Pharmacological aspects of release from microcapsules — from polymeric multilayers to lipid membranes

Wuytens, Pieter; Parakhonskiy, Bogdan; Yashchenok, Alexey M.; Winterhalter, Mathias; Skirtach, André G.

doi:10.1016/j.coph.2014.09.016

Cited by 22 publications

(18 citation statements)

References 138 publications

(140 reference statements)

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“…nano-and microparticles, liposomes and red blood cells, represent an important class of vehicles which are often designed for mimicking naturally occurring delivery systems. Polymeric capsules engineered through the highly 4 versatile Layer-by-Layer (LbL) technique have attracted particular attention in the last decades, because they offer flexibility of design and multifunctionality, and they can be used in a broad range of drug delivery applications [30][31][32] . The LbL technique is based on the consecutive adsorption of oppositely charged polyelectrolytes on a preformed charged template core which is chemically dissolved after the coating process.…”

Section: Introductionmentioning

confidence: 99%

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles

Donatan

Yashchenok

Khan

et al. 2016

ACS Appl. Mater. Interfaces

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A new method of fabrication of calcium carbonate microparticles of ellipsoidal, rhomboidal and spherical geometries is reported by adjusting the relative concentration ratios of the initial salt solutions and/or the ethylene glycol content in the reaction medium. Morphology, porosity, crystallinity and loading capacity of synthesized CaCO3 templates were characterized in detail. Particles harbouring dextran or the enzyme guanylate kinase were obtained through encapsulation of these macromolecules using the layer-by-layer assembly technique to deposit positively and negatively charged polymers on these differently shaped CaCO3 templates and were characterized by confocal laser scanning fluorescence microscopy, fluorometric techniques, and enzyme activity measurements. The enzymatic activity – an important application of such porous particles and containers – has been analyzed in comparison to the loading capacity and geometry. Our results reveal that the particles' shape influenced on morphology of particles and as result affects the activity of the encapsulated enzyme, in addition to the earlier reported influence on cellular uptake. These particles are promising candidates for efficient drug delivery due to their relatively high loading capacity, biocompatibility, and easy fabrication and handling

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Section: Introductionmentioning

confidence: 99%

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles

Donatan

Yashchenok

Khan

et al. 2016

ACS Appl. Mater. Interfaces

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“…74,75 A number of various proteins such as aprotinin and insulin have been successfully loaded into crystals by ADS and COS. 74,75 The morphology of the crystals may affect the protein loading, 76 which is of high importance for pharmacological applications. 77 Although the potential for the utilization of crystals as carriers for protein loading is obvious, the loading mechanism through co-synthesis is poorly understood. Our recent works demonstrated some new findings on the interaction between protein molecules and crystals; however, the loading mechanism is still unknown.…”

Section: -33mentioning

confidence: 99%

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis

Vikulina

Feoktistova

Балабушевич

et al. 2018

Phys. Chem. Chem. Phys.

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Porous vaterite CaCO 3 crystals are nowadays extensively used as high-capacity bio-friendly sacrificial templates for the fabrication of such protein-containing nano-and micro-particles as capsules and beads. The first step in the protein encapsulation is performed through loading of the protein molecules into the crystals. Co-synthesis is one of the most useful and simple methods proven to effectively load crystals with proteins; however, the loading mechanism is still unknown. To understand the mechanism, in this study, we focus on the loading of a model protein catalase into the crystals by means of adsorption into pre-formed crystals (ADS) and co-synthesis (COS). Analysis of the physico-chemical characteristics of the protein in solution and during the loading and simulation of the protein packing into the crystals are performed. COS provides more effective loading than ADS giving protein contents in the crystals of 20.3 and 3.5 w/w%, respectively. Extremely high loading for COS providing a local protein concentration of about 550 mg mL À1 is explained by intermolecular protein interactions, i.e. by adsorption of the aggregates into the crystals. We believe that this study will be useful for protein encapsulation through CaCO 3 crystals using the COS method.

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“…LbL assembled polymer microcapsules are proven as a perspective for various applications due to versatility concerning the payload, targeted delivery, and controlled release options . Polyelectrolyte microcapsules are able to retain a variety of payloads with different water solubilities and perform their release by diffusion or burst release forced by remote activation with light, magnetic field, ultrasound, temperature, pH, salinity, redox potential, and chemicals . Hereby, such type of carriers holds out the prospect of their application for lipase encapsulation.…”

Section: Methodsmentioning

confidence: 99%

Optical monitoring of adipose tissue destruction under encapsulated lipase action

Yanina

Svenskaya

Prikhozhdenko

et al. 2018

Journal of Biophotonics

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Enzymatic destruction of adipose tissue has been achieved by encapsulation of lipase into the polymeric microcapsules. Adipose tissue destruction was delayed while lipase is encapsulated comparing with the direct lipase action as demonstrated by optical microscopy and optical coherence tomography in in vitro studies. Raman spectroscopy confirms that triglycerides in fat tissue were cleaved into free fatty acids, glycerol, and possible di- and monoglyceride residues. The results underpin the concept of local and controlled treatment of tissues via encapsulation. Effect of lipase encapsulation into the polymeric microcapsules on adipose tissue destruction compared to free lipase application.

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Pharmacological aspects of release from microcapsules — from polymeric multilayers to lipid membranes

Cited by 22 publications

References 138 publications

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis

Optical monitoring of adipose tissue destruction under encapsulated lipase action

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Pharmacological aspects of release from microcapsules — from polymeric multilayers to lipid membranes

Cited by 22 publications

References 138 publications

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles

The mechanism of catalase loading into porous vaterite CaCO3 crystals by co-synthesis

Optical monitoring of adipose tissue destruction under encapsulated lipase action

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Product

Resources

About

The mechanism of catalase loading into porous vaterite CaCO₃ crystals by co-synthesis