Silver nanoparticles (AgNPs) ranging in size from 1-100 nm show good application potential in many medical fields (therapies, medical devices, molecular diagnostics) due to their antimicrobial properties. The purpose of this study is to characterize from physicochemical perspective the colloidal dispersion obtained through phytosynthesis. The existence of colloidal silver particles was visually highlighted through Thyndall effect. The bioreduction of silver ions was analyzed through modern techniques, UV-VIS spectrophotometry and Hyperspectral Microscopy. After getting the colloidal dispersion, its antibacterial activity was proved by sowing on different plates the following types of pathogenic agents: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The UV-VIS spectrum for the methanolic extract with 10% H. perforatum L. and for the silver colloidal dispersions was achieved, observing a max at 455 nm. The hyperspectral images were achieved observing the shape, conformation, and the size of the obtained particles. During the antibacterial efficacy testing on those three strains of pathological agents, in all situations, the colloidal dispersion had a promising antimicrobial effect.The term nanotechnology was used for the first time by Norio Taniguchi in 1974, and represents the set of technologies and researches, physical, chemical and biological at the scale of particles with size smaller than 100 nm [1][2][3]. Recently, the nanoparticles from noble metals have been the target of many researches concerning their unique electronic, optical, mechanical, magnetic and chemical properties. Their specific properties are different due to their dimension and the size of their specific surface [4][5][6]. They have applications in different fields: catalysing some reactions, in the electronic field, in photonics, in photography, in textile industry [7,8], in cosmetics industry [9], in dye industry, technical-medical products [10,11], in the diagnosis field [12], in the treatment of different acute and chronical severe diseases like malaria, hepatitis, cancer [13][14][15][16] and AIDS, and as vectors for some medicines [17,18].The antibacterial effect of silver ions has been known for a long time: in concentrations of 1:20.000.000 affect the thiol groups, precipitating the proteins and interfering the vital enzymatic processes of bacterial cells. But the use of AgNPs as antibacterial agent is relatively new. In comparison with the silver ions, the antimicrobial activity of the colloidal silver particles is influenced by the size of the particles, their size being inversely proportional with the antimicrobial effect, due to their increased reactivity determined by the big ratio surface to volume [19].A few preparation methods for the metallic nanoparticles were reported: reduction of some salts, radiolysis, electrochemical synthesis, laser ablation and thermal ablation. In order to get the AgNPs, the simplest method is the reduction of some silver salts -they produced nanoparticles with different morphologi...