Epidemiology of and prenatal molecular distinction between invasive and colonizing group B streptococci in The Netherlands and Taiwan

Elzakker, Epm Van; Yahiaoui, Rachid; Visser, Caroline E.; Oostvogel, Paul M.; Muller, Anouk E.; Ho, Yueh Ren; Wu, Jiunn Jong; Belkum, Alex van

doi:10.1007/s10096-009-0726-4

Cited by 7 publications

(6 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings corroborate the finding of other studies from industrialized country settings [31], [34], [36], [37]. Our study, however, clarified that the difference in serotype distribution between invasive and colonizing isolates is related to an increased invasive potential of certain GBS serotypes (i.e.…”

Section: Discussionsupporting

confidence: 92%

“…However, the invasive potential of serotype Ia is variable across different sites. In South Africa, Taiwan and The Netherlands [37], serotype Ia was less invasive than type III, although more invasive in Portugal [34], Israel [36] and Sweden [31]. The reasons for variation in invasive potential of serotype Ia reported at different sites are unclear, however data from Portugal suggests that the high invasive index of this serotype can be attributed to a dominant clone (ST-23 and ST-24), suggesting that the underlying genotype can influence the invasive potential.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Serotype Distribution and Invasive Potential of Group B Streptococcus Isolates Causing Disease in Infants and Colonizing Maternal-Newborn Dyads

et al. 2011

View full text Add to dashboard Cite

BackgroundSerotype-specific polysaccharide based group B streptococcus (GBS) vaccines are being developed. An understanding of the serotype epidemiology associated with maternal colonization and invasive disease in infants is necessary to determine the potential coverage of serotype-specific GBS vaccines.MethodsColonizing GBS isolates were identified by vaginal swabbing of mothers during active labor and from skin of their newborns post-delivery. Invasive GBS isolates from infants were identified through laboratory-based surveillance. GBS serotyping was done by latex agglutination. Serologically non-typeable isolates were typed by a serotype-specific PCR method. The invasive potential of GBS serotypes associated with sepsis within seven days of birth was evaluated in association to maternal colonizing serotypes.ResultsGBS was identified in 289 (52.4%) newborns born to 551 women with GBS-vaginal colonization and from 113 (5.6%) newborns born to 2,010 mothers in whom GBS was not cultured from vaginal swabs. The serotype distribution among vaginal-colonizing isolates was as follows: III (37.3%), Ia (30.1%), and II (11.3%), V (10.2%), Ib (6.7%) and IV (3.7%). There were no significant differences in serotype distribution between vaginal and newborn colonizing isolates (P = 0.77). Serotype distribution of invasive GBS isolates were significantly different to that of colonizing isolates (P<0.0001). Serotype III was the most common invasive serotype in newborns less than 7 days (57.7%) and in infants 7 to 90 days of age (84.3%; P<0.001). Relative to serotype III, other serotypes showed reduced invasive potential: Ia (0.49; 95%CI 0.31–0.77), II (0.30; 95%CI 0.13–0.67) and V (0.38; 95%CI 0.17–0.83).ConclusionIn South Africa, an anti-GBS vaccine including serotypes Ia, Ib and III has the potential of preventing 74.1%, 85.4% and 98.2% of GBS associated with maternal vaginal-colonization, invasive disease in neonates less than 7 days and invasive disease in infants between 7–90 days of age, respectively.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Serotype Distribution and Invasive Potential of Group B Streptococcus Isolates Causing Disease in Infants and Colonizing Maternal-Newborn Dyads

et al. 2011

View full text Add to dashboard Cite

show abstract

“…All isolates were previously identified as GBS using both biochemical and molecular methods [14]. Strains were stored at −80°C.…”

Section: Methodsmentioning

confidence: 99%

“…A subset of 33 colonizing and invasive GBS isolates representing all seven of the important subtypes was obtained from a reference collection (Table 1 ) [ 14 ]. All isolates were previously identified as GBS using both biochemical and molecular methods [ 14 ]. Strains were stored at −80°C.…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization

Witt

Oostvogel

Yahiaoui

et al. 2011

Eur J Clin Microbiol Infect Dis

Self Cite

View full text Add to dashboard Cite

A broth for the screening of group B streptococcal (GBS) carriage during pregnancy is about to be introduced. Simulating conditions in everyday practice, we have compared the sensitivity of this Granada tube broth (GT) with that of classical Amies transport medium (AT) in vitro. A total of 1,485 GT and 1,485 AT were tested with 33 well-characterized GBS strains in three different concentrations, five different incubation times, and three different temperatures. After initial incubation at room temperature (RT) or 4°C, GT were placed at 37°C. GT were scored for the presence of orange pigment. GT and AT were subcultured on blood agar (BA). Pigment was observed in 98% of GT incubated at 37°C. GBS could be cultured in 91%, 73%, and 55% of GT incubated at 37°C, RT, or 4°C, respectively. For AT, these percentages were only 20% at 37°C, 52% at RT, and 59% at 4°C. When GT initially incubated at RT or 4°C were subsequently incubated at 37°C, the sensitivity improved significantly. We conclude that GT is a more sensitive GBS transport and culture medium than the conventional method, especially for low inocula and prolonged transport/incubation times. GT does not exclude the presence of GBS, and should always be incubated at 37°C and subcultured on solid agar for optimal sensitivity.

show abstract

“…These virulence factors are coded in bacterial virulence genes, and these genes vary among GBS subtypes. Epidemiological research has consistently shown that specific GBS bacterial genotypes, such as CC17, are over-represented in invasive disease compared with colonization, strongly suggesting that invasive GBS strains have unique bacterial virulence genes [ 20 , 35 , 36 , 37 , 38 , 39 , 40 ]. The specificity of IAP strategies could possibly be improved not only by screening for GBS carriage, but also by incorporating multiple genetic markers into a single PCR test, making it possible to determine both the presence and the invasive potential of GBS bacteria [ 37 ].…”

Section: Maternal Perspectivementioning

confidence: 99%

Group B Streptococcus Early-Onset Disease: New Preventive and Diagnostic Tools to Decrease the Burden of Antibiotic Use

et al. 2023

View full text Add to dashboard Cite

The difficulty in recognizing early-onset neonatal sepsis (EONS) in a timely manner due to non-specific symptoms and the limitations of diagnostic tests, combined with the risk of serious consequences if EONS is not treated in a timely manner, has resulted in a low threshold for starting empirical antibiotic treatment. New guideline strategies, such as the neonatal sepsis calculator, have been proven to reduce the antibiotic burden related to EONS, but lack sensitivity for detecting EONS. In this review, the potential of novel, targeted preventive and diagnostic methods for EONS is discussed from three different perspectives: maternal, umbilical cord and newborn perspectives. Promising strategies from the maternal perspective include Group B Streptococcus (GBS) prevention, exploring the virulence factors of GBS, maternal immunization and antepartum biomarkers. The diagnostic methods obtained from the umbilical cord are preliminary but promising. Finally, promising fields from the newborn perspective include biomarkers, new microbiological techniques and clinical prediction and monitoring strategies. Consensus on the definition of EONS and the standardization of research on novel diagnostic biomarkers are crucial for future implementation and to reduce current antibiotic overexposure in newborns.

show abstract

Epidemiology of and prenatal molecular distinction between invasive and colonizing group B streptococci in The Netherlands and Taiwan

Cited by 7 publications

References 33 publications

Serotype Distribution and Invasive Potential of Group B Streptococcus Isolates Causing Disease in Infants and Colonizing Maternal-Newborn Dyads

Serotype Distribution and Invasive Potential of Group B Streptococcus Isolates Causing Disease in Infants and Colonizing Maternal-Newborn Dyads

In vitro evaluation of the performance of Granada selective enrichment broth for the detection of group B streptococcal colonization

Group B Streptococcus Early-Onset Disease: New Preventive and Diagnostic Tools to Decrease the Burden of Antibiotic Use

Contact Info

Product

Resources

About