EPIC markers of the genus Apis as diagnostic tools for detection of honey fraud

Moškrič, Ajda; Mole, Katarina; Prešern, Janez

doi:10.1016/j.foodcont.2020.107634

Cited by 11 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Honey DNA is a mixture of DNA from many different organisms, such as honey bees, plants, and microorganisms 28 , 29 and it can be used to authenticate the plant species that the honey bees foraged from 30 , 31 , distinguish the geographical origin of honey 32 , 33 , trace the entomological origin of honey 17 , 34 , and monitor the health of the honey bee colony 35 – 37 . The quality of DNA plays a significant role in verifying the authenticity of food products using molecular methods, and it can be influenced by several factors, such as the manufacturing process, extraction technique, and composition of the food matrix, among various other considerations 25 .…”

Section: Resultsmentioning

confidence: 99%

An innovative molecular approach towards the cost-effective entomological authentication of honey

Zhang,

Yuan

et al. 2024

npj Sci Food

View full text Add to dashboard Cite

Honey authentication and traceability are crucial not only for economic purposes but also for ensuring safety. However, the widespread adoption of cutting-edge technologies in practical applications has been hampered by complex, time-consuming sample pre-treatment processes, the need for skilled personnel, and substantial associated expenses. This study aimed to develop a simple and cost-effective molecular technique to verify the entomological source of honey. By utilizing newly designed primers, we successfully amplified the mitochondrial 16S ribosomal RNA gene of honey bees from honey, confirming the high quality of the extracted DNA. Employing RFLP analysis with AseI endonuclease, species-specific restriction patterns were generated for honey derived from six closely related honey bees of the Apis genus. Remarkably, this method was proven equally effective in identifying heat-treated and aged honey by presenting the same RFLP profiles as raw honey. As far as we know, this is the initial research of the simultaneous differentiation of honey from closely related honey bee species using the restriction endonuclease AseI and mitochondrial 16S rRNA gene fragments. As a result, it holds tremendous potential as a standardized guideline for regulatory agencies to ascertain the insect origins of honey and achieve comprehensive traceability.

show abstract

Section: Resultsmentioning

confidence: 99%

An innovative molecular approach towards the cost-effective entomological authentication of honey

Zhang,

Yuan

et al. 2024

npj Sci Food

View full text Add to dashboard Cite

show abstract

“…There are also some reports on the use of honeybee products as sources of DNA, which are another promising non-destructive source harboring immense DNA information. For example, using an appropriate set of primers, the entomological origin of honey can be determined to a certain degree, like distinguishing between species or, in some cases, even between some of the subspecies of honeybees [ 59 , 60 ]. While these sources do not seem directly applicable to queen breeding, genotyping of csd alleles using NGS technology yielded a diversity of these alleles within the colony [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Non-Destructive Genotyping of Honeybee Queens to Support Selection and Breeding

Bubnič

Mole

Prešern

et al. 2020

Insects

Self Cite

View full text Add to dashboard Cite

In traditional bee breeding, the honeybee queen is chosen for breeding based on the performance of the colony produced by its mother. However, we cannot be entirely certain that a specific queen will produce offspring with desirable traits until we observe the young queen’s new colony. Collecting the queen’s genetic material enables quick and reliable determination of the relevant information. We sampled exuviae, feces, and wingtips for DNA extraction to avoid fatally injuring the queen when using tissue samples. Quantity and purity of extracted DNA were measured. Two mitochondrial markers were used to determine the lineage affiliation and exclude possible contamination of DNA extracts with non-honeybee DNA. dCAPS (derived Cleaved Amplified Polymorphic Sequences) markers allowed detection of single nucleotide polymorphisms (SNPs) in nuclear DNA regions presumably associated with Varroa sensitive hygiene and set the example of successful development of genotyping protocol from non-destructive DNA sources. One of the logical future steps in honeybee breeding is introducing genomic selection and non-destructive sampling methods of genetic material may be the prerequisite for successful genotyping. Our results demonstrate that the extraction of DNA from feces and exuviae can be introduced into practice. The advantage of these two sources over wingtips is reducing the time window for processing the samples, thus enabling genotyping directly after the queen’s emergence.

show abstract

“…Soares et al exploits DNA barcoding combined with HRM analysis to differentiate the honey species in three clusters with confidence levels > 99%, being the results well correlated with the sequencing analysis ( Soares, et al, 2018 ). In a recent study, a novel DNA-based tool has been proposed for correct identification of DNA signatures in three economically important honeybee species (i.e., Apis mellifera , Apis cerana , and Apis dorsata ) ( Moškrič, Mole, & Prešern, 2021 ).…”

Section: Methods For Honey Authenticity Verificationmentioning

confidence: 99%

A comprehensive review of the current trends and recent advancements on the authenticity of honey

Zhang,

Gu,

Liu

et al. 2023

Food Chemistry: X

View full text Add to dashboard Cite

EPIC markers of the genus Apis as diagnostic tools for detection of honey fraud

Cited by 11 publications

References 47 publications

An innovative molecular approach towards the cost-effective entomological authentication of honey

An innovative molecular approach towards the cost-effective entomological authentication of honey

Non-Destructive Genotyping of Honeybee Queens to Support Selection and Breeding

A comprehensive review of the current trends and recent advancements on the authenticity of honey

Contact Info

Product

Resources

About