Enzymic synthesis of cerebroside from ceramide and uridine diphosphate galactose

Fujino, Yuichi; Nakano, Masahiro

doi:10.1042/bj1130573

Cited by 26 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We made several attempts at reproducing these results with synthetic stearoyl or lignoceroyl sphingosine as substrate, and using incubation conditions identical to those reported (27). We confirmed the increased cerebroside formation on addition of emulsified NFA-ceramide, and showed that the product was indeed NFA-cerebroside, but a control with Tween 20 (one of the detergents in the emulsion) yielded a similar effect.…”

Section: Discussionsupporting

confidence: 66%

“…Fujino and Nakano (27) have recently published a note on the biosynthesis of cerebroside from ceramide by rat brain microsomes, but, it is not clear whether the biosynthetic cerebroside was of the hydroxy or nonhydroxy type. Moreover, it was not determined whether the biosynthetic cerebroside was actually derived from the exogenous ceramide mixture or whether the exogenous ceramide-detergent mixture simply stimulated the galactosylation of endogenous material.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty acids

Morell

Costantino-Ceccarini

Radin

1970

Archives of Biochemistry and Biophysics

109

View full text Add to dashboard Cite

Incubation of microsomes from mouse brain with UDP-gal, a long-chain base, and W-fatty acyl-CoA resulted in formation of NFA-cerebroside labeled in the fatty acid residue. The rate of synthesis was ten times that of the system lacking UDP-gal or long chain base. The incubation system also formed NFA-ceramide, at a much higher rate. Stearoyl-and lignoceroyl-CoA were much more effective in forming ceramide and cerebroside than palmitoyl-and oleoyl-CoA, a correlation which suggests that ceramide is an intermediate in cerebroside biosynthesis. When NFA-ceramide, together with a phospholipid mixture, was incubated wit,h labeled UDP-gal, NFA-cerebroside was formed. Utilization of the exogenous ceramide was demonstrated by a number of chromatographic procedures. Under the same conditions, but with labeled UDP-glc, the corresponding glucose-containing cerebroside was formed, at a somewhat higher rate. The organic synthesis of labeled lignoceric acid and lignoceroyl n-sphingosine is described. Also described is a simplified method for large-scale preparation of purified n-sphingosine. The labeled ceramide was utilized by the microsomes for the formation of galactosyl lignoceroyl sphingosine. Under a variety of conditions the microsomes failed to convert psychosine and labeled acyl-CoA-to cerebroside.

show abstract

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%