(−)-Epicatechin Gallate (ECG) Stimulates Osteoblast Differentiation via Runt-related Transcription Factor 2 (RUNX2) and Transcriptional Coactivator with PDZ-binding Motif (TAZ)-mediated Transcriptional Activation

Byun, Mi Ran; Sung, Mi Kyung; Kim, A. Rum; Lee, Cham Han; Jang, Eun Jung; Jeong, Mi Gyeong; Noh, Minsoo; Hwang, Eun Sook; Hong, Jeong Ho

doi:10.1074/jbc.m113.522870

Cited by 46 publications

(48 citation statements)

References 44 publications

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“…Growth factor-mediated signaling pathways, such as Wnt and TGF-b, and transcriptional regulators TAZ and Runx2 have been shown to direct osteogenesis from precursor cells [32], but concurrently inhibit adipogenesis [33]. In addition, pro-osteogenic compounds, including resveratrol, Epicatechin gallate, and oxysterols, often exhibit anti-adipogenic activities [34][35][36]. In the present study, we show the ability of sulfuretin to promote osteoblast differentiation of preosteoblast MC3T3-E1, mesenchymal C3H10T1/2 cells, and primary bone marrow cells.…”

Section: Discussionmentioning

confidence: 98%

Sulfuretin induces osteoblast differentiation through activation of TGF-β signaling

et al. 2015

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The identification and examination of potential determinants controlling the progression of cell fate toward osteoblasts can be intriguing subjects. In this study, the effects of sulfuretin, a major compound isolated from Rhus verniciflua Stokes, on osteoblast differentiation were investigated. Treatments of sulfuretin induced alkaline phosphatase (ALP) activity in mesenchymal C3H10T1/2 cells and mineralization in preosteoblast MC3T3-E1 cells. Pro-osteogenic effects of sulfuretin were consistently observed in freshly isolated primary bone marrow cells. In mechanical studies, sulfuretin specifically induced expression of TGF-β target genes, such as SMAD7 and PAI-1, but not other signaling pathway-related genes. Similar to the results of gene expression analysis, reporter assays further demonstrated TGF-β-specific induction by sulfuretin. Furthermore, disruption of TGF-β signaling using treatment with TGF-β-specific inhibitor, SB-431542, and introduction of SMAD2/3 small interfering RNA impaired the effects of sulfuretin in inducing ALP activity and expression of ALP mRNA. Together, these data indicate that the pro-osteogenic effects of sulfuretin are mediated through activation of TGF-β signaling, further supporting the potential of sulfuretin in the prevention of bone-related diseases such as bone fracture and osteoporosis.

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Section: Discussionmentioning

confidence: 98%

Sulfuretin induces osteoblast differentiation through activation of TGF-β signaling

et al. 2015

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“…Plant extracts have been used to treat cancers due to their effective anticancer properties [14]. In addition to the anti-cancer effects, various plant extracts have also been studied for its proliferation and differentiation effects of stem cells [15][16][17]. Watercress with the botanical name Nasturtium officinale is a perennial European herb of the family Cruciferae (mustard family) that grows in freshwater streams.…”

Section: Introductionmentioning

confidence: 99%

Watercress-based electrospun nanofibrous scaffolds enhance proliferation and stemness preservation of human adipose-derived stem cells

Dadashpour

Pilehvar-Soltanahmadi

Mohammadi

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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The present study describes the effects of Watercress extract (WE) based electrospun nanofibrous mats on the regulation of adhesion, proliferation, cytoprotection and stemness preservation of adipose-derived stem cells (ADSCs). Watercress (Nasturtium officinale) is one of the most important medicinal plant with a board spectrum of biological functions. For this purpose, WE-loaded PCL-PEG nanofibers were fabricated by electrospinning and characterized using FE-SEM and FTIR. Adhesion, proliferation and cytoprotection of ADSCs on the nanofibers was investigated using FE-SEM and MTT assays. Analysis of cell cycle was carried out by flow-cytometry. Finally, qPCR was applied to measure the expression levels of cell cycle-regulated genes and stemness markers of ADSCs grown on the nanofibers. In this study, we found that WE-loaded PCL-PEG nanofibers had great antioxidant potential and exhibited higher cytoprotection, better adhesion, and significantly increased proliferation of ADSCs. The greater proliferation and preserving stemness ability of ADSCs on WE based nanofibers was further confirmed by higher expression levels of cell cycle-regulated genes and stemness markers. These results demonstrate that WE-loaded PCL-PEG electrospun nanofibrous mats appear suitable to support ADSCs adhesion and proliferation while concurrently preserving the cell stemness, therefore representing a hopeful approach for applying in stem cell based regenerative medicine.

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“…These terms were also found used in literature on the occurrence and development of OP. For example, RUNX2 has been reported to stimulate osteoblast differentiation (Byun et al, 2014). AMP-activated protein kinase (AMPK) affects bone loss, increasing the risk of osteoporotic fracture (Jeyabalan et al, 2012).…”

Section: Gene-level Analysismentioning

confidence: 99%

MicroRNA Alterations for Diagnosis, Prognosis, and Treatment of Osteoporosis: A Comprehensive Review and Computational Functional Survey

Zhang

et al. 2020

Front. Genet.

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Osteoporosis (OP) is a systemic bone disease with a series of clinical symptoms. The use of screening biomarkers in OP management is therefore of clinical significance, especially in the era of precision medicine and intelligent healthcare. MicroRNAs (miRNAs) are small, non-coding RNAs with the potential to regulate gene expression at the post-transcriptional level. Accumulating evidence indicates that miRNAs may serve as biomarkers for OP prediction and prevention. However, few studies have emphasized the role of miRNAs in systems-level pathogenesis during OP development. In this article, literature-reported OP miRNAs were manually collected and analyzed based on a systems biology paradigm. Functional enrichment studies were performed to decode the underlying mechanisms of miRNAs in OP etiology and therapeutics in three-dimensional space, i.e., integrated miRNA-gene-pathway analysis. In particular, interactions between miRNAs and three well-known OP pathways, i.e., estrogenendocrine, WNT/β-catenin signaling, and RANKL/RANK/OPG, were systematically investigated, and the effects of non-genetic factors on personalized OP prevention and therapy were discussed. This article is a comprehensive review of OP miRNAs, and bridges the gap between an understanding of OP pathogenesis and clinical translation.

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(−)-Epicatechin Gallate (ECG) Stimulates Osteoblast Differentiation via Runt-related Transcription Factor 2 (RUNX2) and Transcriptional Coactivator with PDZ-binding Motif (TAZ)-mediated Transcriptional Activation

Cited by 46 publications

References 44 publications

Sulfuretin induces osteoblast differentiation through activation of TGF-β signaling

Sulfuretin induces osteoblast differentiation through activation of TGF-β signaling

Watercress-based electrospun nanofibrous scaffolds enhance proliferation and stemness preservation of human adipose-derived stem cells

MicroRNA Alterations for Diagnosis, Prognosis, and Treatment of Osteoporosis: A Comprehensive Review and Computational Functional Survey

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