EphB6 overexpression and Apc mutation together promote colorectal cancer

Abstract

The erythropoietin-producing hepatocyte (Eph) family tyrosine kinases play important roles in tumorigenesis and cancer aggression. In this study, we investigated the role of EphB6 in oncogenic transformation of colorectal epithelial cells in vitro and in vivo. EphB6 is upregulated in human colorectal cancer (CRC) tissues as compared to normal tissues, and its overexpression promotes proliferation, migration and invasion by IMCE colorectal adenoma cells, in which one Apc allele is mutated. EphB6 overexpression together with Apc mutation leads to the development of colorectal tumors in vivo. Expression microarrays using mRNAs and lncRNAs isolated from EphB6-overexpresssing IMCE and control cells revealed a large number of dysregulated genes involved in cancer-related functions and pathways. The present study is the first to demonstrate that EphB6 overexpression together with Apc gene mutations may enhance proliferation, invasion and metastasis by colorectal epithelial cells. Microarray data and pathway analysis of differentially expressed genes provided insight into possible EphB6-regulated mechanisms promoting tumorigenesis and cancer progression. EphB6 overexpression may represent a novel, effective biomarker predictive of cell proliferation, invasion and metastasis patterns in CRC tumors.

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

“…Furthermore, miR‐497‐5p was found reduced expression and its overexpression was found to suppress NSCLC cell growth via targeting FGF2 . EPHB6, belong to the Eph receptors family, that was found elevated expression in colorectal cancer and its overexpression accelerates colorectal cancer metastasis . Furthermore, reduced EPHB6 expression was found in gastric carcinoma and associated with malignancy indicators for cancer .…”

“…19 EPHB6, belong to the Eph receptors family, that was found elevated expression in colorectal cancer and its overexpression accelerates colorectal cancer metastasis. 20 Furthermore, reduced EPHB6 expression was found in gastric carcinoma and associated with malignancy indicators for cancer. 21 In this work, we found DGCR5 and EPHB6 shared the same binding for acted as ceRNA to regulate miR-211-5p in NSCLC.…”

The design of novel inhibitors for treating cancer by targeting CDC25B through disruption of CDC25B-CDK2/Cyclin A interaction using computational approaches

“…Cancers of lung (19.4% of the total), liver (9.1%), and stomach (8.8%) processed the highest mortality, which are caused by disordered cell cycle and irregularities such as deletions, over-expression, or mutations in the molecules [2–4]. Therefore, the urgent need for identifying novel and efficient agents to cure the cancer should be paid more attentions.…”

Improving prediction performance of colon cancer prognosis based on the integration of clinical and multi-omics data

“…In total, 57 differentially expressed genes were identified, as listed in Additional file 3. These included PRSS2, EPHB6 and FABP4, which showed correlation with colorectal cancer prognosis, whereas high expression of these genes was related to poor prognosis [41][42][43]. These genes were enriched in Reactome pathway EPH-ephrin mediated repulsion of cells, which might be a potential therapeutic target in colon cancer [44,45].…”