Enzyme Production from Food Wastes Using a Biorefinery Concept

Kıran, Esra Uçkun; Trzcinski, Antoine P.; Ng, Wun Jern; Liu, Yu

doi:10.1007/s12649-014-9311-x

Cited by 87 publications

(27 citation statements)

References 105 publications

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“…The higher activity of the liquid biopreparation is an advantage; however, the biopreparation has a shorter expiration date, which indicates the maximum maintenance of its catalytic activity (WesolowskaTrojanowska and Targonski 2014). Nonetheless, motivations for using onsite-produced enzymes in biorefineries are to avoid expenditures and the loss of its activity during lyophilization (Kiran et al 2014).…”

Section: The Characteristics Of the Biopreparationmentioning

confidence: 99%

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Oszust¹,

Pawlik²,

Janusz³

et al. 2017

BioResources

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A multi-enzymatic biopreparation of Trichoderma atroviride G79/11 origin was characterized. The fungus showed relatively high cellulase production in a soybean flour-cellulose-lactose medium. Subsequently, based on its post-culture liquid, the biopreparation of the enzyme mixture was developed and characterized. The liquid form of the enzyme mixture reached 22 U cm -3 of cellulolytic activity and its lyophilisate exhibited 1.09 U cm -3 at pH 5.1 and 50 °C. The enzyme mixture was characterized by the following activities: xylanase, β-glucosidase, carboxymethyl cellulase, polygalactouronase, pectinesterase, amylase, lactase, and protease. A method for an efficient conditioning process of organic waste (fruit processing waste, dairy sewage sludge, corn silage, and grain broth) for biogas yield enhancement using the enzyme mixture was proposed. The enzyme mixture increased the efficacy of biogas production by 30% when the lyophilizate (0.5 mg g -1 d.m.) was applied prior to fermentation. A method for conducting the enzymatic conditioning process of organic waste using the enzyme mixture as a pretreatment was proposed. This was part of the optimization of the methane fermentation process to increase the biogas yield. Consequently, after application of the biopreparation, the efficiency of anaerobic digestion of organic waste was improved.

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Section: The Characteristics Of the Biopreparationmentioning

confidence: 99%

Characterization and Influence of a Multi-enzymatic Biopreparation for Biogas Yield Enhancement

Oszust¹,

Pawlik²,

Janusz³

et al. 2017

BioResources

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“…There are four groups of enzymes identified to be relevant to biorefineries: amylases, cellulases, xylanases and lignin‐peroxidases (Table ) . These have applications in industries such as textile, food, pulp and paper, biofuel, inter alia.…”

Section: Enzymes For Biomass Conversionmentioning

confidence: 99%

“…There are indeed many exhaustive reviews that have successfully and thoroughly covered each type of enzyme, kinetics, mechanism of action, substrates, products and their producing microorganisms, therefore, the following subsections will only cover an overview of the most important facts concerning these enzymes.…”

Section: Enzymes For Biomass Conversionmentioning

confidence: 99%

“…These enzymes act synergistically to effectively depolymerize cellulose . Whereas endoglucanases attack β‐1,4 linkages in the amorphous regions inside the glucan chain, cellobiohydrolases detach cellobiose from reducing and non‐reducing ends, and β‐glucosidases convert the latter to glucose monomers …”

Section: Enzymes For Biomass Conversionmentioning

confidence: 99%

“…The α‐amylases cleave the 1,4‐α‐D‐glucosidic linkages between adjacent glucose units in the linear amylose chain of starch, yielding maltose, glucose and maltotriose (Table ). Glucoamylase hydrolyses the non‐reducing ends of amylose and amylopectin to glucose . Amylases are used in the food, fermentation, textile and paper industries, as well as for the pretreatment of agroindustrial and organic by‐products to increase bioproduct yield in downstream processes.…”

Section: Enzymes For Biomass Conversionmentioning

confidence: 99%

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An overview of the enzyme potential in bioenergy-producing biorefineries

Escamilla‐Alvarado

Perez-Pimienta

Ponce-Noyola

et al. 2016

J. Chem. Technol. Biotechnol.

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Biorefineries are considered as an integrative thinking that focuses on the possibility of obtaining as many added-value products as technically and economically feasible. However, in practice most biorefineries comprise only enzymatic or chemical pretreatment followed by biofuel generation. The drop in oil prices may menace the development of this young industry, as has happened before in history. This has become a fundamental reason for which the biofuel industry should not consider only biofuels production, but enzyme and non-fuel based chemicals as well. Hence, this work aims at overviewing the most important enzymes involved in biotechnological processes and to describe their role in biorefineries. Bioethanol, biogas and biodiesel biorefineries are overviewed, along with the integrated and industrial types. Finally separation and purification processes in biorefineries are discussed.

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