Epidemiological relationships of Campylobacter jejuni strains isolated from humans and chickens in South Korea

Jeong

et al. 2017

IJERPH

This study examined antibiotic susceptibility, genetic diversity, and characteristics of virulence genes in Campylobacter isolates from poultry. Chicken (n = 152) and duck (n = 154) samples were collected from 18 wet markets in Korea. Campylobacter spp. isolated from the carcasses were identified by PCR. The isolated colonies were analyzed for antibiotic susceptibility to chloramphenicol, amikacin, erythromycin, tetracycline, ciprofloxacin, nalidixic acid, and enrofloxacin. The isolates were also used to analyze genetic diversity using the DiversiLabTM system and were tested for the presence of cytolethal distending toxin (cdt) genes. Campylobacter spp. were isolated from 45 poultry samples out of 306 poultry samples (14.7%) and the average levels of Campylobacter contamination were 22.0 CFU/g and 366.1 CFU/g in chicken and duck samples, respectively. Moreover, more than 90% of the isolates showed resistance to nalidixic acid and ciprofloxacin. Genetic correlation analysis showed greater than 95% similarity between 84.4% of the isolates, and three cdt genes (cdtA, cdtB, and cdtC) were present in 71.1% of Campylobacter isolates. These results indicate that Campylobacter contamination should be decreased to prevent and treat Campylobacter foodborne illness.

Section: Resultsmentioning

confidence: 99%

Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry

Jeong

et al. 2017

IJERPH

“…Many of the more common STs found in Australia, such as ST825, ST827 and ST50, have been isolated from various sources globally. C. jejuni isolates were dominated by ST50 which has been found in poultry in Israel [54], Poland [55], Denmark [56] and South Korea [57], among other countries. To date, ST50 has over 3,500 submissions on the PubMLST database.…”

Section: Discussionmentioning

confidence: 97%

Molecular characterization of Campylobacter spp. recovered from beef, chicken, lamb and pork products at retail in Australia

et al. 2020

Australian rates of campylobacteriosis are among the highest in developed countries, yet only limited work has been done to characterize Campylobacter spp. in Australian retail products. We performed whole genome sequencing (WGS) on 331 C. coli and 285 C. jejuni from retail chicken meat, as well as beef, chicken, lamb and pork offal (organs). Campylobacter isolates were highly diverse, with 113 sequence types (STs) including 38 novel STs, identified from 616 isolates. Genomic analysis suggests very low levels (2.3-15.3%) of resistance to aminoglycoside, beta-lactam, fluoroquinolone, macrolide and tetracycline antibiotics. A majority (>90%) of isolates (52/56) possessing the fluoroquinolone resistanceassociated T86I mutation in the gyrA gene belonged to ST860, ST2083 or ST7323. The 44 pork offal isolates were highly diverse, representing 33 STs (11 novel STs) and harboured genes associated with resistance to aminoglycosides, lincosamides and macrolides not generally found in isolates from other sources. Prevalence of multidrug resistant genotypes was very low (<5%), but tenfold higher in C. coli than C. jejuni. This study highlights that Campylobacter spp. from retail products in Australia are highly genotypically diverse and important differences in antimicrobial resistance exist between Campylobacter species and animal sources.

Comp Rev Food Sci Food Safe

“…It can also detect the level of genetic diversity of species and predict interspecies transmission, as reported for experiments on pig and poultry farms (Ceuppens and others ), and to determine genetic relatedness between C. jejuni and C. coli isolates from retail meat sources (Noormohamed and Fakhr ) and Campylobacter isolates from chicken flocks on 2 farms (Zhang and others ). MLST has also been used in reports of outbreaks and for determining public health cases (Schleihauf and others ) and epidemiological relationships of C. jejuni strains from chickens and humans (Oh and others ). In all these studies, STs and clonal complexes were assigned using the C. jejuni or C. coli PubMLST database.…”

Section: Molecular Tools Used For Typing Campylobacter Spp In Foods mentioning

confidence: 99%

“…Thus, it is easier and faster to employ molecular biology techniques such as polymerase chain reaction (PCR) and sequencing for identification purposes. In addition, tools such as pulsed‐field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) are the currently the most commonly used for typing (Goering ; Miller and others ; Behringer and others ; Gharst and others ; Manfreda and others ; Pan and others ; Vidal and others ; Oh and others ; Schleihauf and others ; Vinueza‐Burgos and others ; Zhang and others ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin

Frasão

Marin

Conté-Júnior

2017

Abstract:The most frequently reported zoonosis and the main bacterial foodborne disease infection in humans is caused by Campylobacter spp., and C. jejuni and C. coli are the most common types. These bacteria can be found in the intestinal tracts of cattle, dogs, cats, sheep, poultry and pigs. The isolation of this microorganism is laborious because it requires specific media and a low oxygen concentration for growth. Additionally, differentiation between species through conventional bacteriology is difficult, as there are few different biochemical characteristics among the various species. Molecular microbiological techniques have become more important and are now broadly applied to help overcome difficulties in the identification, differentiation, and quantification of this pathogen. To date, there have been advances in the development and use of molecular techniques for the identification of microorganisms in foodstuffs. Tools such as pulsed-field gel electrophoresis and multilocus sequence typing are the most commonly used for typing. For the identification and confirmation of species, polymerase chain reaction (PCR) is crucial. Quantification by real-time PCR has wide applicability. To identify strains and antimicrobial resistance genes, sequencing technologies have been applied. This review builds on the discussion about the main and most widely used molecular methods for Campylobacter, as well as methods showing better potential for the classification, identification, and quantification of this important pathogen.