Enzyme Reaction in the Pores of CaCO₃ Particles upon Ultrasound Disruption of Attached Substrate‐Filled Liposomes

Abstract

Breaking out: Liposomes that are adsorbed onto porous CaCO3 particles and that contain the peroxidase substrate Amplex Red (AR) are disrupted by ultrasonic treatment. The substrate is released and diffuses into the inner part of the multicompartment containers where the peroxidase enzyme is found, and the enzymatic reaction is triggered. This approach may be useful for the simultaneous delivery of multiple molecules into cells.

Raman imaging and photodegradation study of phthalocyanine containing microcapsules and coated particles

“…[30] It can be noted also that current studies are useful to further extend the application of a variety of PDT [48] agents, to investigate the stability of molecules, and can be also used for practical applications, particularly those related to controlled permeability changes. [49] Furthermore, the studied phenomena are of use for recent developments in the area of bio-inspired colloidal systems, [44] multicompartment [50,51] and anisotropic constructs, [52,53] and planar polymeric films. [54 -58] The organic molecules reported here can be used for reducing the threshold of activation of microcapsules, [59] although metal nanoparticles [60] as well as carbon nanotubes [61] still remain attractive alternatives because they are used not only for remote release but also for improving mechanical properties of microcapsules.…”

Pharmacological aspects of release from microcapsules — from polymeric multilayers to lipid membranes

“…Microcapsules containing enzymes have attracted particular attention [101]. Microcapsules possessing both a substrate and a corresponding enzyme in the same structure are yet another important step toward designing advanced drug delivery carriers [102]. Microcapsule fusion may first of all be of interest for studying the state of molecules inside polyelectrolyte multilayers (e.g.…”

Colloidal micro- and nano-particles as templates for polyelectrolyte multilayer capsules

“…However, the particles on which capsules are templated can also be used as carriers of enzymes. The polyelectrolyte multilayer shell can be constructed over such particles loaded with enzymes, providing an important functionality -protection of enzymes, just as in the case of capsules [75].…”