Enzymes immobilized on alumina and stainless steel supports

Abstract

SummaryEnzymes can be sorbed on inorganic carriers followed by crosslinking with glutaraldehyde. I n some cases superior results are obtained when the support is precoated with a porous layer of titanium oxide. Immobilized lactase prepared in this way retains essentially all its activity when stored under water a t 23°C for long periods of time and loses activity only slowly while treating cheese whey a t 55°C over the course of several weeks contact time. Furthermore, catalyst activity is unaffected by frequent sanitization. Optimum p H for these immobilized-lactase catalysts (enzyme produced from A . niger) is about 3.0 and optimum temperature is about 60°C. Amyloglucosidase catalase, 1.-asparaginase, and trypsin have also been immobilized by these techniques.

Kinetic and microstructural characterization of immobilized penicillin acylase fromStreptomyces lavendulaeon Sepabeads EC-EP

“…In the final state (E d ), the enzyme has lost all its activity. Such a deactivation pattern has been reported for several enzymes such as glucoamylase immobilized on collagen (Brillouet et al 1977), b-galactosidase immobilized on alumina (Charles et al 1975) and b-fructofuranosidase immobilized on porous glass (Thornton et al 1975). In our case, a grace period of time (t g ) was also observed at 408C in which the soluble and immobilized SlPVA apparently suffered no loss of enzymatic activity.…”

Scaling Up an Immobilized Enzyme System

“…In practical terms, this usually means maximizing the total product that can be produced by a given amount of immobilized enzyme composite. The total production P of a reactor during time tp can be related to the feed rate F by the equation: rtp Pt J Fdt (10) For the most common case, operation at constant conversion with exponential decay gives rtp p= fFe-(Wn2)tltindt (11) where Fj is initial feed rate and tj/2 is the immobilized enzyme halflife. When this expression is integrated, one obtains P = Fit1,2[1 e-(In2)tp/t/2] (12) Such isothermal operation at constant conversion requires decreasing flow rate to maintain the conversion level as enzyme activity falls.…”

Zeolite‐Coated Porous Arrays: A Novel Strategy for Enzyme Encapsulation

“…In addition, they possess high surface area as well as high mechanical, chemical, and thermal stability. The application of structured supports, such as ceramic materials, alumina and stainless steel supports for enzyme immobilization has been reported initially a few decades ago. Since then, numerous articles have been published on the immobilization of different types of enzymes using structured inorganic solids, such as ceramic supports, porous alumina or hybrid membranes, and stainless steels supports .…”