Enzyme-treated <i>Asparagus officinalis</i> Extract Shows Neuroprotective Effects and Attenuates Cognitive Impairment in Senescence-accelerated Mice

Sakurai, Takeshi; Ito, Tomohiro; Wakame, Koji; Kitadate, Kentaro; Arai, Takashi; Ogasawara, Jun; Kizaki, Takako; Sato, Shogo; Ishibashi, Yoshinaga; Fujiwara, Tomonori; Akagawa, Kimio; Ishida, Hideyuki; Ohno, Hideki

doi:10.1177/1934578x1400900130

Cited by 20 publications

(24 citation statements)

References 43 publications

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“…We have recently developed a new functional material, enzyme‐treated asparagus extract (ETAS), from unused parts of asparagus ( Asparagus officinalis L.) grown in Hokkaido, Japan. Our current study revealed that ETAS shows neuroprotective effects and attenuates cognitive impairment in senescence‐accelerated mice (Sakurai and others ). Other study reported that ETAS contains 5‐hydroxymethyl‐2‐furfural (HMF) and its derivative, which is a novel compound and named asfural, as shown in Figure (Ito and others ).…”

Section: Introductionsupporting

confidence: 62%

Enzyme‐Treated Asparagus Extract Promotes Expression of Heat Shock Protein and Exerts Antistress Effects

Ito

Maeda

Goto

et al. 2014

Journal of Food Science

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A novel enzyme-treated asparagus extract (ETAS) has been developed as a functional material produced from asparagus stem. Studies were conducted to determine the effect of ETAS on heat shock protein 70 (HSP70) expression and alleviation of stress. HeLa cells were treated with ETAS, and HSP70 mRNA and protein levels were measured using a reverse transcription-polymerase chain reaction (RT-PCR) assay and an enzyme-linked immunosorbent assay (ELISA), respectively. ETAS showed significant increases in HSP70 mRNA at more than 0.125 mg/mL and the protein at more than 1.0 mg/mL. The antistress effect was evaluated in a murine sleep-deprivation model. A sleep-deprivation stress load resulted in elevation of blood corticosterone and lipid peroxide concentrations, while supplementation with ETAS at 200 and 1000 mg/kg body weight was associated with significantly reduced levels of both stress markers, which were in the normal range. The HSP70 protein expression level in mice subjected to sleep-deprivation stress and supplemented with ETAS was significantly enhanced in stomach, liver, and kidney, compared to ETAS-untreated mice. A preliminary and small-sized human study was conducted among healthy volunteers consuming up to 150 mg/d of ETAS daily for 7 d. The mRNA expression of HSP70 in peripheral leukocytes was significantly elevated at intakes of 100 or 150 mg/d, compared to their baseline levels. Since HSP70 is known to be a stress-related protein and its induction leads to cytoprotection, the present results suggest that ETAS might exert antistress effects under stressful conditions, resulting from enhancement of HSP70 expression.

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Section: Introductionsupporting

confidence: 62%

Enzyme‐Treated Asparagus Extract Promotes Expression of Heat Shock Protein and Exerts Antistress Effects

Ito

Maeda

Goto

et al. 2014

Journal of Food Science

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“…Furthermore, our group previously reported that ETAS improves cognitive impairment in senescence-accelerated mice [4]. In addition to in vivo study, it was also demonstrated that ETAS attenuates amyloid β-induced cellular injury in rat neuronal PC-12 cells in vitro [5].…”

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confidence: 87%

Enzyme-Treated Asparagus Extract Attenuates Hydrogen Peroxide-Induced Matrix Metalloproteinase-9 Expression in Murine Skin Fibroblast L929 Cells

Shirato

Takanari

Ogasawara

et al. 2016

Natural Product Communications

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Enzyme-treated asparagus extract (ETAS) exerts a wide variety of beneficial biological actions including facilitating anti-cortisol stress and neurological antiaging responses. However, the anti-skin aging effects of ETAS remain to be elucidated. Reactive oxygen species (ROS) play pivotal roles in skin aging. Increased ROS levels in fibroblasts in response to ultraviolet irradiation activate c-Jun N-terminal kinase (JNK) and its downstream transcription factor activator protein-1 (AP-1), and the resultant gene expression of matrix metalloproteinase (MMP) isoforms accelerates collagen breakdown in the dermis. Therefore, we explored whether ETAS has anti-skin aging effects by attenuating the oxidative stress responses in fibroblasts. Simultaneous treatment of murine skin L929 fibroblasts with hydrogen peroxide (H 2 O 2) and either ETAS or dextrin showed that ETAS significantly suppressed H 2 O 2-induced expression of MMP-9 mRNA as measured by real-time polymerase chain reaction. ETAS also clearly suppressed H 2 O 2-stimulated phosphorylation of c-Jun (AP-1 subunit) and JNK as determined by Western blot. However, ETAS did not affect the increased amounts of carbonyl proteins in response to H 2 O 2 , also as determined by Western blotting. These results suggest that ETAS diminishes cellular responsiveness to ROS but does not scavenge ROS. Thus, ETAS has the potential to prevent skin aging through attenuating the oxidative stress responses in dermal fibroblasts.

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“…Rodriguez et al (2005) and Sun et al (2007) emphasized the antioxidant activity of asparagus as it contains flavonoids and phenolic components. Sakurai et al (2013) showed that asparagus extract possesses a neuroprotective effect and attenuates cognitive impairment in senescence-accelerated mice.…”

Section: Introductionmentioning

confidence: 99%

Role of Matricaria recutita L. and Asparagus officinalis L. against the neurotoxicity of diazinon in rats

Elsaid

Shati

Sarhan

2015

The Journal of Basic & Applied Zoology

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Diazinon (DZN) is an organophosphorus insecticide widely used in agriculture. It has a variety of harmful effects on humans. Asparagus and chamomile have antioxidant properties and are used as antidotes of DZN in this study. Thirty-five adult male Sprague Dawley rats were divided into: control group; DZN group: subdivided into two subgroups which received 1 = 4 LD 50 and ½ LD 50 dose of DZN for 30 days; DZN and asparagus extract group: subdivided into two subgroups which received 1 = 4 LD 50 and ½ LD 50 dose of DZN respectively and treated with asparagus extract (300 mg/kg b. wt.) after 15 min of DZN administration; DZN and chamomile extract group: subdivided into two subgroups receiving DZN respectively and treated with chamomile extract (300 mg/kg b. wt.). The results herein showed that the antioxidant enzyme changes associated with the exposure to DZN are dose dependant in cerebrum, cerebellum and spinal cord tissues. The tumorigenicity of DZN was represented by the significant increase of arginase and the alpha-L-fucosidase in sera of all DZN groups. In addition, the molecular changes were investigated by the changes in Cu/Zn-dependent superoxide dismutase, glutathione-S-transferase and glutathione peroxidase enzymes that were altered after administration of DZN to rats. Present findings suggest that oral administration of aqueous extracts of asparagus or chamomile is able to restore the total antioxidant capacity, as demonstrated by the increase of superoxide dismutase activity, glutathione content and their relative enzymes in the investigated tissues. Due to their antioxidant activities, asparagus and chamomile are potential candidates as anti-neurotoxic agents. ª 2015 The Egyptian German Society for Zoology. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Enzyme-treated Asparagus officinalis Extract Shows Neuroprotective Effects and Attenuates Cognitive Impairment in Senescence-accelerated Mice

Cited by 20 publications

References 43 publications

Enzyme‐Treated Asparagus Extract Promotes Expression of Heat Shock Protein and Exerts Antistress Effects

Enzyme‐Treated Asparagus Extract Promotes Expression of Heat Shock Protein and Exerts Antistress Effects

Enzyme-Treated Asparagus Extract Attenuates Hydrogen Peroxide-Induced Matrix Metalloproteinase-9 Expression in Murine Skin Fibroblast L929 Cells

Role of Matricaria recutita L. and Asparagus officinalis L. against the neurotoxicity of diazinon in rats

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