2017
DOI: 10.1002/stem.2731
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Epidermal Growth Factor and Granulocyte Colony Stimulating Factor Signaling Are Synergistic for Hematopoietic Regeneration

Abstract: Hematopoietic regeneration following chemotherapy may be distinct from regeneration following radiation. While we have shown that epidermal growth factor (EGF) accelerates regeneration following radiation, its role following chemotherapy is currently unknown. We sought to identify EGF as a hematopoietic growth factor for chemotherapy-induced myelosuppression. Following 5-fluorouracil (5-FU), EGF accelerated hematopoietic stem cell regeneration and prolonged survival compared with saline-treated mice. To mitiga… Show more

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Cited by 11 publications
(8 citation statements)
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References 46 publications
(64 reference statements)
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“…Little is known about the effect of leptin or EGF in erythropoiesis and even less in prematurity, but it might be a mechanism to compensate the reduced number of erythrocytes. However, the effect of EGF raising the leukocyte count could be explained by its role in hematopoiesis, increasing cellular proliferation and decreasing the apoptosis of hematopoietic stem cells in mice [36,37].…”
Section: Discussionmentioning
confidence: 99%
“…Little is known about the effect of leptin or EGF in erythropoiesis and even less in prematurity, but it might be a mechanism to compensate the reduced number of erythrocytes. However, the effect of EGF raising the leukocyte count could be explained by its role in hematopoiesis, increasing cellular proliferation and decreasing the apoptosis of hematopoietic stem cells in mice [36,37].…”
Section: Discussionmentioning
confidence: 99%
“…At day 12 postinjection, colonies were counted independently by two investigators. LTC-IC limiting dilution analyses were performed as described previously (Piryani et al., 2018). For competitive transplantation assays, donor mice were exposed to 500 cGy TBI and then sacrificed at 7 days postirradiation.…”
Section: Methodsmentioning
confidence: 99%
“…Cell doses of 10 6 WBM donor cells and 2 × 10 5 nonirradiated host marrow were transplanted by single tail vein injection into 900-cGy irradiated recipient mice. Donor engraftment was measured in the BM at 12 weeks posttransplantation using flow cytometric analysis using published methods (Piryani et al., 2018).…”
Section: Methodsmentioning
confidence: 99%
“…Murine BM cells were collected and quantified as previously described (26). Murine BM cells were cultured in murine 20 ng/ml thrombopoietin, 125 ng/ml stem cell factor, and 50 ng/ml Flt-3 ligand (TSF, R&D Systems, Minneapolis, MN).…”
Section: Hematopoietic Progenitor Cell Assays and Transplantation Assmentioning
confidence: 99%