Epidemiology and molecular characterisation of colistin-resistant Klebsiella pneumoniae isolates from immunocompromised patients in Tunisia

Tanfous, Farah Ben; Raddaoui, Anis; Chebbi, Yosra; Achour, Wafa

doi:10.1016/j.ijantimicag.2018.08.022

Cited by 20 publications

(21 citation statements)

References 17 publications

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“…However, in this study mcr-1 gene wasn't detected among E. coli (1) and K. pneumoniae (2) colistin resistant isolates. This result is in agreement with Tanfous et al, (2018); Emara et al, (2019), who reported that colistin mcr-1 gene was not detected among their phenotypically resistant isolates. This result could be attributed to the inability of PCR to predict colistin susceptibility as it doesn't exclude chromosomal colistin resistance, and there are new mcr genes responsible for colistin resistance that were not investigated in this study, according to WHO.…”

Section: Discussionsupporting

confidence: 92%

Colistin susceptibility among multidrug resistant Gram-negative bacilli isolated from Tertiary hospital in Egypt

Salam

Hager

2020

Novel Research in Microbiology Journal

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Colistin is considered as the last resort for treatment of bacterial infections caused by carbapenem-resistant Gram-negative bacilli (GNB). Colistin resistance can increase due to the spread of plasmid-borne mcr-1 gene. This study aimed to determine colistin susceptibility and to detect the presence of mcr-1 gene in the clinical isolates of GNB recovered from different clinical samples collected from

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Section: Discussionsupporting

confidence: 92%

Colistin susceptibility among multidrug resistant Gram-negative bacilli isolated from Tertiary hospital in Egypt

Salam

Hager

2020

Novel Research in Microbiology Journal

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“…These multi-drug resistant pathogens are considered to be superbugs which may pose a serious threat to human health, especially in cancer patients. Such multi-drug resistant Klebsiella pneumoniae was reported from bloodstream infection in Tunisia [5]. We confirmed a rarely observed Comamonas testosteroni in bloodstream infection which showed 100% resistance to commonly used antibiotics.…”

Section: Letter To the Editorsupporting

confidence: 81%

Bloodstream Infection in the Paediatric Cancer Patients: Bacteriological Profile and Drug Resistance Patterns in Shenzhen, China

Patil¹,

Lopes²,

Chen³

et al. 2019

Clin Microbiol

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Bloodstream Infections (BSI) remain a main cause of death in pediatric cancer patients. We present the first report on the microbiological profile of bacteremia and drug resistance patterns in Shenzhen, China. We examined the types of bloodstream infectious agents and their antimicrobial susceptibility pattern in the pediatric oncology patients, from Shenzhen Children's Hospital from January 2016 to October 2018. Acinetobacter baumannii (12.82%), Klebsiella pneumoniae (12.82%) and Staphylococcus epidermidis (34.61%) were highly prevalent in cancer patients with bloodstream infection. Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae were also predominant. 29% of isolates were resistant to more than two class of antibiotics so call them as multi-drug resistant isolates.

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“…The mobile colistin resistance-1 ( mcr ) gene is more commonly isolated from E. coli isolates than from K. pneumoniae , a finding that is supported by our study as well as by previous studies. 11 , 19 , 34 , 35 Additionally, a previous Egyptian study found that the eight colistin-resistant K. pneumoniae isolates were negative for mcr-1 gene. 17 Another study showed that two E. coli isolates and two K. pneumoniae isolates were colistin-resistant and none of them was positive for mcr-1 gene.…”

Section: Discussionmentioning

confidence: 93%

“…Moreover, the misuse of colistin in agriculture and the poultry industry may be the main cause of the high incidence of mcr-1 in bacteria isolated from animals and animal products. 34 …”

Section: Discussionmentioning

confidence: 99%

Detection of Plasmid-Mediated Mobile Colistin Resistance Gene (mcr-1) in Enterobacterales Isolates from a University Hospital

Anan¹,

El-Seidi²,

Mostafa³

et al. 2021

IDR

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Purpose Colistin represents one of the last treatment options for infections caused by multi-drug resistant (MDR) Enterobacterales . The emergence of a plasmid-mediated mobile colistin resistance-1 ( mcr-1 ) gene has raised serious concerns about its potential dissemination among bacteria. Methods In this study, we evaluated the chromogenic medium, CHROMID ® Colistin Resistance (COLR) agar, for the rapid detection of colistin-resistant Enterobacterales using broth microdilution (BMD) as a reference method. We also attempted to detect mcr-1 , −2 , −3 , −4 , and −5 genes, as well as the insertion sequence IS Apl1 via polymerase chain reaction (PCR), followed by sequencing of mcr gene(s). Results Among the 100 studied Enterobacterales isolates, 53% of them were colistin-resistant, with higher rate among Klebsiella pneumoniae (75%) as compared to Escherichia coli (44.4%). The COLR agar showed 83.2% sensitivity and 97.9% specificity for the detection of colistin resistance. Among colistin-resistant isolates, mcr-1 gene was only detected in four (7.5%) E. coli isolates. The IS Apl1 was not found among mcr-1 positive isolates. Sequencing of mcr-1 gene revealed nucleotide sequence homogeneity with the wild-type mcr-1 gene in BLAST. Conclusion The COLR agar is a promising phenotypic method for the detection of colistin-resistant Enterobacterales . Multiplex PCR followed by sequencing can be used for mcr genes’ detection and characterization.

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Epidemiology and molecular characterisation of colistin-resistant Klebsiella pneumoniae isolates from immunocompromised patients in Tunisia

Cited by 20 publications

References 17 publications

Colistin susceptibility among multidrug resistant Gram-negative bacilli isolated from Tertiary hospital in Egypt

Colistin susceptibility among multidrug resistant Gram-negative bacilli isolated from Tertiary hospital in Egypt

Bloodstream Infection in the Paediatric Cancer Patients: Bacteriological Profile and Drug Resistance Patterns in Shenzhen, China

Detection of Plasmid-Mediated Mobile Colistin Resistance Gene (mcr-1) in Enterobacterales Isolates from a University Hospital

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