Topical embryonic stem cells enhance wound healing in diabetic rats

Abstract

The effects of embryonic stem cells (ESCs) on diabetic wound healing were investigated using an excisional skin wound model in 110 diabetes-induced rats. We transplanted a clonal population of ESCs (5 Â 10 6 ) by topical injection into full thickness skin wounds. Four study groups were used; nondiabetic rats as a control, non-insulin controlled diabetic rats not treated with ESCs, insulin controlled diabetic rats not treated with ESCs, and insulin controlled diabetic rats treated with ESCs. Five rats in each experimental group were sacrificed on days 1, 5, 10, 15, and 20 after wounding. Wounds images were acquired daily and wound sizes were calculated. We measured the mRNA levels of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF), and fibronectin levels in extracellular matrix, and assessed wound healing by assessing histological parameters of epidermal regeneration, granulation tissue thickness, and angiogenesis. In the ESC-treated group, wound sizes were significantly smaller than in the insulin controlled diabetic group not treated with ESCs on days 5 and 10 (p < 0.05), and EGF and VEGF levels were markedly higher on days 5 and 10, fibronectin levels on day 5 after injection. All histological scores in the ESC-treated group were significantly higher than those of the insulin controlled diabetic group on day 5 (p < 0.05). Our results shows that topical ESCs enhance diabetic wound healing during the early stage, and suggest that ESCs transplantation offers a novel therapeutic modality for the treatment of diabetic wounds. ß

Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice

“…Previous studies reported that the MSCs migrate into the wound area [20] and differentiate into keratinocytes, endothelial cells, sweat glands, sebaceous glands and hair follicles [16,21,22]. Additionally, several studies have shown that MSCs secrete paracrine factors, such as VEGF, bFGF, epidermal growth factor, keratinocyte growth factor, insulin-like growth factor and hepatocyte growth factor [16,18,21,[23][24][25] and stimulate the deposition of extracellular matrix [26].…”

Skin Wound Healing: An Update on the Current Knowledge and Concepts

“…Stem cells or progenitor cells seem to support this process by multiple paracrine effects especially by high levels of pro-angiogenic molecules (i.e., VEGF, HGF, bFGF, EGF, TGF-β, IGF-1) [44,45,46,47]. These effects could be demonstrated in rodent diabetic wounding models, further underscoring the significant activity of stem cells and their potential in repair-resistant chronic wounds [45,46,48,49]. …”

Reactive oxygen species induce MMP12‐dependent degradation of collagen 5 and fibronectin to promote the motility of human umbilical cord‐derived mesenchymal stem cells

“…Mouse skin wounding and stem cell implantation were performed as described previously (Lee et al, 2011;Kishibe et al, 2012). Briefly, after their backs had been shaved and treated with an organic iodine solution, a circular fullthickness wound was surgically created using an 8 mm diameter sterile biopsy punch.…”