Enzyme SU-8 microreactors: simple tools for cell-culture monitoring

Talaei, Sara; Wal, P. D. van der; Ahmed, S.I.-U.; Liley, Martha; Rooij, N. F. de

doi:10.1007/s10404-015-1562-8

Cited by 14 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Miniaturized versions have been used as scanning probes to monitor the glucose and lactate metabolism of single cells 42,43 . Flow-through-type sensor systems have been devised to analyze cell culture conditions and metabolites in liquid samples downstream of cell cultures [44][45][46][47][48][49][50][51] . Silicon-based sensor chips for multi-parameter online monitoring have been mounted in a perfused cell culture unit 52 , and multiple sensing electrodes have been directly incorporated into transparent microfluidic systems to measure the glucose consumption of single cardiac cells 53 or the lactate production of cells of various types 54 and, combined with optical read-out, for monitoring cancer cell metabolism 55 .…”

Section: Introductionmentioning

confidence: 99%

Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks

et al. 2016

View full text Add to dashboard Cite

Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out functionalities. Here we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for 'body-on-a-chip' applications. The microfluidic platform is based on the technology of hanging-drop networks, which is designed for the formation, cultivation, and analysis of fluidically interconnected organotypic spherical three-dimensional (3D) microtissues of multiple cell types. The sensor modules were designed as small glass plug-ins featuring four platinum working electrodes, a platinum counter electrode, and an Ag/AgCl reference electrode. They were placed directly into the ceiling substrate from which the hanging drops that host the spheroid cultures are suspended. The electrodes were functionalized with oxidase enzymes to enable continuous monitoring of lactate and glucose through amperometry. The biosensors featured high sensitivities of 322 ± 41 nA mM − 1 mm − 2 for glucose and 443 ± 37 nA mM − 1 mm − 2 for lactate; the corresponding limits of detection were below 10 μM. The proposed technology enabled tissue-size-dependent, real-time detection of lactate secretion from single human colon cancer microtissues cultured in the hanging drops. Furthermore, glucose consumption and lactate secretion were monitored in parallel, and the impact of different culture conditions on the metabolism of cancer microtissues was recorded in real-time.

show abstract

Section: Introductionmentioning

confidence: 99%

Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks

et al. 2016

View full text Add to dashboard Cite

show abstract

“…These LOC devices are good examples of how changes at single cell level can be monitored upon different stimuli using electrochemical sensing. Several of other electrochemical LOC devices implemented for the detection of cell-secreted lactate were also reported [96,98,99,112,113]. Many of these electrochemical lactate biosensors are amperometric and operate at high applied potentials that vary between 0.45 V to 0.70 V and used to record the current changes caused by the electrochemical oxidation of H 2 O 2 and thus these biosensors suffer from low selectivity.…”

Section: Electrochemical Methods For Extracellular Metabolitesmentioning

confidence: 99%

Recent Progress in Lab-On-a-Chip Systems for the Monitoring of Metabolites for Mammalian and Microbial Cell Research

Dervisevic

Tuck

Voelcker

et al. 2019

Sensors

View full text Add to dashboard Cite

Lab-on-a-chip sensing technologies have changed how cell biology research is conducted. This review summarises the progress in the lab-on-a-chip devices implemented for the detection of cellular metabolites. The review is divided into two subsections according to the methods used for the metabolite detection. Each section includes a table which summarises the relevant literature and also elaborates the advantages of, and the challenges faced with that particular method. The review continues with a section discussing the achievements attained due to using lab-on-a-chip devices within the specific context. Finally, a concluding section summarises what is to be resolved and discusses the future perspectives.

show abstract

“…64,71–74 Frey et al fabricated a disposable PDMS cartridge for the measurement of glucose and lactate in solution featuring on-line adjustment of the linear response range and sensitivity of the biosensor 68. The same group later developed a novel cartridge sensor for cell-culture applications featuring a limit of detection of less than 5 nM 69…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…The flexibility provided by the enzyme functionalization has led to the realization of a large variety of biosensors for the analysis of cell cultures and metabolites in liquid samples . Typical examples for cell culture applications include glucose and lactate electrochemical biosensors, the sensing electrodes of which are functionalized with glucose oxidase or lactate oxidase. − Upon interaction of the oxidase enzyme with the target substrate, hydrogen peroxide is produced, which can then be detected using an amperometric detection scheme. Using an SECM featuring glucose or lactate enzymatic biosensors at the tip, Ciobanu et al were able to measure the glucose consumption of individual fibroblasts and the lactate secretion of single cancer cells cultured in standard Petri dishes .…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…Furthermore, several sensor types and units have been developed for the downstream analysis of cell culture medium. ,− Frey et al fabricated a disposable PDMS cartridge for the measurement of glucose and lactate in solution featuring online adjustment of the linear response range and sensitivity of the biosensor . The same group later developed a novel cartridge sensor for cell culture applications featuring a limit of detection of less than 5 nM . The increased sensitivity was obtained by maximizing the interaction between the solution and the oxidase enzymes by using a microfabricated pillar structure for increasing the functionalized surface area.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

See 1 more Smart Citation

Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems

et al. 2018

View full text Add to dashboard Cite

Technological advances in microfabrication techniques in combination with organotypic cell and tissue models have enabled the realization of microphysiological systems capable of recapitulating aspects of human physiology in vitro with great fidelity. Concurrently, a number of analysis techniques has been developed to probe and characterize these model systems. However, many assays are still performed off-line, which severely compromises the possibility of obtaining real-time information from the samples under examination, and which also limits the use of these platforms in high-throughput analysis. In this review, we focus on sensing and actuation schemes that have already been established or offer great potential to provide in situ detection or manipulation of relevant cell or tissue samples in microphysiological platforms. We will first describe methods that can be integrated in a straightforward way and that offer potential multiplexing and/or parallelization of sensing and actuation functions. These methods include electrical impedance spectroscopy, electrochemical biosensors, and the use of surface acoustic waves for manipulation and analysis of cells, tissue, and multicellular organisms. In the second part, we will describe two sensor approaches based on surface-plasmon resonance and mechanical resonators that have recently provided new characterization features for biological samples, although technological limitations for use in high-throughput applications still exist.

show abstract

Enzyme SU-8 microreactors: simple tools for cell-culture monitoring

Abstract: present inside the medium, a protocol for a toxicant-free sampling is investigated.

Cited by 14 publications

References 39 publications

Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks

Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks

Recent Progress in Lab-On-a-Chip Systems for the Monitoring of Metabolites for Mammalian and Microbial Cell Research

Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems

Contact Info

Product

Resources

About