Epidemiological investigation of community-acquired Staphylococcus aureus infection

Zhou, Zeng-Tong; Zhang, K.; Yu, Yong

doi:10.4238/2013.december.19.11

Cited by 12 publications

(13 citation statements)

References 25 publications

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“…However, it should be noted that a considerable proportion of ST88 S. aureus strains carried pvl gene [29], a pore-forming toxin gene that plays a crucial role in the pathogenicity of S. aureus [9]. Five of the six t377 strains in this study belonged to ST630-MSSA, ST630-MSSA-t377 strains presented as sporadic infections or relatively dominant strains in China [30,31], but the strong biofilm-forming capacity had not been realized in previous studies. Our results suggest that special attention should be paid to the biofilm infections caused by ST88 and ST630-MSSA-t377 strains, and larger epidemiological investigations are needed to verify their very strong biofilm-forming capacity and to explore the underlying mechanisms.…”

Section: Discussionmentioning

confidence: 58%

Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus

Jiang

Jia

et al. 2020

Ann Clin Microbiol Antimicrob

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Background: Staphylococcus aureus is a primary pathogen of orthopedic infections. By mediating antimicrobial resistance, S. aureus biofilm plays an important role in the recalcitrance of orthopedic infections, especially for the intractable osteomyelitis (OM). This study investigated the relationship between biofilm production and various genetic or phenotypic characteristics among orthopedic S. aureus strains. Methods: A total of 137 orthopedic S. aureus isolates were enrolled and divided into OM and non-OM groups. Biofilm production was evaluated using the crystal violet assay. Genetic and phenotypic characteristics including MRSA identification, MLST and spa typing, carriage of virulence genes, drug resistance, and patients' inflammatory responses indicators were characterized. The relationship between biofilm production and above-mentioned features was respectively analyzed among all isolates and compared between OM and non-OM isolates. Results: Biofilm production presented no significant difference between OM (including 9 MRSA isolates) and non-OM (including 21 MRSA isolates) strains. We found that ST88, t377 and ST630-MSSA-t377 strains produced very strong biofilms, while MLST types of ST15, ST25, ST398, ST5, ST59 and spa types of t002, t2325, t437 tended to produce weaker biofilms. Strains with the following profiles produced stronger biofilms: fib(+)-hlgv(+)-lukED(+)-sei(-)-sem(-)seo(-) for all isolates, sei(-)-sem(-)-seo(-) for OM isolates, and cna (+)-fib (+)-hlgv (+)-lukED (+)-seb(-)-sed(-) for non-OM isolates. In addition, not any single drug resistance was found to be related to biofilm production. We also observed that, among OM patients, strains with stronger biofilms caused weaker inflammatory responses. Conclusion: Some genetic or phenotypic characteristics of orthopedic strains were associated with biofilm production, and this association could be different among OM and non-OM strains. The results are of great significance for better understanding, evaluating and managing different kinds of biofilm-associated orthopedic infections, and provide potential targets for biofilm clearance.

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Section: Discussionmentioning

confidence: 58%

Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus

Jiang

Jia

et al. 2020

Ann Clin Microbiol Antimicrob

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“…Among the first six spa types of MSSA isolates, t189 was also identified as predominant in Malaysian and China [35], [36], t034, which always corresponds to ST398, was characterized as livestock-associated isolates and prevalent among isolates from skin or soft tissue infection in many countries including China [37]–[39], t078 was recently found to be associated with community acquired skin and soft tissue infection [40], t091 has recently been showed to be a major genotype of MSSA isolated from bacteriaemia in China [41], t309 and t377 were seldom noted for Chinese isolates previously. In general, MSSA possessed a high genetic diversity, which suggested that the epidemic MRSA isolates were more likely to be imported or clone transmitted instead of arisen from successful MSSA clones and is in accordance with previous studies [6], [42].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Staphylococcus aureus from Distinct Geographic Locations in China: An Increasing Prevalence of spa-t030 and SCCmec Type III

et al. 2014

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Staphylococcus aureus belongs to one of the most common bacteria causing healthcare and community associated infections in China, but their molecular characterization has not been well studied. From May 2011 to June 2012, a total of 322 non-duplicate S. aureus isolates were consecutively collected from seven tertiary care hospitals in seven cities with distinct geographical locations in China, including 171 methicillin sensitive S. aureus (MSSA) and 151 MRSA isolates. All isolates were characterized by spa typing. The presence of virulence genes was tested by PCR. MRSA were further characterized by SCCmec typing. Seventy four and 16 spa types were identified among 168 MSSA and 150 MRSA, respectively. One spa type t030 accounted for 80.1% of all MRSA isolates, which was higher than previously reported, while spa-t037 accounted for only 4.0% of all MRSA isolates. The first six spa types (t309, t189, t034, t377, t078 and t091) accounted for about one third of all MSSA isolates. 121 of 151 MRSA isolates (80.1%) were identified as SCCmec type III. pvl gene was found in 32 MSSA (18.7%) and 5 MRSA (3.3%) isolates, with ST22-MSSA-t309 as the most commonly identified strain. Compared with non-epidemic MRSA clones, epidemic MRSA clones (corresponding to ST239) exhibited a lower susceptibility to rifampin, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, a higher prevalence of sea gene and a lower prevalence of seb, sec, seg, sei and tst genes. The increasing prevalence of multidrug resistant spa-t030 MRSA represents a major public health problem in China.

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“…Thereafter, ST121 MSSA isolates were found to be distributed in 15 out of the 19 surveyed countries, namely Paraguay, New Caledonia, Togo, France, Czech Republic, Germany, Turkey, the United States, French West Indies, United Kingdom, Polynesia, Switzerland, Spain, Algeria and The Netherlands. 21 A small study performed in mainland China demonstrated that ~8.3% (1/12) of SSTIs 22 and 30.6% of asymptomatically colonized children in kindergartens 23 were associated with ST121 isolates. These findings suggest that most ST121 strains are MSSA.…”

Section: Discussionmentioning

confidence: 99%

“…Although previous studies reported on S. aureus biogeography and virulence, 4 to our knowledge, this is the first study to compare contemporaneous CA-MRSA of SSTIs from adults and children and the first such study carried out in mainland China.…”

Section: Introductionmentioning

confidence: 96%

Characterization of community-associated Staphylococcus aureus from skin and soft-tissue infections: a multicenter study in China

Liu

Yang

et al. 2016

Emerging Microbes & Infections

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We evaluated the epidemiological and molecular features of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and methicillin-sensitive S. aureus (MSSA) from children and adult patients with skin and soft-tissue infections (SSTIs) in China. Prospective community-acquired S. aureus SSTI surveillance was conducted in 23 hospitals over a 24-month period. Susceptibility to 16 antimicrobials was evaluated using the agar dilution method. StatApriori was used to determine statistically significant association trends. The genotypic characteristics of CA-MRSA isolates were tested by staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) typing, and multilocus sequence typing. The presence of Panton–Valentine leukocidin (pvl) genes was determined. Overall, 71.6% (1946/2716) of cases were community-associated S. aureus. CA-MRSA accounted for 2.6% (51). Out of 1895 methicillin-sensitive S. aureus strains, 97.3% were resistant to erythromycin, 96.6% to penicillin and 89.1% to clindamycin. No S. aureus strains were resistant to vancomycin. Thirteen sequence types (STs) and 17 spa types were detected among the CA-MRSA strains. The most prevalent sequence type was ST121 (19/51, 37.3%), followed by ST59 (13/51, 25.5%). In addition, t437 was predominant, accounting for 43.1% (22/51). Only five (9.8%) of the CA-MRSA strains harbored pvl genes. There were no significant differences in antibiotic sensitivity profiles between ST121 and non-ST121 MRSA isolates. However, ST121 strains tended to be more resistant to cefazolin, whereas non-ST121 strains were more resistant to chloramphenicol. In conclusion, CA-MRSA infections are rare among Chinese SSTI patients. MRSA strains in China have diverse genetic backgrounds, with ST121 being the predominant clone. Fusidic acid and mupirocin remain effective for topical treatment.

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Epidemiological investigation of community-acquired Staphylococcus aureus infection

Cited by 12 publications

References 25 publications

Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus

Investigation of biofilm production and its association with genetic and phenotypic characteristics of OM (osteomyelitis) and non-OM orthopedic Staphylococcus aureus

Characterization of Staphylococcus aureus from Distinct Geographic Locations in China: An Increasing Prevalence of spa-t030 and SCCmec Type III

Characterization of community-associated Staphylococcus aureus from skin and soft-tissue infections: a multicenter study in China

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