Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran

Khanshour, Anas M.; Conant, Eleanore; Juras, Rytis; Cothran, E. Gus

doi:10.3906/sag-1202-80

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…18 Also, the study of Ghotaslou et al carried out on 51 patients with lower respiratory tract infection in Azerbaijan revealed that about 6.15% of them had M. pneumoniae. 17 Similar frequencies of this pathogen have been reported in other studies from other parts of the world, like those of Elkholy et al and Meijer et al involving 111 Egyptian children and 557 Dutch patients with the positivity rates of only 4.5% 19 and 1.3%, 20 respectively. However, a relatively high frequency of the infection (>19%) has been reported in our neighboring countries, such as Turkey.…”

Section: Discussionsupporting

confidence: 82%

“…16 Even though we recorded a high frequency (26.4%) of M. pneumoniae in our study, low frequencies (1-6.15%) have been reported in several studies in Iran. 17,18 The research conducted by Oskooee et al involving 102 CAP patients displayed the positivity rate of only 1% for M. pneumoniae. 18 Also, the study of Ghotaslou et al carried out on 51 patients with lower respiratory tract infection in Azerbaijan revealed that about 6.15% of them had M. pneumoniae.…”

Section: Discussionmentioning

confidence: 98%

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High frequency of Mycoplasma pneumoniae among patients with atypical pneumonia in Tehran, Iran

Arfaatabar¹,

Aminharati²,

Azimi³

et al. 2018

Germs

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Introduction Mycoplasma pneumoniae is a major cause of atypical community-acquired pneumonia) CAP) with a prevalence range of 15-20% and up to 40% in adults and children, respectively. In Iran, the recorded frequency ranges between 1-6.15%. We aimed to investigate the frequency of M. pneumoniae among patients with atypical pneumonia acquired from the community. Methods Over a period of 5 months between January and June 2017, 520 patients with suspected CAP, who had been to the hospital outpatient clinics of Tehran University, were enrolled in this study. Throat swab specimens were obtained from 110 outpatients who presented with symptoms of atypical pneumonia. M. pneumoniae was identified via culture and biochemical tests, such as fermentation of glucose and arginine, hemolysis, and hemadsorption. For confirmation, PCR was performed to amplify the gene fragment coding for p1 adhesin. Results The major and minor clinical signs of the patients were dyspnea (67.3%) and nausea (15.5%), respectively. Out of 110 specimens, 25 (22.7%) and 29 (26.4%) isolates were identified to be M. pneumoniae via culture and molecular assay, respectively. Comparing the results of the two methods, the PCR showed better sensitivity and rapidity for the detection of M. pneumoniae. There was a high congruence between culture and the PCR assay; kappa level was 'almost perfect' (κ=0.90). Conclusion This is the first report of high frequency of M. pneumoniae in our region. This finding can serve as baseline information for further investigation and confirmation of the potential epidemics of M. pneumoniae pneumonia in our community.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 98%

High frequency of Mycoplasma pneumoniae among patients with atypical pneumonia in Tehran, Iran

Arfaatabar¹,

Aminharati²,

Azimi³

et al. 2018

Germs

View full text Add to dashboard Cite

show abstract

“…The frequency of these microorganisms using culture in other regions including 10.33% in Japan (20), 2.01% in Iran (21), and 10.8% in Serbia (22) was different in comparison with that of the current study. There are some controversies about the gold standard method to diagnose M. pneumoniae.…”

Section: Discussionmentioning

confidence: 40%

Detection of Mycoplasma pneumoniae Among Children with Pneumonia Using Bacterial Culture, Polymerase Chain Reaction, and the Enzyme-linked Immunosorbent Assay Techniques in Ahvaz, Iran

Amin

Abadi

Navidifar

et al. 2018

Jundishapur J Microbiol

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Background: Mycoplasma pneumoniae is one of the most common causes of atypical pneumonia, which is almost asymptomatic and self-limited. Objectives: The current study aimed at investigating the prevalence of M. pneumoniae among children with pneumonia in Ahvaz, Iran, using bacterial culture growth, polymerase chain reaction (PCR), and serology tests. Methods: A total of 136 throat swab and serum specimens were collected from patients with pneumonia. The specimens were cultured on pleuropneumonia-like organisms (PPLO) agar. Molecular identification of the throat swab specimens was performed using the amplification of P1 gene. Determination of M. pneumoniae-specific antibodies (IgG and IgM) in the sera was carried out by the enzyme-linked immunosorbent assay (ELISA) technique. Results: In the current study, the acute infection was detected in 16 cases. Moreover, 3 out of 136 cases had positive results in their bacterial culture. Mycoplasma pneumoniae DNA was detected in 11 of the 136 cases. An acceptable titer of IgM was observed in 12 cases. On the other hand, 4-fold or greater titer of IgG was detected in 14 cases. Conclusions:The results of the current study suggested that the combination of PCR and the serology results were effective to detect M. pneumoniae. Moreover, the combination of PCR and IgM results can detect all cases of acute infection with M. pneumoniae in children.

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“…Overall, 53% of patients had elevated IgG level and only 5.3% had IgM positive test results, which indicated active M. pneumoniae infection (19). In a study in Greek, 225 children, who were hospitalized for respiratory tract infections were assessed regarding the presence of IgG and IgM antibodies by ELISA (20).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Mycoplasma pneumoniae Specific IgM and IgG Antibodies in Asymptomatic Preschool Aged Children of Tehran, Iran, 2010

Dashti

Hashemi

Halaji

et al. 2017

Arch Pediatr Infect Dis

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Epidemiology, clinical features, and laboratory detection of Mycoplasma pneumoniae infection in East Azerbaijan, Iran

Cited by 8 publications

References 52 publications

High frequency of Mycoplasma pneumoniae among patients with atypical pneumonia in Tehran, Iran

High frequency of Mycoplasma pneumoniae among patients with atypical pneumonia in Tehran, Iran

Detection of Mycoplasma pneumoniae Among Children with Pneumonia Using Bacterial Culture, Polymerase Chain Reaction, and the Enzyme-linked Immunosorbent Assay Techniques in Ahvaz, Iran

Seroprevalence of Mycoplasma pneumoniae Specific IgM and IgG Antibodies in Asymptomatic Preschool Aged Children of Tehran, Iran, 2010

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