Eosinophils, mast cells, nerves and ganglion cells in appendicitis

Abstract

Introduction Twenty to thirty percent appendices removed from patients with suspected appendicitis appear normal on histology. The cause of pain in these patients is unknown. The presence of eosinophils and mast cells should be looked at skeptically which may explain the cause of pain. The aim was to study the eosinophils, mast cells, nerves and ganglions in normal and infl amed appendices.Materials and methods A total of 329 appendices including 192 case of acute appendicitis (group A), 94 cases of clinically acute but histologically normal appendices (group B), 13 cases of complementary/elective appendicectomies (group C) and 30 normal controls from medico-legal autopsies(group D), were studied for the presence of eosinophils, mast cells, nerves and ganglia in mucosa, submucosa and muscularis propria. Routine haematoxylin and eosin stained sections were used for eosinophils, nerves and ganglia and Toludine blue sections for mast cell counts. One way ANOVA and logistic regression was used for statistical analysis ResultsThe mean eosinophil and mast cell counts were signifi cantly higher in mucosa, submucosa and muscularis propria in Group A and B, when compared to group C+D. The number of nerves and ganglion cells were signifi cantly higher in group A when compared to groups B and C+D. The correlation between eosinophil and mast cell count was not found to be statistically signifi cant.Conclusion A signifi cant increase in Eosinophils, mast cells, nerves and ganglion cells was seen in acute appendicitis. Increase in eosinophils and mast cells may explain the pain in histologicaly normal but clinically suspected acute appendicitis.

The effect of vitamin E on mast cells in small intestine of broilers under heat stress

“…For that reason, it was stated that it may be contributed to the formation of epithelial damage in the small intestine of laying hens (Deng et al, 2012). Also, it was determined that the number of mast cells increased in the diseases such as appendicitis (Coskun et al, 2003;Singh et al, 2008), inflammatory bowel disease (Goral et al, 2010), mastocytes enterocolitis (Jakate et al, 2006) and ulcerative colitis (Stasikowsk A-Kanick et al, 2012;Zhao et al, 2009;Magro et al, 2006). In the present study, the number of mast cells was significantly higher in duodenum at the fifth week compare to the fourth week in vitamin E+heat stress group.…”

Morphology and Serum Ige Levels in Recurrent Acute Appendicitis.

“…Acute appendicitis with eosinophilic infiltration was mentioned as eosinophilic gastroenteritis rather than a variant of appendicitis (Jona et al, 1976). Eosinophil infiltration of muscle layer in acute appendicitis was reported as a widespread phenomenon in few studies (Aravindan, 1997;Singh et al, 2008). The presence of acute eosinophilic appendicitis is suggested as an early marker of acute appendicitis (Rajeswari et al, 2015).…”

Is there a role of interstitial cells of Cajal and mast cells and eosinophilsin appendicitis in children?

“…However, there are many examples of appendices that are removed during surgery because of suspected appendicitis in which histological evidence of appendicitis is absent. ICC, MCs, and eosinophils could be cell populations responsible for pain in clinically suspected acute appendicitis (3,4).…”

“…The number of eosinophils and mast cells (MCs) may change during acute and chronic inflammatory conditions. In appendicitis, levels of eosinophil and MCs are not clearly defined (4). ICC, MCs, and eosinophils may play an important role in appendicitis pathogenesis.…”