Epidemiological analysis of Salmonella enterica from beef sampled in the slaughterhouse and retailers in Dakar (Senegal) using pulsed-field gel electrophoresis and antibiotic susceptibility testing

Stevens, A.J.; Kérouanton, Annaëlle; Marault, Muriel; Millemann, Yves; Brisabois, Anne; Cavin, Jean-François; Dufour, Barbara

doi:10.1016/j.ijfoodmicro.2008.01.007

Cited by 33 publications

(28 citation statements)

References 33 publications

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“…PFGE has been demonstrated to be sufficiently discriminatory for subtyping different Salmonella serovars, including S. Corvallis [6,32,33]. Our finding that the genetic patterns of S. Corvallis recovered from different areas in the poultry processing plant were indistinguishable suggests that this strain is well adapted to the conditions at this plant.…”

Section: Discussionmentioning

confidence: 66%

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Yamatogi

Oliveira

Camargo

et al. 2015

J Infect Dev Ctries

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Introduction: Salmonella is a major cause of foodborne disease, and poultry products are important contributors to the transmission of this zoonotic pathogen. Although considered to be rare in most countries, Salmonella Corvallis has been reported in specific geographic areas isolated from both human and non-human sources. The aim of this study was to report the occurrence, the antimicrobial resistance profiles including the extended-spectrum beta-lactamase (ESBL) production, and the clonal relatedness of S. Corvallis strains. Methodology: A total of 132 fragments of poultry carcasses from a slaughterhouse in São Paulo State, Brazil, were collected at different stages of the manufacturing process (post-bleeding, post-plucking, and post-chilling) and analyzed for the presence of Salmonella. Antimicrobial resistance was determined by disc diffusion method and Etest. Clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE). Results: Among the 272 Salmonella strains recovered, fourteen were S. Corvallis. Ten (71.4%) showed ESBL production and resistance to at least three antimicrobial agents. Nalidixic acid resistance and reduced ciprofloxacin susceptibility was verified in four (28.6%) strains. PFGE analyses showed that all the S. Corvallis strains belonged to the same pulsotype. Conclusion: This study identified genetically related S. Corvallis strains exhibiting ESBL production and reduced susceptibility to quinolone. The results suggest the need to improve the sanitary conditions in the slaughterhouse. Moreover, from a public health perspective, continuous surveillance on Salmonella is needed to control the dissemination of this important zoonotic pathogen and its resistance determinants.

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Section: Discussionmentioning

confidence: 66%

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Yamatogi

Oliveira

Camargo

et al. 2015

J Infect Dev Ctries

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“…Only XbaI was used in this study, as it is a more discriminative and cheaper reagent, and it has been shown to be a useful restriction enzyme for Salmonella spp [35].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Ammari

Laglaoui²,

En-nanei

et al. 2009

J Infect Dev Ctries

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Objectives: This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods. Methodology: Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimicrobial susceptibility testing, and PCR assay targeting the spvR and invA genes. Results: Six fingerprinting profiles were obtained with the ERIC-PCR method, four with PFGE profiling, five with antimicrobial resistance, three with phage typing, and only one with plasmid profiling. spvR gene was detected in six strains, which did not harbour plasmids of 90 kb. Conclusions: The conclusions of this study are drawn from a limited number of isolates. It would be desirable to investigate a greater and more diverse population of Salmonella isolates. S. Enteritidis was genotyped and showed four different patterns by PFGE and six by ERIC-PCR. Accordingly, high genetic similarity and limited genetic diversity were found for these strains from north of Morocco.

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“…However, relatively few serotypes have been isolated from cattle and bovine carcasses as well as beef meat. It has been shown that the presence of S. Typhimurium in cattle and the consequent cross contamination of beef carcasses are of particular concern since this serotype is one of the most common causes of human salmonellosis in many countries (3,5,9,20,21). Furthermore, many such strains have developed resistance to several antimicrobial agents (1).…”

Section: Introductionmentioning

confidence: 99%

“…To assess the routes of transmission and molecular relatedness of Salmonella isolates along the food chain, usually the pulsed field gel electrophoresis (PFGE) method is applied due to its discriminatory power and reproducibility (6,21). This approach allows establishing routes and sources of carcass contamination with these pathogenic bacteria at abattoirs and at the meat level.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and characterisation of Salmonella in slaughtered cattle and beef in Poland

Wieczorek

Osek

2013

Bulletin of the Veterinary Institute in Pulawy

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A total of 1229 samples, including 406 bovine hides and 406 corresponding carcasses at the slaughter level, as well as 417 beef meat from local supermarkets, were tested for the presence of Salmonella sp. Eighteen (1.5%) samples were positive for the target microorganism, and the highest prevalence (2.2%) was found in meat, followed by carcasses (1.2%) and hides (1.0%). Among the isolated strains, Salmonella enterica serotypes Enteritidis (six isolates) and Schleissheim (six strains), followed by Dublin (four contaminated samples) were the most predominant. The antimicrobial resistance analysis against nine antimicrobials with the MIC technique revealed that most isolates were sensitive to all antibacterial agents. However, one S. Typhimurium of carcass origin was multidrug resistant, and displayed the resistance to four antimicrobials, i.e. ampicillin, streptomycin, tetracycline, and sulphametoxazole. Furthermore, one S. Enteritidis (from carcasses), two S. Dublin (of beef origin), and one S. London (from meat) strains were resistant to sulphametoxazole. The restriction enzyme analysis with XbaI resulted in eight different PFGE types. The obtained results suggest that cattle may be an underestimated source of pathogenic Salmonella for consumers.

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Epidemiological analysis of Salmonella enterica from beef sampled in the slaughterhouse and retailers in Dakar (Senegal) using pulsed-field gel electrophoresis and antibiotic susceptibility testing

Cited by 33 publications

References 33 publications

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Prevalence and characterisation of Salmonella in slaughtered cattle and beef in Poland

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