Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Carlsson, Nils; Gustafsson, Hanna; Thörn, Christian; Olsson, Lisbeth; Holmberg, Krister; Åkerman, Björn

doi:10.1016/j.cis.2013.08.010

Cited by 222 publications

(182 citation statements)

References 166 publications

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“…Mesoporous silicon, due to its weak interactions with proteins, may be used as possible carrier for therapeutic protein delivery, 28 while other forms of silica support, such as sol-gel composites or mesoporous silica, due to strong protein adsorption may be used for enzyme immobilization and biocatalysis purposes. 29,30 In this paper we employ a fully atomistic molecular dynamics (MD) approach alongside Multi-Parametric Surface Plasmon Resonance (MP-SPR), contact angle and zeta potential measurements to further understand the details of how a model protein (hen egg white lysozyme, HEWL) adsorbs onto a SiO 2 surface.…”

Section: Introductionmentioning

confidence: 99%

Lysozyme adsorption at a silica surface using simulation and experiment: effects of pH on protein layer structure

Kubiak-Ossowska

Cwieka

Kaczyńska

et al. 2015

Phys. Chem. Chem. Phys.

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a Hen Egg White Lysozyme (HEWL) is a widely used exemplar to study protein adsorption on surfaces and interfaces. Here we use fully atomistic Molecular Dynamics (MD) simulations, Multi-Parametric Surface Plasmon Resonance (MP-SPR), contact angle and zeta potential measurements to study HEWL adsorption at a silica surface. The simulations provide a detailed description of the adsorption mechanism and indicate that at pH7 the main adsorption driving force is electrostatics, supplemented by weaker hydrophobic forces. Moreover, they reveal the preferred orientation of the adsorbed protein and show that its structure is only slightly altered at the interface with the surface. This provides the basis for interpreting the experimental results, which indicate the surface adsorbs a close-packed monolayer at about pH10 where the surface has a large negative zeta potential and the HEWL is positively charged. At higher pH, the adsorption amount of the protein layer is greatly reduced due to the loss of charge on the protein. At lower pH, the smaller zeta potential of the surface leads to lower HEWL adsorption. These interpretations are complemented by the contact angle measurements that show how the hydrophobicity of the surface is greatest when the surface coverage is highest. The simulations provide details of the hydrophobic residues exposed to solution by the adsorbed HEWL, completing the picture of the protein layer structure.

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Section: Introductionmentioning

confidence: 99%

Lysozyme adsorption at a silica surface using simulation and experiment: effects of pH on protein layer structure

Kubiak-Ossowska

Cwieka

Kaczyńska

et al. 2015

Phys. Chem. Chem. Phys.

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“…This contribution aims to cover the synthesis, functionalization, novel designs and other technological matters for MSP, more than their cellular or tissue interactions with living organisms. Excellent reviews about recent advances for mesoporous nanoparticles in biomedicine are published elsewhere [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Colloidal and spherical mesoporous silica particles: synthesis and new technologies for delivery applications

Beltrán-Osuna

Perilla

2015

J Sol-Gel Sci Technol

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Continuous research in prominent fields such as biotechnology, biomedicine and nanopharmaceutics has brought the development of a widespread class of materials, and studies for mesoporous materials have been exponentially growing lately. The purpose of this review is to provide a useful guide for different materials, methods and configurations that have been reported in the last 5 years for the synthesis of spherical mesoporous silica particles (MSP), in the colloidal size range (1-1000 nm). MSP exhibit several limitations that must be overcome in order to enable their medical and clinical use. Surface modification of these particles will allow getting new promising characteristics of these materials, including better drug release control and biocompatibility improvement. These modified MSP could be potentially used in many biomedical applications, especially for drug delivery systems. Emphasis is made on the pore size, diameter and shape of the final particles since these parameters will establish key characteristics, i.e., drug delivery profile, loading capacity and efficiency. Graphical Abstract Spreading the use of mesoporous silica particles in biomedicine is possible by the improvement of its inner characteristics through surface modification. This may be done by chemical functionalization or by coating with macromolecular layers or brushes, thus creating novel responsive core-shell hybrid composites to be used as carriers for drug delivery applications.

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“…Immobilized enzymes sometimes also tolerate more extreme pH, elevated temperature and higher salt concentration than the same protein in bulk solution 5 .…”

Section: Introductionmentioning

confidence: 99%

“…This technique is used to determine the amount of encapsulated protein (reviewed in 5 ), and sometimes also to monitor the kinetics of the immobilization 6 . A drawback often inherent to such indirect methods is high uncertainty and low sensitivity, and another limitation is the low time resolution that results from the time-consuming protocols for measuring protein concentration in bulk solution such as the Bradford method 7 .…”

Section: Introductionmentioning

confidence: 99%

A fluorescence spectroscopy assay for real-time monitoring of enzyme immobilization into mesoporous silica particles

Zadeh

Mallak

Carlsson

et al. 2015

Analytical Biochemistry

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Enzymes immobilized in mesoporous silica: A physical–chemical perspective

Cited by 222 publications

References 166 publications

Lysozyme adsorption at a silica surface using simulation and experiment: effects of pH on protein layer structure

Lysozyme adsorption at a silica surface using simulation and experiment: effects of pH on protein layer structure

Colloidal and spherical mesoporous silica particles: synthesis and new technologies for delivery applications

A fluorescence spectroscopy assay for real-time monitoring of enzyme immobilization into mesoporous silica particles

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