomic features of cardiac myxomas (sessile, irregular appearance, maximal dimension, and volume) could be determinants of the patients' circulating IL-6 levels. IL-6 was likely to be a more sensitive biomarker than CRP in predicting the inflammatory status of patients with cardiac myxoma. Sessile and irregular cardiac myxomas might predict more severe inflammatory conditions for their more abundant endothelial cells and IL-6 overproduction.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Predictors of Normalization of Circulating Interleukin-6 after Cardiac Myxoma Resection

Yuan¹,

Lin²

2019

Braz J Cardiovasc Surg

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“…[10][11][12][13][14] AR has been associated with high numbers of donor-reactive IFN-γ and IL-21 memory T cells before transplantation or at 3-6 mo after transplantation. 8,9,[15][16][17][18] However, the dynamics of donorreactive cells during the process of AR remains unknown. By measuring donor-reactive cells at the time of biopsy sampling, we may be able to correlate these cell numbers in the peripheral blood to specific clinical events.…”

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confidence: 99%

Alloreactive T cells to Assess Acute Rejection Risk in Kidney Transplant Recipients

Rojas

Verhoeven

Kuiper

et al. 2023

Transplantation Direct

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Background. Memory T cells are important mediators of transplant rejection but are not routinely measured before or after kidney transplantation. The aims of this study were as follows: (1) validate whether pretransplant donor-reactive memory T cells are reliable predictors of acute rejection (AR) (2) determine whether donor-reactive memory T cells can distinguish AR from other causes of transplant dysfunction. Methods. Samples from 103 consecutive kidney transplant recipients (2018–2019) were obtained pretransplantation and at time of for-cause biopsy sampling within 6 mo of transplantation. The number of donor-reactive interferon gamma (IFN-γ) and interleukin (IL)-21-producing memory T cells was analyzed by enzyme-linked immunosorbent spot (ELISPOT) assay. Results. Of the 63 patients who underwent a biopsy, 25 had a biopsy-proven acute rejection (BPAR; 22 aTCMR and 3 aAMR), 19 had a presumed rejection, and 19 had no rejection. Receiver operating characteristic analysis showed that the pretransplant IFN-γ ELISPOT assay distinguished between patients who later developed BPAR and patients who remained rejection-free (area under the curve [AUC] 0.73; sensitivity 96% and specificity 41%). Both the IFN-γ and IL-21 assays were able to discriminate BPAR from other causes of transplant dysfunction (AUC 0.81; sensitivity 87% and specificity 76% and AUC 0.81; sensitivity 93% and specificity 68%, respectively). Conclusions. This study validates that a high number of donor-reactive memory T cells before transplantation is associated with the development of AR after transplantation. Furthermore, it demonstrates that the IFN-γ and IL-21 ELISPOT assays are able to discriminate between patients with AR and patients without AR at the time of biopsy sampling.

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“…INF-γ and IL-17 inhibit the generation and function of Treg cells, and thus have a detrimental effect on transplant immunity. 30 In addition, IL-10 was a well-known immunosuppressive cytokine that plays a key role in immune tolerance. 31 Consistent with these prior studies, we observed a reduction of INF-γ and IL-17 and increase of IL-10 levels in the DC-Dectin-1-RNAi group.…”

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confidence: 99%

Inhibition of Dectin-1 on Dendritic Cells Prevents Maturation and Prolongs Murine Islet Allograft Survival

Ren¹,

Li²,

Zhang³

et al. 2021

JIR

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Introduction: The ability of dendritic cells (DCs) to initiate an immune response or induce immune tolerance depends on their maturation status. Dendritic-cell-associated C-type lectin 1 (Dectin-1) plays a key role in the differentiation, activation, and maturation of DCs. Therefore, we hypothesized that inhibition of Dectin-1 could prevent DC maturation and induce immune tolerance of transplanted organs. Methods: DCs were transduced with a recombinant lentiviral vector to inhibit Dectin-1 and then were injected into a murine recipient before islet transplantation. C57BL/6 mice (H-2b) were treated with lentiviral vector-Dectin-1-RNAi-DC (DC-Dectin-1-RNAi group), lentiviral vector-GFP DCs (DC-GFP group), and PBS (control group). Pancreatic islet transplantation was performed and graft survival was recorded. The proportions of regulatory T cells, Th1 cells, and Th17 cells in the spleen and draining lymph nodes, and serum levels of interleukin (IL)-10, IL-17, and interferon (INF)-γ were measured. Results: The inhibition of Dectin-1 resulted in low expression of MHC-II and costimulatory molecules in DCs. Murine recipients treated with DC-Dectin-1-RNAi had longer islet allograft survival time, a reduction in the levels of Th1 and Th17 cells and secreted cytokines, and an increase of Treg cells. Conclusion: The inhibition of Dectin-1 by recombinant lentiviral vector Dectin-1-RNAi inhibits the maturation and activation of DCs, affects the differentiation of T cell subsets, and prolongs allograft survival.

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Enzyme-Linked Immunosorbent Spot (ELISpot) monitoring of cytokine-producing cells for the prediction of acute rejection in renal transplant patients

Cited by 6 publications

References 23 publications

Predictors of Normalization of Circulating Interleukin-6 after Cardiac Myxoma Resection

Predictors of Normalization of Circulating Interleukin-6 after Cardiac Myxoma Resection

Alloreactive T cells to Assess Acute Rejection Risk in Kidney Transplant Recipients

Inhibition of Dectin-1 on Dendritic Cells Prevents Maturation and Prolongs Murine Islet Allograft Survival

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