Epidemiology and diagnosis of feline panleukopenia virus in Egypt: Clinical and molecular diagnosis in cats

“…Approximately 50 mg of the samples were homogenized in 1 ml of TRIzol® Reagent in Eppendorf tubes. Afterwards, total RNA was dissolved and preserved in diethylpyrocarbonate (DEPC)-treated water up to use [22,23].…”

Section: Molecular Studymentioning

confidence: 99%

“…Afterwards, the tubes of the reaction were put in thermo-cycler machine for 60 min at 42°C, and then the reaction was terminated for 5 min at 99°C. The PCR products containing the cDNA were kept at -80°C up to use for DNA amplification [22,23].…”

Section: Molecular Studymentioning

confidence: 99%

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Rapid Approaches for Diagnosis of Canine Distemper Virus in Live and Dead Dogs in Egypt

Awad

2019

NIDOC-ASRT

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Paramyxovirridae, CDV is a negative single strand RNA. Enveloped, RNA of the virus encoding for 6 structural proteins: Fusion protein (F), Haemagglutinin protein (H), large polymerase (L), nucleoprotein capside (N), protein envelops matrix protein (M) and Phosphoprotein (P) [4,5]. Infection occurs via inhalation, CDV invade upper respiratory tract, lymphoid tissues (6), replicating inside macrophage and monocyte cells [7]. Incubation period varies from one to four weeks depend up on immune-status of dogs and strain virulence [6,8]. B ACkgRounD: Canine Distemper Virus infection (CDV) is a highly contagious disease of high morbidity and mortality rates in dogs. The causative virus is CDV which is a Morbillivirus, CDV is a pantropic virus characterized by multisystemic infection and high case fatality, with worldwide distribution, so rapid diagnosis and quarantine of the infected dogs with starting suitable treatment was required. The aim: this study aimed to achieve rapid diagnosis of canine distemper virus infection on ante mortem and post mortem aspects. Material and Methods: One healthy control disabled dog, 53 infected dogs with suggestive clinical signs for CDV infection were checked by (a) clinical examination; (b) Rapid immunochromatgrophy (IC) onconjunctival swabs (c) qRT-PCR on blood and tracheal exudates to confirm presence or absence of CDV, by expression analysis of CDV-F gene. Then all 53 examined dogs isolated and received supportive treatment but all died and disabled controlled one exposed to soft death. qRT-PCR were conducted on tissue samples from all 54 dogs for detection CDV-F gene expression values in different tissues.(d) Statistical analysis to study effect of sex and breed using Chi-square test, to evaluate sensitivity, specificity, accuracy, PPV, negative PV, respectively of used test and prevalence of CDV. Results: Clinical signs suggestive for CDV infection recorded in all 53 examined dogs, 24 of 54 dogs were positive for IC. Gene expression analysis test detected high values for CDV-F gene expression in tracheal exudates and blood samples of 36 live dogs, while the expression values were also high in tissue samples from different organs of 36 dead dogs. Statistical comparison of IC to qRT-PCR showed that values were 72%, 100%, 81.4%, 100% and 64.2 for sensitivity, specificity, accuracy, PPV, negative PV, respectively. No effect of sex , age, and breed on results using Chi-square test. Prevalence of CDV infection was 66% among population of this study. Conclusion: this study concluded that detection of clinical signs suggestive for CDV with application of IC and qRT-PCR together should be applied as rapid diagnosis on ante mortem level, while qRT-PCR could be used for rapid post mortem diagnosis of CDV infection.

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“…Feline panleukopenia, caused by feline parvovirus (FPV), is an acute and highly contagious disease [1]. It clinically manifests as vomiting, high fever, leukopenia and enteritis in infected animals [2]. FPV has been known to induce disease in cats since the 1920s [3].…”

mentioning

confidence: 99%

Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

Jiao

Zhang

Liu

et al. 2020

Viruses

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Feline panleukopenia, caused by feline parvovirus (FPV), is a highly infectious disease characterized by leucopenia and hemorrhagic gastroenteritis that severely affects the health of large wild Felidae. In this study, tiger FPV virus-like particles (VLPs) were developed using the baculovirus expression system. The VP2 gene from an infected Siberian tiger (Panthera tigris altaica) was used as the target gene. The key amino acids of this gene were the same as those of FPV, whereas the 101st amino acid was the same as that of canine parvovirus. Indirect immunofluorescence assay (IFA) results demonstrated that the VP2 protein was successfully expressed. SDS-PAGE and Western blotting (WB) results showed that the target protein band was present at approximately 65 kDa. Electron micrograph analyses indicated that the tiger FPV VLPs were successfully assembled and were morphologically similar to natural parvovirus particles. The hemagglutination (HA) titer of the tiger FPV VLPs was as high as 1:218. The necropsy and tissue sections at the cat injection site suggested that the tiger FPV VLPs vaccine was safe. Antibody production was induced in cats after subcutaneous immunization, with a >1:210 hemagglutination inhibition (HI) titer that persisted for at least 12 months. These results demonstrate that tiger FPV VLPs might provide a vaccine to prevent FPV-associated disease in the tiger.

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Epidemiology and diagnosis of feline panleukopenia virus in Egypt: Clinical and molecular diagnosis in cats

Cited by 37 publications

References 14 publications

Serological Diagnosis of Toxoplasmosis in Household Cats in Egypt

Serological Diagnosis of Toxoplasmosis in Household Cats in Egypt

Rapid Approaches for Diagnosis of Canine Distemper Virus in Live and Dead Dogs in Egypt

Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

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