Enzymic determination of plasma cholesterol on discrete automatic analysers

Nobbs, B.T.; Smith, Janet; Walker, Aaron

doi:10.1016/0009-8981(77)90434-x

Cited by 10 publications

(3 citation statements)

References 6 publications

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“…Fasting serum total cholesterol and triglyceride were measured enzymatically using automated methods. 15,16 Intra-assay coefficients of variation (CVs) for total cholesterol and triglyceride were 1.8 % and 1.2 %, respectively; and the inter-assay CVs were 3.5 % and 2.5 %, respectively. Hypercholesterolaemia was defined if serum cholesterol level was Ն5.18 mmol l −1 , 17 and hypertriglyceridaemia was defined if serum triglyceride level was Ն2.26 mmol l −1 .…”

Section: Methodsmentioning

confidence: 99%

A population-based study of the prevalence and associated factors of diabetes mellitus in southern Taiwan

Yang

et al. 1998

Diabet. Med.

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Diabetes mellitus is one of the major health care problems in Taiwan, since the mortality rate has increased from 7.91 per 100,000 in 1980 to 35.1 per 100,000 in 1996. To determine the prevalence of diabetes in southern Taiwan and to investigate possible associated factors, a stratified systematic cluster sampling of 1638 subjects (780 men and 858 women) aged > or =20 years living in Tainan city was investigated with a standard 75-g oral glucose tolerance test. The crude prevalence of diabetes in Tainan was 9.0% (10.3% men and 7.9% women) and the age-adjusted prevalence was 9.2% (10.4% men and 8.1% women). The crude prevalence of IGT was 14.0% (13.8% men and 14.1% women), and the age-adjusted prevalence was 15.5% (15.0% men and 15.9% women). The prevalence of diabetes by using the revised new diagnostic criteria was 7.5%. The prevalence of diabetes and IGT increased significantly with age for both genders, although the rises in prevalence of IGT in women was less consistent. Diabetic and IGT subjects were older and had higher levels of BMI, triglyceride, systolic and diastolic blood pressure, and higher prevalence of obesity, hypertension, and dyslipidemia but indulged in less physical activity than non-diabetic subjects. The significant factors associated with the newly diagnosed diabetes were age, family history of DM, BMI, systolic blood pressure, physical activity, and serum triglyceride levels.

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Section: Methodsmentioning

confidence: 99%

A population-based study of the prevalence and associated factors of diabetes mellitus in southern Taiwan

Yang

et al. 1998

Diabet. Med.

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“…The reagents were manufactured by E. Merck, Darmstadt, Federal Republic of Germany and supplied by BDH Chemicals, Peele, U.K. Comparative studies were carried out using the Vickers M300 method described above [11].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a programmable analyser – the Vitatron PA800

Smith

Fry

Walker

1981

Journal of Analytical Methods in Chemistry

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“…Trinder's reaction cholesterol. The results are discussed with regard to the is now the most commonly used; it was applied to difficulties resulting from the lack of a primary cholesterol assays by Allain et al (2) and adapted to cho iesterol standard for enzymic assay of cholesterol, many autoanalyzers -continuous-flow (3,4,5), centrifugal (6,7,8), the Vickers (9,10,11), and the Greiner GSA II (12).…”

Section: Introductionmentioning

confidence: 99%

Enzymic Assay of Plasma Cholesterol: A Comparison of Analytical Variations Found Using the Greiner GSA II and the Technicon SMA 12/60 and SMA II

Steinmetz¹,

E²,

Gaspart³

1979

Clinical Chemistry and Laboratory Medicine

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Summary: Cholesterol was assayed on the Greiner GSA II and the Technicon SMA 12/60 and SMA II autoanalyzers, vnthAllairfs entirely enzymic method (Allain et al. (1974), Clin. Chem. 20,470-475). The coefficients of variation in the results over a period of one month were less than 6% with the GSA II and the SMA II, but up to 9% on the SMA 12/60. Although the accuracy of the enzymic method depends on the specificity of the cholesterol oxidase and cholesterol esterase, the results were in good agreement with those obtained byAbelPs method (Abell et al. (1952) Allain et al. ((1974), Clin. Chem. 20, 470-475) bestimmt. Die Variationskoeffizienten der ber einen Zeitraum von einem Monat erhaltenen Ergebnisse waren < 6% mit dem GSA II und dem SMA II, aber bis zu 9% mit dem SMA 12/60. Obwohl die Richtigkeit der enzymatischen Methode von der Spezifit t der Cholesterinoxidase und -esterase abh ngt, stimmen die Ergebnisse gut mit denen berein, die mit der Methode von Abell et al. ((1952), J. Biol. Chem. 795, 357-366) an frischem oder lyophilisiertem Plasma von Menschen erhalten wurden. Die Cholestefinkonzentration war bei 4 °C oder in gefrorenem oder lyophilisiertem Plasma stabil. Keine der gepr ften Pharmaka st rten den enzymatischen Test.

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Enzymic determination of plasma cholesterol on discrete automatic analysers

Cited by 10 publications

References 6 publications

A population-based study of the prevalence and associated factors of diabetes mellitus in southern Taiwan

A population-based study of the prevalence and associated factors of diabetes mellitus in southern Taiwan

Evaluation of a programmable analyser – the Vitatron PA800

Enzymic Assay of Plasma Cholesterol: A Comparison of Analytical Variations Found Using the Greiner GSA II and the Technicon SMA 12/60 and SMA II

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