Epidemiology, zoonotic aspects, vaccination and control/eradication of brucellosis in India

Renukaradhya, G.J.; Isloor, S.; Rajasekhar, M.

doi:10.1016/s0378-1135(02)00253-5

Cited by 159 publications

(126 citation statements)

References 3 publications

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“…Long-term serological studies at national level indicated, 5% cattle infected with brucellosis [28].…”

Section: /5mentioning

confidence: 99%

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Kushwaha¹

2014

JMEN

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Brucellosis is a contagious systemic bacterial disease of livestock. Antibiotics commonly being used have limited efficacy against the disease. In the present study, therapy with antibiotics on Brucella infected cattle was performed and subsequently haemato-biochemical parameters, detection of anti-Brucella antibodies in both serum and milk by different diagnostics and shedding of Brucella in milk were assessed. Treatment with antibiotics; streptomycin and long acting tetracycline alone and in combination were given for four weeks. Hb, TEC, TLC, neutrophils, eosinophils, lymphocytes, monocytes count, AST, ALT and SD values were lower whereas, PCV, serum glucose, total protein, albumin and creatinine were higher in Brucella infected cattle. Treatment with combination therapy showed promising results in these animals. Haemato-biochemical parameters were become near to normal in most of animals that received combination therapy. However, not all animals were returned to normal after treatment but a significant numbers in combination therapy were reduced anti-Brucella antibody titre, both in serum and milk. The level of shedding of Brucella in milk had also gone undetectable. KeywordsBrucellosis; Cattle; Haemato-biochemical parameters; Oxytetracycline; Streptomycin Reaction (PCR) based assays have been developed for the rapid identification of Brucella. It is advantageous to detect DNA of pathogen that rendered biologically safe and hence, reduced the risk of infection to laboratory workers. In addition, PCR is more sensitive than traditional culture method [8].There is limited evidence on treatment aspect of Brucella infected cattle as well as their haemato-biochemical parameters. Brucella is inaccessible to antibiotics as they are facultative intracellular pathogens. Many antimicrobials are active against Brucella species; however, clinical efficacy does not always correlate with in vitro susceptibility [9]. Treatments for brucellosis have been tried previously but none was found successful [10,11]. Streptomycin acts synergistically with penicillin or tetracycline to inhibit the growth of B. abortus within bovine cells cultured in vitro [12]. Considering the above facts, the present work was intended to determine the efficacy of antibiotic therapy and alteration in the haemato-biochemical parameters if any, in Brucella infected cattle after treatment. Materials and MethodsA total of 27 cattle which showed antibodies both in serum and milk as well as Brucella DNA in milk were selected for the study. ELISA, RBPT and STAT were employed on serum whereas, MRT and ELISA on milk to detect the anti-Brucella antibodies. Milk samples were processed for detection of pathogen itself in form of its DNA (16S rRNA) by PCR [13]. Reference antigens and serum for RBPT, STAT and, for MRT, Abortus Bang Ring Antigen (ABR Antigen) were procured from the Indian Veterinary Research Institute, Izatnagar (India). AniGen B. Brucella Ab ELISA kit (Cat. No. EB43-01) (i-ELISA and Milk-ELISA) was procured

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“…Long-term serological studies at national level indicated, 5% cattle infected with brucellosis [28].…”

Section: /5mentioning

confidence: 99%

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Kushwaha¹

2014

JMEN

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“…The rate of infection varies greatly from one country to another and between regions within the country, with highest prevalence in dairy cattle. In India, brucellosis was first reported in 1942 and is now endemic throughout the country (Renukaradhya et al, 2002). In general, risk factors such as unrestricted trade and movements of animals, free grazing and movement with frequent mixing of flocks of sheep and goats also attribute to the high prevalence and wide distribution of brucellosis.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Detection of Brucella Antibodies in Sheep with Special Aspect of Clinicalstatus and Breed

Patel¹,

Chauhan²,

Patel³

et al. 2017

Adv. Anim. Vet. Sci.

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| Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. To ascertain the serological status of the disease, a total of 1373 samples were screened for the presence of Brucella antibodies by RBPT and i-ELISA. Overall seroprevalence recorded in sheep was 6.84% (94 samples out of 1373) and 4.80% (66 out of 1373) by RBPT and i-ELISA respectively. Clinical status wise seroprevalence was recorded in heifer 4.65% and 6.97%, clinically healthy animals 2.62% and 1.79%,animals with the history of abortion 16.58% and 13.98%, hygroma 27.27% and 18.18%,pregnant 4.59% and 2.29%, non-pregnant 6.80% and 4.18%, status unknown 10.78% and 6.86%, still birth 17.64% and 5.88%, retention of placenta 20.00% and 20.00%,orchitis16.12% and 9.67% respectively by RBPT and i-ELISA, respectively. Breedwise highest seroprevalence was recorded in Patanwadi 7.82% and 5.99% followed by Marwadi 6.35% and 4.16%,Magra 3.84% and 1.28%, Avikalin 4.76% and 0.00%and Chokhla 1.78% and 0.00%by RBPT and i-ELISA, respectively. Present study revealed that the Patanwadi breed of the sheep is relatively more susceptible and high rate of seroprevalence was observed from the animals having history of abortion, retention of placenta and hygroma.

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“…In addition, animals with polyarthritis have been observed in endemic flocks (Radostits, Gay, Hinchcliff, & Constable, 2006). Seropravelance studies from the backbone of epidemiology investigation and are used to identify herd infected with Brucella (Renukaradhya et al, 2002 (Pal, 2007). RBPT test has proven useful as a herd screening test and is highly sensitive (MacMillan, 1990).…”

Section: Introductionmentioning

confidence: 99%

Comparative Sensitivity and Specificity of Various Serological Tests for Detection of Brucellosis in Small Ruminants

Padher¹,

Nayak²,

Brahmbhatt³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Epidemiology, zoonotic aspects, vaccination and control/eradication of brucellosis in India

Cited by 159 publications

References 3 publications

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Detection of Brucella Antibodies in Sheep with Special Aspect of Clinicalstatus and Breed

Comparative Sensitivity and Specificity of Various Serological Tests for Detection of Brucellosis in Small Ruminants

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