Enzyme production by the mixed fungal culture with nano‐shear pretreated biomass and lignocellulose hydrolysis

Abstract

Cellulase, xylanase, and β-glucosidase production was studied on novel nano-shear pretreated corn stover by the mixed fungi culture. The high shear force from a modified Tayor-Couette nano-shear mixing reactor efficiently disintegrated corn stover, resulting in a homogeneous watery mash with particles in much reduced size. Scanning electron microscope study showed visible mini-pores on the fiber cell wall surface, which could improve the accessibility of the pretreated corn stover to microorganisms. Mixed fungal culture of Trichoderma reesei RUT-C30 and Aspergillus niger produced enzymes with higher cellulolytic and xylanolytic activities on corn stover pretreated with nano-shear mixing reactor, in comparison with other pretreatment methods, including acid and ammonia fiber explosion (AFEX) pretreatment. The hydrolytic potential of the whole fermentation broth from the mixed fungi was studied, and the possibility of applying the whole cell saccharification concept was also investigated to further reduce the cost of lignocellulose hydrolysis.

Activating Intrinsic Carbohydrate-Active Enzymes of the Smut Fungus Ustilago maydis for the Degradation of Plant Cell Wall Components

“…The potential of mixed cultures in biomass degradation has been described for diverse cases and mirrors nature where microbial communities cooperate in degrading plant matter (76,77).…”

Innovative Consortia of Micro and Macro Fungal Systems: Cellulolytic Enzyme Production from Groundnut Shell Biomass and Supportive Structural Analysis

“…The strains of Aspergillus are known to have high β-glucosidase activity release [19]. Mixed cultures are known to produce enzyme cocktails which are rich in β-glucosidase; thus diminishing the accumulated cellobiose concentration as a result of formation of glucose [20].…”

Direct Succinic Acid Production from Minimally Pretreated Biomass Using Sequential Solid-State and Slurry Fermentation with Mixed Fungal Cultures

“…Prior studies have shown that Trichoderma reesei and Aspergillus spp. co-cultures are more effective for maximizing cellulolytic enzyme activities than single cultures by establishing synergistic levels of exocellulases (produced primarily by T. reesei) and endocellulases and β-glucosidases (produced mainly by A. niger) for sustained enzymatic hydrolysis and minimization of product inhibition effects [6][7][8][9]. On the other hand, the C-rich substrate was pre-fermented using Phanerochaete chrysosporium, which has been shown to be effective in delignifying agricultural biomass residues [10,11].…”