Enzyme reaction-guided identification of active components from the flowers of <i>Sophora japonica</i> var. violacea

Zhang, Yunci; Gu, Dongyu; He, Shuai; Meng, Jing; Wang, Jifeng; Wang, Yunxiao; Wang, Yi; Tian, Jing; Yang, Yi

doi:10.1039/d0fo00625d

Cited by 13 publications

(17 citation statements)

References 41 publications

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“…Oxysophoridine 242 exhibited a dose-dependent inhibition of 2019-CoV replication in infected cells similar to chloroquine, the reported reference drug (Zhang et al 2020a , b , c ). The compound 242 may have a broad-spectrum antiviral activity against RNA viruses.…”

Section: Biological Effectsmentioning

confidence: 61%

“…Coronavirus disease 2019 (COVID-19) is an illness caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (Zhang et al 2020a , b , c ; Majnooni et al 2021 ). The ongoing COVID-19 pandemic has created an alarming situation due to its high morbidity and mortality rates, economic losses, and the threat to global health security.…”

Section: Biological Effectsmentioning

confidence: 99%

“…In the last decades, the applications of gemcitabine as a potent antiviral and chemotherapy agent attracted growing attention. Gemcitabine exhibited in vitro activity against SARS-CoV-2, with EC 50 = values of 1.24 µM (Zhang et al 2020a , b , c ; Batalha et al 2021 ). A combination of gemcitabine with 242 had an antiviral effect against SARS-CoV-2 (Zhang et al 2020a , b , c ).…”

Section: Biological Effectsmentioning

confidence: 99%

“…Gemcitabine exhibited in vitro activity against SARS-CoV-2, with EC 50 = values of 1.24 µM (Zhang et al 2020a , b , c ; Batalha et al 2021 ). A combination of gemcitabine with 242 had an antiviral effect against SARS-CoV-2 (Zhang et al 2020a , b , c ).…”

Section: Biological Effectsmentioning

confidence: 99%

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Genus Sophora: a comprehensive review on secondary chemical metabolites and their biological aspects from past achievements to future perspectives

2021

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Sophora is deemed as one of the most remarkable genera of Fabaceae, and the third largest family of flowering plants. The genus Sophora comprises approximately 52 species, 19 varieties, and 7 forms that are widely distributed in Asia and mildly in Africa. Sophora species are recognized to be substantial sources of broad spectrum biopertinent secondary metabolites namely flavonoids, isoflavonoids, chalcones, chromones, pterocarpans, coumarins, benzofuran derivatives, sterols, saponins (mainly triterpene glycosides), oligostilbenes, and mainly alkaloids. Meanwhile, extracts and isolated compounds from Sophora have been identified to possess several health-promising effects including anti-inflammatory, anti-arthritic, antiplatelets, antipyretic, anticancer, antiviral, antimicrobial, antioxidant, anti-osteoporosis, anti-ulcerative colitis, antidiabetic, anti-obesity, antidiarrheal, and insecticidal activities. Herein, the present review aims to provide comprehensive details about the phytochemicals and biological effects of Sophora species. The review spotlighted on the promising phytonutrients extracted from Sophora and their plethora of bioactivities. The review also clarifies the remaining gaps and thus qualifies and supplies a platform for further investigations of these compounds.

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Section: Biological Effectsmentioning

confidence: 61%

Section: Biological Effectsmentioning

confidence: 99%

Section: Biological Effectsmentioning

confidence: 99%

Section: Biological Effectsmentioning

confidence: 99%

See 2 more Smart Citations

Genus Sophora: a comprehensive review on secondary chemical metabolites and their biological aspects from past achievements to future perspectives

2021

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“…And two biocompounds (defined as F2 and J2) exhibited excellent antibacterial activity. In order to investigate the chemical structure of two biocompounds, they were identified by LC-MS [23], 1H-NMR, and 13C-NMR [24]. Moreover, to further confirm the antibacterial activity of F2, more antibacterial experiments were carried out.…”

Section: Introductionmentioning

confidence: 99%

Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application

Qiu

Yang

et al. 2020

Eur Food Res Technol

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Natural biologically active substances have received continuous attention for the potentially beneficial health properties against chronic diseases. In this study, bacteriostatic active substance from Camellia oleifera meal, which is a major byproduct of the Camellia oil processing industry, were extracted with continuous phase change extraction (CPCE) method and separated by HSCCC. Compared with traditional extraction methods, CPCE possessed higher extraction efficiency. Two main substances were separated and purified (above 90.0%). The structure of them were further identified by UV, LC-ESI-MS-MS, 1H-NMR, and 13C-NMR as flavonoids F2 kaempferol 3-O-[β-d-glucopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-dglucopyranoside and J2 kaempferol 3-O-[β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl-(1 → 6)]-β-d-glucopyranoside for the first time in C. Oleifera meal. The results of antibacterial activity measurement showed that both compounds have excellent antibacterial activity. And the antibacterial stability of F2 were finally confirmed: F2 showed broad spectrum antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella enteriditis, Bacillus thuringiensis, Aspergillus niger and Rhizopus nigricans. Besides, F2 exhibited relatively high stable property even at high temperature, acid and metal ion solutions. The findings of this work suggest the possibility of employing C. oleifera meal as an attractive source of health-promoting compounds, and at the same time facilitate its high-value reuse and reduction of environmental burden.

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Dual‐target affinity analysis and separation of α‐amylase and α‐glucosidase inhibitors from Morus alba leaves using a magnetic bifunctional immobilized enzyme system

Guo

Liu

Meng

et al. 2022

Biomedical Chromatography

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Morus alba leaves are a natural product with great antidiabetic potential. However, the therapeutic efficacy of natural products is usually achieved through the interaction of active compounds with specific targets. Among them, active compounds with multi‐target therapeutic functions are more effective than single‐target enzymes. In this study, a bienzyme system was constructed by co‐immobilizing α‐amylase and α‐glucosidase onto Fe3O4 for affinity screening of dual‐target active components in the complex extract from M. alba leaves. As a result, a potential active compound was selectively screened by ligand fishing, separated by high‐speed countercurrent chromatography using a solvent system of ethyl acetate–n‐butanol–water (3:2:5, v/v), and identified as rutin. In addition, the result of molecular docking showed that rutin could interact with the active center of α‐amylase and α‐glucosidase through multiple hydrogen bonds, van der Waals forces, etc. to play an inhibitory role. These results demonstrate the effectiveness of the polydopamine magnetically immobilized bienzyme system for dual‐target affinity screening of active substances. This study not only reveals the chemical basis of the antidiabetic activity of M. alba leaves from a dual‐target perspective, but also promotes the progress of multitarget affinity screening.

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Enzyme reaction-guided identification of active components from the flowers of Sophora japonica var. violacea

Abstract: The flower of S. japonica is a favorite food and used as traditional medicine.

Cited by 13 publications

References 41 publications

Genus Sophora: a comprehensive review on secondary chemical metabolites and their biological aspects from past achievements to future perspectives

Genus Sophora: a comprehensive review on secondary chemical metabolites and their biological aspects from past achievements to future perspectives

Kaempferol separated from Camellia oleifera meal by high-speed countercurrent chromatography for antibacterial application

Dual‐target affinity analysis and separation of α‐amylase and α‐glucosidase inhibitors from Morus alba leaves using a magnetic bifunctional immobilized enzyme system

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