Epicatechin-3-gallate reverses TGF-β1-induced epithelial-to-mesenchymal transition and inhibits cell invasion and protease activities in human lung cancer cells

Huang, Steve S.; Horng, Chi‐Ting; Hsieh, Yih‐Shou; Hsieh, Yi‐Hsien; Chu, Shu‐Chen; Chen, Pei‐Ni

doi:10.1016/j.fct.2016.05.009

Cited by 40 publications

(36 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A compound derived from Dendrobium ellipsophyllum, bibenzyl 4,5,4′ -trihydroxy-3,3′-dimethoxybibenzyl was shown to inhibit EMT of lung cancer cells via downregulating EMT markers (vimentin and Snail) and upregulating E-cadherin [27]. Such EMT suppression was also observed in lung cancer cells treated with other single compounds obtained from Chinese medicine, such as moscatilin [28], gigantol [29], and epicatechin-3-gallate [30]. A flavonoid obtained from Stellera chamaejasme L., namely chamaejasmenin B, was also reported to block the TGF-β-induced EMT in breast cancer [31].…”

Section: Suppression Of Epithelial-mesenchymal Transitionmentioning

confidence: 71%

Recent Progress on the Molecular Mechanisms of Anti-invasive and Metastatic Chinese Medicines for Cancer Therapy

Wei¹,

Wang²,

Feng³

2017

Unique Aspects of Anti-Cancer Drug Development

View full text Add to dashboard Cite

Despite of the recent advances in diagnostic and therapeutic approaches, cancer remains as the leading cause of death worldly with diverse causal factors regarding genes and environment. Invasion and metastasis, as one of the most important hallmarks for cancer, have restrained the successful clinical therapy and are the primary causes of death among cancer patients. So far, most chemotherapeutic drugs are not effective for metastatic cancer due to drug resistance and serious side effects. Therefore, it is urgently essential to develop more effective therapeutic methods. Owing to their diverse biological activities and low toxicity, naturally active compounds derived from Chinese medicines, as a complementary and alternative approach, are reported to promote the therapeutic index and provoked as an excellent source for candidates of anti-metastatic drugs. With the rapid development of molecular biology techniques, the molecular mechanisms of the effects of potential anti-invasive and metastatic Chinese medicines are gradually elucidated. This chapter reviews the potential anti-invasive and metastatic mechanisms of naturally active compounds from Chinese medicines, including suppression of EMT, proteases and cancer-induced angiogenesis, anoikis regulation of circulating tumor cells and regulation of miRNA-mediated gene expression, providing scientific evidence for clinically using Chinese medicines in the field of cancer therapy.

show abstract

Section: Suppression Of Epithelial-mesenchymal Transitionmentioning

confidence: 71%

Recent Progress on the Molecular Mechanisms of Anti-invasive and Metastatic Chinese Medicines for Cancer Therapy

Wei¹,

Wang²,

Feng³

2017

Unique Aspects of Anti-Cancer Drug Development

View full text Add to dashboard Cite

show abstract

“…The invaded cells were fixed with methanol and stained with Giemsa. Cell numbers were counted using a light microscope (magnification 100×), while motility assay was conducted as described for the invasion assay, without Matrigel coating …”

Section: Methodsmentioning

confidence: 99%

“…Nonadherent cells were removed by washing the dishes with phosphate‐buffered saline. After staining with 0.1% crystal violet, fixed cells were lysed in 0.2% Triton X‐100 and the absorbance was measured at 550 nm …”

Section: Methodsmentioning

confidence: 99%

Cinnamomum cassia extracts reverses TGF-β1-induced epithelial-mesenchymal transition in human lung adenocarcinoma cells and suppresses tumor growth in vivo

Chen

Hsieh

Yang

et al. 2017

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

Metastasis is the most common cause of cancer-related mortality in patients, and epithelial-mesenchymal transition (EMT) is essential for cancer metastasis and antidrug resistance. Cinnamomum cassia has several antioxidative, anti-inflammatory, and anticancer biological effects. However, the anti-EMT effect of C. cassia in human lung carcinoma is rarely reported. In this study, we determined whether C. cassia extracts (CCE) reduces the EMT and tumor growth of human lung adenocarcinoma cells. CCE inhibited the transforming growth factor (TGF)-β1-induced cell motility and invasiveness of A549 and H1299 cells by repressing matrix metalloproteinase-2 and urokinase-type plasminogen activator as well as impaired cell adhesion to collagen. CCE also affected the TGF-β1-induced EMT by downregulating the expression of vimentin and fibronectin and upregulating E-cadherin. The nude mice xenograft model showed that CCE reduced A549 tumor growth. Thus, CCE possesses antimetastatic activity of A549 and H1299 cells by affecting EMT and suppressing A549 tumor growth in vivo. This result suggested that CCE could be used as an antimetastatic agent or as an adjuvant for anticancer therapy.

show abstract

“…Several natural flavonoids have been investigated as potential anti-metastasis agents toward various lung cancer cell lines. Epicatechin-3-gallate [16], a natural flavonoid found in green tea, was reported to reverse the TGF-β1-induced EMT and up-regulated epithelial markers, such as E-cadherin (64). Moreover, flavone components in Scutellaria baicalensis, including baicalein [17] and wogonin [18], induced down-regulation of MMP-2 and MMP-9 in both A549 and H1299 cells (65).…”

Section: Cancer Metastatic Process Metastasis Is a Complicated Multimentioning

confidence: 99%

Potential Anti-metastasis Natural Compounds for Lung Cancer

Chanvorachote

Chamni

Ninsontia

et al. 2016

View full text Add to dashboard Cite

Epicatechin-3-gallate reverses TGF-β1-induced epithelial-to-mesenchymal transition and inhibits cell invasion and protease activities in human lung cancer cells

Cited by 40 publications

References 36 publications

Recent Progress on the Molecular Mechanisms of Anti-invasive and Metastatic Chinese Medicines for Cancer Therapy

Recent Progress on the Molecular Mechanisms of Anti-invasive and Metastatic Chinese Medicines for Cancer Therapy

Cinnamomum cassia extracts reverses TGF-β1-induced epithelial-mesenchymal transition in human lung adenocarcinoma cells and suppresses tumor growth in vivo

Potential Anti-metastasis Natural Compounds for Lung Cancer

Contact Info

Product

Resources

About