Enzyme Profiles of &amp;lt;i&amp;gt;Pleurotus&amp;lt;/i&amp;gt; HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure

Raymond, Prosper

doi:10.11648/j.ab.20150305.12

Cited by 5 publications

(1 citation statement)

References 27 publications

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“…These results are reliable with those of [1], as it used commercial agar on PDA media for culture of Pleurotus HK-37. Raymond et al [26] also described the culture of Pleurotus HK-37 using malt extract agar (MEA) media; the results for growth rate of Pleurotus HK-37 were the same as those of this study. Moreover, this study shows that mycelia growth rate of Pleurotus HK-37 in PDA culture media is affected not only by substrates and nutrients which exist in media as reported by Mshandete [1] but also by the gel strength of agar.…”

Section: Discussionsupporting

confidence: 70%

Performance of Low-Cost Agar fromGracilaria salicorniaon Tissue Culture ofPleurotusHK-37

Mpatani

Vuai

2019

The Scientific World Journal

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Currently, the demand of Pleurotus HK-37 (oyster mushroom) in Tanzania is growing rapidly due to the increasing of awareness on its nutrition, health, and economic benefits. Despite the increasing demand, the availability of strains of Pleurotus HK-37 species is still a challenge due to high cost of tissue culture technology. The high cost of importing agar seems to be among the factors for this failure. This study aimed at investigating the performance of low-cost agar from local Gracilaria salicornia on tissue culture of Pleurotus HK-37. Local extracted agars with different gel strengths ranging between 100, 200, 300, 400, and 500 g/cm2 were used to make PDA media. The average mycelia growth rate (mm/day) ranged between 9.87 ± 1.44 and 14.9 ± 0.85 mm/day. Low-cost agar shows quite similar performance as that of standard agar on active growth of Pleurotus HK-37 mycelia. All PDA plates appeared white and feathery and showed to grow in a circular mode (radial extension). Mycelia growth on standard agar PDA took 5 days while on extracted local agar PDA took 5 to 7 days to fully colonize the plate at 27 ± 2°C. The present study shows that the production cost can be reduced by ∼35–78% by using local agar.

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Section: Discussionsupporting

confidence: 70%