Epidermal growth factor released in human dental pulp following orthodontic force

Abstract

This study investigated the role of human epidermal growth factor (EGF) in the angiogenic response of the dental pulp to orthodontic force. The release of angiogenic growth factor EGF in human dental pulp following orthodontic force application was examined using neutralizing antibody anti-human (anti-h) EGF to block its effects. The dental pulps from 10 premolar teeth from 10 patients (equal numbers of males and females aged 11-14 years), treated with a straightwire fixed appliance for 2 weeks and extracted for orthodontic reasons, were divided vertically, and sections from each half-pulp were individually co-cultured with a section of rat aorta in collagen surrounded by growth media. Anti-h EGF was added to the media of the co-cultures from one-half of each pulp from each tooth from each patient; the remaining co-cultures from the other half of each pulp without anti-h EGF were used as the controls. Cultures were examined daily by light microscopy for angiogenic growth and number of microvessels. The addition of anti-h EGF to the growth media in the co-cultures resulted in a significant reduction (P < 0.05, Wilcoxon signed rank test) in pulpal and rat aorta microvessel numbers, compared with the control co-cultures. The results indicate that EGF released following orthodontic force application plays a part in the angiogenic response of the pulp.

A histological and micro-CT investigation in to the effect of NGF and EGF on the periodontal, alveolar bone, root and pulpal healing of replanted molars in a rat model - a pilot study

“…EGF is known to promote angiogenesis in vivo [40] and EGF receptors have been localised in the dental pulp in the rat [41]. Derringer and Linden have shown that the addition of anti-h EGF to pulp cell culture reduced the angiogenic response with significantly fewer micro-vessel formations [42]. Therefore, we hypothesise that the addition of EGF may have accelerated pulpal revascularisation after dental replantation and induced earlier pulpal healing.…”

Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma

“…cytic cells through NK1 and makes them release proinflammatory cytokines that have angiogenic and angiostatic functions. Table 2 summarizes the action of some of these cytokines, including the vascular endothelial growth factor (VEGF), the basic fibroblastic growth factor (bFGF), the transforming growth factor-beta (TGF-b), angiogenin, leptin, angiopoietin 2 (ANG-2), the epidermal growth factor (EGF), the heparin-binding epidermal growth factor (HB-EGF) and the platelet-derived growth factor (PDGF) (Derringer & Linden 2007, Mattuella et al 2007a,b, El Karim et al 2009, Kohara et al 2010, Virtej et al 2013. Vascular endothelial growth factor is a heparinbinding protein with specific affinity to endothelial cells and plays a key role in angiogenesis.…”

“…Angiogenesis is affected by the concentration of neuropeptides as the angiogenic phenomenon produced by SP is dose dependent. In other words, the more intense and prolonged the occlusal trauma, the higher the SP release, and therefore a greater differentiation and proliferation of the vascular endothelium will occur (Derringer & Linden 2007, Caviedes-Bucheli et al 2008, 2011a, Kohara et al 2010).…”

Gene expression of growth factors with angiogenic potential in human dental pulp tissue from teeth with complete and incomplete root development

“…Angiogenesis is a complex and highly regulated process that forms blood vessels from pre-existing ones and requires the activation, proliferation, migration, invasion and sprouting of endothelial cells, as well as basement membrane formation and maturation of neovascularization. These processes are mediated by growth factors such as vascular endothelial growth factor (VEGFA), basic fibroblastic growth factor (bFGF), transforming growth factor beta (TGFß), angiogenin, angiopoietin 2 (ANG-2), epidermal growth factor (EGF), heparin-binding epidermal growth factor (HB-EGF) and platelet-derived growth factor (PDGF) amongst others (Derringer & Linden 2007, Mattuella et al 2007, El Karim et al 2009, Virtej et al 2013, Caviedes-Bucheli et al 2017.…”