Enzyme-modified electrolyte-gated organic field-effect transistors

Buth, Felix; Donner, A.; Stutzmann, M.; Garrido, José Antonio

doi:10.1117/12.929850

Cited by 3 publications

(1 citation statement)

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“…Examples of receptor systems included in OFET sensor structures (Figure ) include DNA hybridization, selective detection of DNA via surface-bound peptide nucleic acid, lactate oxidase or diamine oxidase combined with horseradish peroxidase, enzyme-linked immunosorbent assay to create a preeclampsia prognostic, inclusion of a streptavidin protein capturing interlayer aiming for label-free electronic bioanalytical detection, ion-selective membranes on top of the semiconductor for the selective detection of alkali ions, addition of a monolayer of the synthetic cucurbit[6]uril onto the semiconductor surface for the detection of the neurotransmitter ACh down to 10 –12 M concentrations, and phenolic receptors to detect the nerve agent simulant dimethyl methylphosphonate . EGOFETs operated in the gating-through-water configuration including various anchored receptors along the organic–semiconductor surface facing the aqueous medium have been explored, such as to detect and monitor pH, DNA, penicillin, dopamine, and ions . Moreover, phospholipid layers have been utilized along the aqueous–semiconductor surface, , potentially enabling monitoring of reactions in transmembrane proteins.…”

Section: Organic Bioelectronics Applicationsmentioning

confidence: 99%

Organic Bioelectronics: Bridging the Signaling Gap between Biology and Technology

et al. 2016

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The electronics surrounding us in our daily lives rely almost exclusively on electrons as the dominant charge carrier. In stark contrast, biological systems rarely use electrons but rather use ions and molecules of varying size. Due to the unique combination of both electronic and ionic/molecular conductivity in conducting and semiconducting organic polymers and small molecules, these materials have emerged in recent decades as excellent tools for translating signals between these two realms and, therefore, providing a means to effectively interface biology with conventional electronics-thus, the field of organic bioelectronics. Today, organic bioelectronics defines a generic platform with unprecedented biological recording and regulation tools and is maturing toward applications ranging from life sciences to the clinic. In this Review, we introduce the field, from its early breakthroughs to its current results and future challenges.

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Section: Organic Bioelectronics Applicationsmentioning

confidence: 99%

Organic Bioelectronics: Bridging the Signaling Gap between Biology and Technology

et al. 2016

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Current‐Driven Organic Electrochemical Transistors for Monitoring Cell Layer Integrity with Enhanced Sensitivity

Lieberth

Romele

Torricelli

et al. 2021

Adv Healthcare Materials

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In this progress report an overview is given on the use of the organic electrochemical transistor (OECT) as a biosensor for impedance sensing of cell layers. The transient OECT current can be used to detect changes in the impedance of the cell layer, as shown by Jimison et al. To circumvent the application of a high gate bias and preventing electrolysis of the electrolyte, in case of small impedance variations, an alternative measuring technique based on an OECT in a current-driven configuration is developed. The ion-sensitivity is larger than 1200 mV V -1 dec -1 at low operating voltage. It can be even further enhanced using an OECT based complementary amplifier, which consists of a p-type and an n-type OECT connected in series, as known from digital electronics. The monitoring of cell layer integrity and irreversible disruption of barrier function with the current-driven OECT is demonstrated for an epithelial Caco-2 cell layer, showing the enhanced ion-sensitivity as compared to the standard OECT configuration. As a state-of-the-art application of the current-driven OECT, the in situ monitoring of reversible tight junction modulation under the effect of drug additives, like poly-l-lysine, is discussed. This shows its potential for in vitro and even in vivo toxicological and drug delivery studies.

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