Epidemiology of Equine Rotavirus Infection among Foals in the Breeding Region.

Imagawa, Hiroshi; Sekiguchi, Kiichi; Anzai, Tohru; Fukunaga, Yoshio; Kanemaru, Takumi; Ohishi, Hideo; Higuchi, Tohru; Kamada, Masanobu

doi:10.1292/jvms.53.1079

Cited by 36 publications

(30 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…72(9): 1247-1250, 2010 Equine rotavirus is a non-enveloped virus belonging to the genus Rotavirus in the family Reoviridae. Equine rotavirus infection is a major cause of diarrhea in foals up to 3 months of age [3,10]. Equine rotavirus has two outer capsid proteins, VP7 and VP4, which are independent neutralization antigens.…”

mentioning

confidence: 99%

Evaluation of Rapid Antigen Detection Kits for Diagnosis of Equine Rotavirus Infection

Nemoto

Hata

Higuchi

et al. 2010

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. We evaluated antigen detection kits for human rotavirus with regard to their usefulness for diagnosing equine rotavirus infection. Limiting dilution analyses showed that of the seven kits investigated the Dipstick 'Eiken' Rota (Dipstick) had the highest sensitivity to two serotypes of equine rotavirus. The Dipstick did not cross-react with several equine intestinal pathogens. An investigation using 249 fecal samples indicated that the sensitivity of the Dipstick was 81.9% and 47.3%, and its specificity was 98.2% and 99.0%, and its concordance rate was 92.8% and 68.3%, compared with values obtained using reverse transcription polymerase chain reaction and reverse transcription loop-mediated isothermal amplification, respectively. Although a negative result does not preclude the possibility of equine rotavirus infection, the Dipstick would be useful as routine test for diagnosing equine rotavirus infection in daily clinical practice because of its ease of handling.KEY WORDS: diagnosis, equine rotavirus, rapid antigen detection.J. Vet. Med. Sci. 72(9): 1247-1250, 2010 Equine rotavirus is a non-enveloped virus belonging to the genus Rotavirus in the family Reoviridae. Equine rotavirus infection is a major cause of diarrhea in foals up to 3 months of age [3,10]. Equine rotavirus has two outer capsid proteins, VP7 and VP4, which are independent neutralization antigens. VP7 and VP4 are classified as G (glycoprotein) serotypes by virus neutralization tests and P (protease-sensitive) genotypes by genetic analyses [14], respectively. Only G3 P[12] and G14 P[12] strains are currently considered to be circulating in the horse populations of Japan, Australia, Germany, Ireland and the United Kingdom [2,4,7,13,16]. Equine rotavirus infection has been diagnosed by electron microscopy, virus isolation, serological tests and molecular diagnostic methods [1,5]. These methods are not routinely available in diagnostic laboratories because they require sophisticated techniques and expensive equipment. It is difficult for clinical veterinarians to conduct these diagnostic methods as part of their daily clinical practice. Therefore, we focused our attention on rapid antigen detection (RAD) kits for use in routine tests in daily clinical practice. RAD kits do not require additional reagents, equipment or expertise, and results can be obtained within 15 min. RAD kits are designed to detect human group A rotaviruses based on the principle of latex agglutination assay (LA) or immunochromatographic assay. Because equine rotavirus is classified as a group A rotavirus, it is anticipated that RAD kits would also work for the rapid diagnosis of equine rotavirus infection. In fact, one LA kit (Virogen Rotatest, Wampole Laboratories, N.J., U.S.A.), which is not commercially available in Japan, has been reported to be useful for diagnosing equine rotavirus infection [6]. Although several RAD kits are available in Japan, their performance as regards the diagnosis of equine rotavirus infection has not been examined. In this study, we have ...

show abstract

mentioning

confidence: 99%

Evaluation of Rapid Antigen Detection Kits for Diagnosis of Equine Rotavirus Infection

Nemoto

Hata

Higuchi

et al. 2010

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Depression and colic are often observed in serious field cases. The disease occurs in suckling foals, especially those less than 4 months old [6]. Usually fluids and antibiotics are given to foals with severe diarrhea to prevent severe dehydration, electrolyte imbalances and secondary bacterial infection.…”

mentioning

confidence: 99%

“…The disease is highly contagious, but the mortality rate is extremely low. The disease occures severely in newborn foals in the Hidaka region of Hokkaido, a major thoroughbred breeding district of Japan [6]. The serotypes and genotypes of rotaviruses are classified by differences in the antigenicity of two kinds of viral outer capsid protein (VP4 and VP7) or the VP4 coding gene.…”

mentioning

confidence: 99%

“…It has been suggested that the G3P [12] rotavirus was predominant in surveys conducted on sera collected from yearlings in the same region in 1999, but the G14P [ 1 2 ] r o t a v i r u s h a s a l s o s p r e a d [ 8 ] . I n a n epidemiological study of foal diarrhea conducted by the authors and others in the breeding region for 7 years, it was found that approximately 30% of foal diarrhea is caused by rotavirus infection [6]. Therefore, many breeders and veterinarians have voiced the need for the development of an effective equine rotavirus vaccine.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Field Study of Inactivated Equine Rotavirus Vaccine

Imagawa

Kato

Tsunemitsu

et al. 2005

JES

Self Cite

View full text Add to dashboard Cite

show abstract

“…The first detection of equine RV has been reported in the year 1975 from England (Flewett et al, 1975). Since from this, equine RVs have been known to be the major causes of diarrhea in foals (Imagawa et al, 1991;Collins et al, 2008;Frederick et al, 2009). Based on the serological reports equine RVAs have been shown to be ubiquitous in the equine populations (Pearson et al, 1982).…”

Section: Rotavirusesmentioning

confidence: 99%

Evolving views on enteric viral infections of equines: an appraisal of key pathogens

Sircar¹,

Saurabh²,

Kattoor³

et al. 2016

JEBAS

View full text Add to dashboard Cite

Equines, the earliest known mammalian species, have been found highly susceptible to several enteric pathogens including viruses, fungi, parasites and bacteria. This review conserves with the key viral pathogens that affects foals and horses such as rotavirus, adenovirus, coronavirus, parvovirus, picobirnavirus etc. With the advent of next generation sequencing approaches the list of new enteric viruses has expanded. Viruses like Cyclovirus, Kirkovirus and Anellovirus are the new members identified in equines recently. Close proximity of horses to human settlements and/or other domestic animals pretense the threat of infectious diseases spread to humans/animals and vice-versa. Therefore, horse diseases are not only of veterinary importance but also are among important factors for public health. Herein, we intend to appraise current status of key enteric viruses that cause diarrheic disorders in foals and horses.

show abstract

Epidemiology of Equine Rotavirus Infection among Foals in the Breeding Region.

Cited by 36 publications

References 0 publications

Evaluation of Rapid Antigen Detection Kits for Diagnosis of Equine Rotavirus Infection

Evaluation of Rapid Antigen Detection Kits for Diagnosis of Equine Rotavirus Infection

Field Study of Inactivated Equine Rotavirus Vaccine

Evolving views on enteric viral infections of equines: an appraisal of key pathogens

Contact Info

Product

Resources

About