Enzymic Activity of Purified Plasma Membranes from the Yeast and Mycelial forms of Candida albicans

Marriott, M. S.

doi:10.1099/00221287-89-2-345

Cited by 40 publications

(12 citation statements)

References 13 publications

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“…5'-Nucleotidase (EC 3.1.3.5) has been widely used as a convenient and reliable marker for animal plasma membranes. However, reports suggest that it is not a suitable plasma membrane marker in fungal systems (Scarborough, 1975;Marriott, 1975). The results obtained in this study show that its activity is mostly in soluble form and that the low levels associated with particulate fractions are not associated with the plasma membrane.…”

Section: Discussionsupporting

confidence: 61%

Preparation of right-side-out plasma membrane vesicles from Penicillium cyclopium: a critical assessment of markers

Ugalde

Hernández²,

Galindo³

et al. 1992

Journal of General Microbiology

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A plasma membrane fraction was obtained by the combined use of differential centrifugation and aqueous polymer two-phase partitioning techniques. Vanadate-inhibited ATPase and glucan synthase activities were highly enriched in this fraction, although the presence of ATPase activity which was not inhibited by vanadate, nitrate, molybdate, anyimycin A or azide was also detected. Other intracellular membrane marker activities were present at very low or undetectable levels. A further separation step using Percoll density gradient Centrifugation resulted in the separation of a fraction which exclusively contained vanadate-inhibited ATPase activity, and was enriched with silicotungstic-acid-staining membrane material. Latency tests performed on the plasma membrane markers showed that the membrane vesicles were in the right-side-out orientation.

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Section: Discussionsupporting

confidence: 61%

Preparation of right-side-out plasma membrane vesicles from Penicillium cyclopium: a critical assessment of markers

Ugalde

Hernández²,

Galindo³

et al. 1992

Journal of General Microbiology

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“…Enzymatic activity measurements. The NADH dehydrogenase activity was measured by a modified version of the procedure originally decribed by Mariott (14). Typically, groups of 20 unirradiated or irradiated flies were homogenized in 3 mL of phosphate-buffered saline (PBS); the suspension was centrifuged at 700 g for 15 min, the pellet was collected, resuspended in PBS (1 mL) and sonicated for 3 min.…”

Section: Methodsmentioning

confidence: 99%

Symposium-in-Print Porphyrins and Related Compounds as Photoactivatable Insecticides. 3. Laboratory and Field Studies

Amor

Bortolotto

Jori

2000

Photochem Photobiol

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The exposure of populations of Ceratitis capitata (fruit fly), Bactrocera oleae (olive fly) and Stomoxis calcitrans (house fly) to a bait containing mumolar concentrations of porphyrin-type photosensitizers resulted in a significant accumulation of the porphyrin by the insects and a consequent development of photosensitivity upon exposure to visible light. The photoinsecticidal activity appeared to increase with increasing hydrophobicity of the porphyrin molecule: thus, the amphiphilic dicationic meso-di(cis-4N-methyl-pyridyl)-cis-diphenyl-porphine (n-octanol/water partition coefficient = 20) was markedly more efficient than its tricationic analogue or the dianionic hematoporphyrin (n-octanol/water partition coefficient = 12). The observed large decrease in the acetylcholinesterase activity of the photosensitized flies suggests that the damage of the nervous system gives an important contribution to the phototoxic action of porphyrins. Studies with C. capitata indicate that the photoinsecticidal action of porphyrins can be utilized to control the population of noxious insects also in open field conditions.

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“…3.6.1.3) and cytochrome c oxidase activity (E.C. 1.9.3.1) were measured spectrophotometrically as described by Marriot (1975) and Glaser and Hofer (1986), respectively.…”

Section: Methodsmentioning

confidence: 99%

Role of Specific Cellular Targets in the Hematoporphyrin‐sensitized Photoinactivation of Microbial Cells

Bertoloni

Zambotto

Conventi

et al. 1987

Photochem & Photobiology

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The hematoporphyrin‐sensitized photoinactivation of Candida albicans is mainly carried out by porphyrin molecules which are present in the cell‐incubation medium. Photoexcitation of hematoporphyrin by visible light appears to cause limited biochemical and functional alterations of the cytoplasmic membrane; this allows the penetration of the dye into the cell, with a consequent important photoinactivation of the mitochondria. The latter process is of critical importance for the decrease in the survival of the irradiated cells.

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Enzymic Activity of Purified Plasma Membranes from the Yeast and Mycelial forms of Candida albicans

Cited by 40 publications

References 13 publications

Preparation of right-side-out plasma membrane vesicles from Penicillium cyclopium: a critical assessment of markers

Preparation of right-side-out plasma membrane vesicles from Penicillium cyclopium: a critical assessment of markers

Symposium-in-Print Porphyrins and Related Compounds as Photoactivatable Insecticides. 3. Laboratory and Field Studies

Role of Specific Cellular Targets in the Hematoporphyrin‐sensitized Photoinactivation of Microbial Cells

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