Eosinophilic Granulated Cells Comprising a Tumor in a Fischer Rat

Miyajima, Rumiko; Hosoi, Masayo; Yamamoto, Shinji; Mikami, Shinichi; Yamakawa, Seiki; Iwata, Hijiri; Enomoto, Makoto

doi:10.1177/019262339902700210

Cited by 5 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A tumor composed of cells with eosinophilic cytoplasmic granules that was observed recently in an F344 rat might have originated from GLs. 13 The cellular morphology and other characteristics of the present tumors are very similar to those reported previously. 13 However, the derivation of GLs appears definitely to differ between rodents and cats; those in rodents are mast cells, 6,7,14 and those in cats are LGLs.…”

supporting

confidence: 88%

“…Since the first feline case described in 1972, 3 several similar feline cases have been reported and are referred to as globule leukocyte (GL) tumors, 3,5,12 granulated round cell tumors, 11 or large granular lymphocytic (LGL) lymphomas. 4,9 Recently, the first case in rats was reported, 13 but its features and derivation were not fully discussed. A database search of long-term studies conducted at our laboratories since 1990 revealed three similar cases in Fischer 344 (F344) rats.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Spontaneous Eosinophilic Granulated Round Cell Tumors in Rats

Nagatani

Nakamura²,

Yamaguchi³

et al. 2001

Vet Pathol

View full text Add to dashboard Cite

Morphologic and histochemical characteristics were noted for three spontaneous tumors with eosinophilic cytoplasmic granules that occurred in aged Fischer 344 rats. Macroscopic lesions were widely distributed in the body, mainly involving the intra-abdominal adipose tissue, pancreas, and mesenterium. These lesions were generally hard swellings with nodular and sclerosing areas. Bloody ascites was a concomitant finding. Histologically, the tumor cells were round, from 9 to 30 microm in diameter with one or two round to oval nuclei, and characterized by eosinophilic granules (0.5-2.0 microm) that stained definitely to weakly positive with the periodic acid-Schiff reaction and demonstrated no metachromasia with toluidine blue stain. Furthermore, the granules were characterized by a positive reaction with lectin histochemistry for concanavalin A (Con A), wheat germ agglutinin (WGA), phaseolus vulgaris agglutinin (PHA-E4), lens culinaris agglutinin (LCA), and recinus communis agglutinin (RCA-I) in all tumors and for ulex europaeus agglutinin (UEA-I), peanut agglutinin (PNA), and soybean agglutinin (SBA) in one tumor. Positive reactions for anti-rat mast cell protease II and CD8 were not demonstrated immunohistochemically. Abundant glycogen was noted in the large tumor cells from one rat. With electron microscopy, the cytoplasmic granules were identified as electron-dense homogenous bodies bounded by a single unit membrane. These characteristics are similar to those of granulated metrial gland cells, but further study is needed to clarify the cell of origin for these tumors.

show abstract

supporting

confidence: 88%

mentioning

confidence: 99%

Spontaneous Eosinophilic Granulated Round Cell Tumors in Rats

Nagatani

Nakamura²,

Yamaguchi³

et al. 2001

Vet Pathol

View full text Add to dashboard Cite

show abstract

“…The ultrastructural features of granules resembled eosinophilic granulated cells comprising a tumor in a Fischer rat in a previous report of Miyajima et al. 12 . They reported that the granules partially showed a filament-like structure.…”

supporting

confidence: 54%

“…Various histochemical and immunohistochemical stains and electron microscopic examination revealed that this neoplasm was a globule leukocyte tumor. While eosinophilic granulated round cell tumors 11 and an eosinophilic granulated cell tumor 12 with similar granules were previously reported in rats, the origin of these granulated cells was not clear. To our knowledge, no report has yet described a diagnosis of globule leukocyte tumor in rats.…”

mentioning

confidence: 60%

Spontaneous globule leukocyte tumor accompanied by inflammatory cells in a Wistar Hannover rat

Sato¹,

Tomonari²,

Doi³

et al. 2019

J Toxicol Pathol

View full text Add to dashboard Cite

We encountered hematolymphoid neoplastic lesions in the form of many nodules in the spleen and liver in a 110-week-old male Wistar Hannover rat (Crl:WI (Han)). The lesions contained atypical proliferative cells, eosinophils, lymphocytes, and macrophages. The proliferative cells comprised various atypical cell types with or without cytoplasmic eosinophilic granules. The granules were positively stained using periodic acid-Schiff and elastase stains, were bluish purple using phosphotungstic acid and hematoxylin, and showed no metachromasia using toluidine blue. In immunohistochemical staining, the proliferative cells with or without granules were positive for granzyme B, rat mast cell protease II, and Ki67. Electron microscopic examination revealed that single to multiple high-density granules of variable size were covered by a membrane. These findings led to a diagnosis of globule leukocyte tumor. The accompaniment of this tumor by inflammatory cells is likely evoked by mast cell-like active mediators contained in the granules of the globule leukocytes.

show abstract

“…Granular cell tumors and globule leukocyte tumors are characterized as round cell tumors with eosinophilic granules, similar to those observed in the current case. Accordingly, metachromasia, using toluidine blue, was required to confirm the diagnosis 1 , 2 , 13 , 14 .…”

mentioning

confidence: 99%

Malignant mast cell tumor of the thymus in an Royal College of Surgeons (RCS) rat

2017

View full text Add to dashboard Cite

A 152-week-old male Royal College of Surgeons (RCS) rat kept as a non-treated animal in a long-term animal study presented with a soft mass in the anterior mediastinum, which adhered to the pleura of the lung. Histopathologically, the mass mainly consisted of round to short spindle-shaped tumor cells that had infiltrated through the hyperplastic thymic tissue. The tumor cells were arranged in loose to dense sheets. Nuclei were moderate in size and round to spindle-shaped, with small nucleoli. Almost all tumor cells exhibited abundant eosinophilic cytoplasm, including eosinophilic granules of a range of sizes. The granules of tumor cells exhibited metachromasia with toluidine blue stain and were positive for c-kit and mast cell protease II. These findings indicate that the tumor described here represents a rare case of spontaneous malignant mast cell tumor with thymic epithelial hyperplasia.

show abstract

Eosinophilic Granulated Cells Comprising a Tumor in a Fischer Rat

Cited by 5 publications

References 8 publications

Spontaneous Eosinophilic Granulated Round Cell Tumors in Rats

Spontaneous Eosinophilic Granulated Round Cell Tumors in Rats

Spontaneous globule leukocyte tumor accompanied by inflammatory cells in a Wistar Hannover rat

Malignant mast cell tumor of the thymus in an Royal College of Surgeons (RCS) rat

Contact Info

Product

Resources

About