Epidemiologic study of human parvovirus B19 infection in East China

Abstract

Human parvovirus B19 (B19V) infection causes a number of diseases in humans, and, in some circumstances, can be life threatening. To understand the epidemiology of B19V infection in the greater metropolitan area of Hangzhou, East China, we performed surveys of IgM and IgG antibodies against B19V and quantification of B19V DNA, by using enzyme-linked immunosorbent assay and quantitative PCR, respectively, in plasma samples from diverse groups. These groups included anemia patients, Mycoplasma pneumonia- and Treponema pallidum-infected patients, HIV-positive individuals, and healthy blood donor volunteers. Our results demonstrated a low level of B19V IgG antibody presence, ranging from 21.9% to 41.8% in all the groups tested, suggesting a low prevalence of B19V infection in the area. Of note, we found that two healthy blood donors and one Mycoplasma pneumonia-infected patient had B19V IgM antibody among 1,290 plasma samples tested. The Mycoplasma pneumonia-infected patient had viremia with viral genome copies of 2.86 × 10(6) per ml of plasma. We detected a high rate of B19V DNA (7.1%) in HIV-positive injection drug users. Importantly, an amino acid mutation of P558S in the large non-structural protein NS1 was identified to be conserved among 14 B19V isolates from the HIV-positive group but not in the B19V isolate of the Mycoplasma pneumonia-infected patient, representing a hallmark of B19V isolates that circulate in HIV1-positive patients in the greater metropolitan area of Hangzhou, East China.

Detection of human parvovirus B19 in serum samples from children under 5 years of age with rash–fever illnesses in the Democratic Republic of the Congo

“…Indeed, according to data from the Ministry of Public Health, several outbreaks of measles-like rashes have occurred over the last 5 years in Katanga. As B19V IgM antibodies and DNA were found in sera from all provinces of DRC, it can be confirmed that B19V has spread countrywide, and this supports the hypothesis that B19V has a worldwide distribution ( Rezaei et al, 2016 , Zhang et al, 2016 , Toshev et al, 2014 , Tolfvenstam et al, 2000 , Schwarz et al, 1989 ).…”

Molecular prevalence of parvovirus B19 among HIV1-infected patients in Iran

“…In this study, using a real-time PCR assay, B19V nucleic acid was detected in 10% of persons infected with HIV-1. This prevalence was nearly similar to 7.1% rate in HIV1-infected drug users in Hangzhou (33), 13.1% rate in HIV1-positive cases in Iran (34), and almost twice in comparison with 4.5% rate in Sichuan, China (35).…”

Human Parvoviruses

“…B19V infection is common worldwide, showing regional epidemiological differences, with generally over one-half of the adult population having been exposed. The prevalence of B19V-specific antibodies in the population is age dependent, increasing from 2 to 20% in children Ͻ5 years old to 15 to 40% in children 5 to 18 years old and to 40 to 80% in the adult population, depending on both the assays used and the population (214)(215)(216)(217)(218)(219)(220)(221)(222)(223)(224)(225). Seroprevalence, however, is much lower in some isolated areas, such as the Rodriguez Islands and among some Brazilian tribes, with adult seroprevalences of only 2 to 10% (226,227).…”