Optical monitoring of adipose tissue destruction under encapsulated lipase action

Abstract

Enzymatic destruction of adipose tissue has been achieved by encapsulation of lipase into the polymeric microcapsules. Adipose tissue destruction was delayed while lipase is encapsulated comparing with the direct lipase action as demonstrated by optical microscopy and optical coherence tomography in in vitro studies. Raman spectroscopy confirms that triglycerides in fat tissue were cleaved into free fatty acids, glycerol, and possible di- and monoglyceride residues. The results underpin the concept of local and controlled treatment of tissues via encapsulation. Effect of lipase encapsulation into the polymeric microcapsules on adipose tissue destruction compared to free lipase application.

Optimal hyperosmotic agents for tissue immersion optical clearing in terahertz biophotonics

“…Third, 100% and solute glycerol possesses the largest D and characterized by low-to-moderate α, which makes this agent optimal, when the fastest IOC of tissues with Moreover, biological tissues have rather high affinity for glycerol, which is an important structural element of a number of lipids and is a metabolic product of fat cell lipolysis. [43] Thus, in terms of toxicity, [44][45][46] it makes glycerol more preferable among the considered shortened list of agents for the IOC in vivo applications. The proposed nomogram-based approach seems to be quite convenient for an objective comparison of agent efficiency.…”

“…Moreover, biological tissues have rather high affinity for glycerol, which is an important structural element of a number of lipids and is a metabolic product of fat cell lipolysis. [ 43 ] Thus, in terms of toxicity, [ 44–46 ] it makes glycerol more preferable among the considered shortened list of agents for the IOC in vivo applications.…”

Immersion optical clearing of adipose tissue in rats: ex vivo and in vivo studies

“…The present study refers to physiological conditions, so the data obtained can be used for in vivo clinical procedures. For example, the proposed OC approach can serve to monitor the destruction of adipose tissue under the action of an administered encapsulated lipase [10]. The demonstrated OC of VAT as a result of the action of OCAs and enhanced upon its physiological heating can be used for optical control of the delivery of encapsulated drugs [77].…”

“…OC of adipose tissue is insufficiently studied although this technology can be prospective for medical applications [9,10].…”

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

“…In addition to ICG, other resonance absorbers, such as plasmonic gold nanoparticles and their conjugates with ICG, are promising . Currently, we are conducting studies to increase the selectivity of photosensitizers and enzyme lipase by their microencapsulation for prolonged release in adipose tissues .…”