Encapsulation of
active agents, such as vitamins and antioxidants,
is one of the possibilities that allow their incorporation in beverages,
food, or in pharmaceutical products. Simultaneously, encapsulation
protects these active agents from oxidation, producing more stable
active compounds. Formation of nanodroplets by spontaneously formed
microemulsion (ME) offers, on one hand, a low-energy technology of
encapsulation and, on the other hand, because of a small size of the
droplets, it assures long-term stability even in harsher environments.
In this study, oil-in-water MEs allowed the low-energy encapsulation
of α-tocopherol (αToc) into an aqueous medium with the
aid of fully food-grade ingredients, using isoamyl acetate as the
dispersed oil phase, which was selected between three different types
of oils. Both cosurfactant-free and cosurfactant-holder ME systems
were formulated, in which Tween 20 and glycerol were employed as the
surfactant and the cosurfactant, respectively. The ME monophasic area
was determined through the construction of pseudoternary phase diagrams.
The encapsulated αToc within 10–20 nm nanocapsules showed
radical scavenging activity dependent on the encapsulated amount of
αToc, as it was demonstrated by electron paramagnetic resonance
spectroscopy. The radical scavenging activity slightly increased within
the time investigated, indicating a slow release of the active compound
from the nanodroplets, which is a promising result for their application,
especially in pharmaceuticals.