Epidermal growth factor‐containing wound closure enhances wound healing in non‐diabetic and diabetic rats

Doğan, Salih; Demirer, Seher; Kepenekçi, İlknur; Erkek, B.; Kızıltay, Aysel; Hasırcı, Nesrin; Müftüoğlu, Sevda; Nazikoglu, Aysegul; Renda, Nurten; Dinçer, Ü. Deniz; Elhan, Atilla Halil; Kuterdem, Ercüment

doi:10.1111/j.1742-481x.2009.00584.x

Cited by 57 publications

(41 citation statements)

References 41 publications

(61 reference statements)

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“…It has been suggested that the various phases of the healing process are controlled in large part by growth factors, such as, transforming growth factor‐β, insulin‐like growth factor, platelet‐derived growth factor, EGF, VEGF, and fibroblast growth factor, which are released by various cell types in the vicinity of wounds 18, 19. The direct applications of growth stimulating polypeptides to wounds have been reported to improved the rate of healing of chronic wounds 20–22. In addition, the use of topical erythropoietin on wounds in diabetic rats was shown to be successful 23, 24…”

Section: Discussionmentioning

confidence: 99%

Topical embryonic stem cells enhance wound healing in diabetic rats

Lee

Choi

Cho

et al. 2011

Journal Orthopaedic Research

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The effects of embryonic stem cells (ESCs) on diabetic wound healing were investigated using an excisional skin wound model in 110 diabetes-induced rats. We transplanted a clonal population of ESCs (5 Â 10 6 ) by topical injection into full thickness skin wounds. Four study groups were used; nondiabetic rats as a control, non-insulin controlled diabetic rats not treated with ESCs, insulin controlled diabetic rats not treated with ESCs, and insulin controlled diabetic rats treated with ESCs. Five rats in each experimental group were sacrificed on days 1, 5, 10, 15, and 20 after wounding. Wounds images were acquired daily and wound sizes were calculated. We measured the mRNA levels of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF), and fibronectin levels in extracellular matrix, and assessed wound healing by assessing histological parameters of epidermal regeneration, granulation tissue thickness, and angiogenesis. In the ESC-treated group, wound sizes were significantly smaller than in the insulin controlled diabetic group not treated with ESCs on days 5 and 10 (p < 0.05), and EGF and VEGF levels were markedly higher on days 5 and 10, fibronectin levels on day 5 after injection. All histological scores in the ESC-treated group were significantly higher than those of the insulin controlled diabetic group on day 5 (p < 0.05). Our results shows that topical ESCs enhance diabetic wound healing during the early stage, and suggest that ESCs transplantation offers a novel therapeutic modality for the treatment of diabetic wounds. ß

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Section: Discussionmentioning

confidence: 99%

Topical embryonic stem cells enhance wound healing in diabetic rats

Lee

Choi

Cho

et al. 2011

Journal Orthopaedic Research

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“…On the other hand, gelatin is a protein derived from collagen and contains Arg–Gly–Asp (RGD) sequences found in the ECM, therefore it promotes initial cell attachment and increases cell spreading, even more than chitosan (Lien et al , 2009). It is used in supporting soft tissues, such as wound‐healing materials (Dogan et al , 2009), and in the formation of nano‐carriers for chemotherapeutic drugs (Muvaffak et al , 2004; Ofokansi et al , 2010). At physiological pH, gelatin has an anionic and chitosan an cationic nature, so that electrostatic attractions between these charged polymers can easily form scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Development of porous chitosan-gelatin/hydroxyapatite composite scaffolds for hard tissue-engineering applications

Isikli

Hasırcı

2011

J Tissue Eng Regen Med

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Composite scaffolds prepared from natural polymers and hydroxyapatite (HA) are expected to have enhanced osteoconductive properties and as a result gained much attention in recent years for use in bone tissue-engineering applications. Although there are various natural polymers available for this purpose, chitosan (C) and gelatin (G) are commonly studied because of their inherent properties. The aim of this study was to prepare three-dimensional (3D) scaffolds using these two natural polymers and to add either non-sintered hydroxyapatite (nsHA) or sintered hydroxyapatite (sHA) to compare their influence on physical, chemical and mechanical properties of the scaffolds and on their affinities towards Saos-2 cells. For this purpose, nsHA and sHA were synthesized and characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and particle size analyses. Then nsHA and sHA particles, with average sizes of 16 µm and 6 µm, respectively, were added to the solutions of C and G during the preparation step and the resultant 3D scaffolds were characterized. Compression tests indicated that presence of nsHA or sHA increased the Young's modulus and compressive strength of the scaffolds, and the values were very similar to those of human spongy bone. MTS assays, confocal microscopy and SEM analysis showed that cell attachment and proliferation were higher on C-G/sHA composite scaffolds compared to the other scaffolds. It was shown that the scaffolds prepared from chitosan, gelatin and HA are appropriate cell carriers for bone tissue engineering, especially those with sHA incorporated.

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“…To our knowledge, no reports on the effect of pitavastatin on keratinocyte migration have been published. EGF, which is well known to be effective in wound healing, 14 significantly induced migration in HaCaT cells and increased migration, with a tendency toward significance, in NHKs, compared with untreated cells. Treatment with neither pitavastatin nor pitavastatin together with LDL resulted in any significant difference in HaCaT cell or NHK migratory activity compared with DMSO‐treated and untreated cells.…”

Section: Discussionmentioning

confidence: 89%

Effects of a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor and low-density lipoprotein on proliferation and migration of keratinocytes

El-latif

Murota

Terao

et al. 2010

British Journal of Dermatology

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Epidermal growth factor‐containing wound closure enhances wound healing in non‐diabetic and diabetic rats

Abstract: These findings implicate that use of EGF in gelatin-microsphere dressings improves wound healing both in normal and diabetic rats.

Cited by 57 publications

References 41 publications

Topical embryonic stem cells enhance wound healing in diabetic rats

Topical embryonic stem cells enhance wound healing in diabetic rats

Development of porous chitosan-gelatin/hydroxyapatite composite scaffolds for hard tissue-engineering applications

Effects of a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor and low-density lipoprotein on proliferation and migration of keratinocytes

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