Epidemiology and clinical characteristics of respiratory syncytial virus infections among children and adults in Mexico

Gamiño-Arroyo, Ana Estela; Moreno-Espinosa, Sarbelio; Llamosas-Gallardo, Beatriz; Ortiz-Hernández, Ana A.; Guerrero, M. Lourdes; Galán-Herrera, Juan Francisco; Prado-Galbarro, Francisco Javier; Beigel, John H.; Ruíz-Palacios, Guillermo M.; Noyola, Daniel E.

doi:10.1111/irv.12414

Cited by 33 publications

(33 citation statements)

References 25 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…RSV was the most predominant respiratory virus detected in the patient's samples (35.4% of the patients), while HRV was the second prevalent virus (18.5% of the patients). Our observation is in agreement with others who showed that RSV is the most prevalent virus in patients with respiratory tract infections . In addition to single viral infections, our result demonstrated that mixed viral infections are a frequent phenomenon; it was observed in seven samples indicating that more than one virus can trigger respiratory tract infections and that may influence the severity and outcomes of the respiratory disease.…”

Section: Discussionsupporting

confidence: 93%

Metagenomic analysis of viral diversity in respiratory samples from patients with respiratory tract infections in Kuwait

Madi

Al‐Nakib

Mustafa

et al. 2017

Journal of Medical Virology

View full text Add to dashboard Cite

A metagenomic approach based on target independent next-generation sequencing has become a known method for the detection of both known and novel viruses in clinical samples. This study aimed to use the metagenomic sequencing approach to characterize the viral diversity in respiratory samples from patients with respiratory tract infections. We have investigated 86 respiratory samples received from various hospitals in Kuwait between 2015 and 2016 for the diagnosis of respiratory tract infections. A metagenomic approach using the next-generation sequencer to characterize viruses was used. According to the metagenomic analysis, an average of 145, 019 reads were identified, and 2% of these reads were of viral origin. Also, metagenomic analysis of the viral sequences revealed many known respiratory viruses, which were detected in 30.2% of the clinical samples. Also, sequences of non-respiratory viruses were detected in 14% of the clinical samples, while sequences of non-human viruses were detected in 55.8% of the clinical samples. The average genome coverage of the viruses was 12% with the highest genome coverage of 99.2% for respiratory syncytial virus, and the lowest was 1% for torque teno midi virus 2. Our results showed 47.7% agreement between multiplex Real-Time PCR and metagenomics sequencing in the detection of respiratory viruses in the clinical samples. Though there are some difficulties in using this method to clinical samples such as specimen quality, these observations are indicative of the promising utility of the metagenomic sequencing approach for the identification of respiratory viruses in patients with respiratory tract infections.

show abstract

Section: Discussionsupporting

confidence: 93%

Metagenomic analysis of viral diversity in respiratory samples from patients with respiratory tract infections in Kuwait

Madi

Al‐Nakib

Mustafa

et al. 2017

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“…Even with a cutoff of >40% genome coverage, we identi ed RSV in 79% (although most RSV infections in children are thought to be symptomatic) and CoVs in 47% of HC samples ( Supplementary Table S1). Similarly, this study revealed a high frequency (56%) of RSV-A and RSV-B co-infections in RSV-ARI samples, while previous studies have shown RSV-A and RSV-B coinfections ranging from 0.1% to 0.4% of RSV-ARI 43,44 . The frequency of RSV coinfections was greater in the RSV severe group (69.6%) compared to RSV mild group (37.5%) (Figure 2b).…”

Section: Discussionsupporting

confidence: 71%

Metatranscriptomics to characterize respiratory virome, microbiome, and host response directly from clinical samples

Rajagopala

Bakhoum

Pakala

et al. 2020

Preprint

View full text Add to dashboard Cite

We developed a metatranscriptomics method that can simultaneously capture the respiratory virome, microbiome, and host response directly from low-biomass clinical samples. Using nasal swab samples, we have demonstrated that this method captures the comprehensive RNA virome with sufficient sequencing depth required to assemble complete genomes. We find a surprisingly high-frequency of Respiratory Syncytial Virus (RSV) and Coronavirus (CoV) in healthy children, and a high frequency of RSV-A and RSV-B co-infections in children with symptomatic RSV. In addition, we have characterized commensal and pathogenic bacteria, and fungi at the species-level. Functional analysis of bacterial transcripts revealed H. influenzae was highly active in symptomatic RSV subjects. Host transcriptome shows upregulation of the innate immune system, antiviral response and inflammasome pathway, and down regulation of fatty-acids pathway. This method is broadly applicable to infer transcriptome landscape of an infected system, surveil respiratory infections, and to sequence RNA viruses directly from clinical samples.

show abstract

“…This analysis is based on data from ILI-002, a hospital-based prospective observational cohort study of ILI [17][18][19]. The present analysis includes all participants enrolled in the ILI-002 study in whom 1 or 2 viruses were detected.…”

Section: Study Populationmentioning

confidence: 99%

Comparison of rates of hospitalization between single and dual virus detection in a Mexican cohort of children and adults with influenza-like illness

Noyola

Hunsberger

Salgado

et al. 2019

Open Forum Infectious Diseases

Self Cite

View full text Add to dashboard Cite

Background. Molecular detection methods allow for the simultaneous detection of several infectious agents. This study assesses whether co-infection with 2 viruses as compared with 1 is associated with increased hospitalization in those with acute respiratory infections.Methods. We prospectively enrolled a cohort of pediatric and adult participants with influenza-like illness during 2010-2014 in Mexico. Clinical information and respiratory samples were collected at enrollment. Respiratory viruses were detected with multiplex polymerase chain reaction (PCR) and influenza-specific reverse transcription PCR assays. Participants were followed for 14 and 28 days after inclusion. Severity of disease, as measured by hospitalization with acute respiratory infections, was compared between single and dual viral infections.Results. Among 5662 participants in the study, either 1 (n = 3285) or 2 (n = 641) viruses were detected in 3926 participants. Rhinovirus (n = 1433), influenza (n = 888), and coronaviruses (n = 703) were the most frequently detected viruses (either alone or in co-infection). Bocavirus, respiratory syncytial virus (RSV), metapneumovirus, and rhinovirus cases were hospitalized more often than other viruses. Bocavirus+rhinovirus cases were hospitalized more often than those with rhinovirus alone (but not bocavirus alone). RSV cases were more likely to be hospitalized than cases with co-infections of RSV and parainfluenza virus or coronavirus. Metapneumovirus cases were hospitalized more often than those co-infected with metapneumovirus+coronavirus.Conclusions. In this study, detection of 2 viruses did not significantly increase hospitalizations compared with single virus infections. Larger studies will allow for distinguishing between sequential and simultaneous infection and for a better understanding of the role of each virus during the evolution of acute respiratory episodes.

show abstract

Epidemiology and clinical characteristics of respiratory syncytial virus infections among children and adults in Mexico

Cited by 33 publications

References 25 publications

Metagenomic analysis of viral diversity in respiratory samples from patients with respiratory tract infections in Kuwait

Metagenomic analysis of viral diversity in respiratory samples from patients with respiratory tract infections in Kuwait

Metatranscriptomics to characterize respiratory virome, microbiome, and host response directly from clinical samples

Comparison of rates of hospitalization between single and dual virus detection in a Mexican cohort of children and adults with influenza-like illness

Contact Info

Product

Resources

About