Epidermolytic toxin serotype B of<i>Staphylococcus aureus</i>is plasmid-encoded

O’Toole, Paul W.; Foster, Timothy J.

doi:10.1111/j.1574-6968.1986.tb01716.x

Cited by 20 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They caused equally general exfoliation of the epidermis accompanied by the so-called Nikolsky sign when inoculated into neonatal mice, but they differ in amino acid composition, amino acid sequence, and heat resistance [2,6,15]. ETA gene (eta gene) is located on the chromosomal DNA of S. aureus, whereas ETB gene (etb gene) is plasmidencoded [4,8,[12][13][14]19]. In addition, a new type of exfoliative toxin was purified from a S. aureus strain isolated from a horse with phlegmon and was designated exfoliative toxin C (ETC) [18].…”

mentioning

confidence: 99%

Production of Exfoliative Toxin A by Staphylococcus aureus Isolated from Mastitic Cow's Milk and Farm Bulk Milk.

Hayakawa

Hayashi

Shimano

et al. 1998

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The production of exfoliative toxins A and B (ETA and ETB) by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk was examined by the reverse passive latex agglutination method (RPLA). ETA was detected in 2 (1.2%) of 162 isolates from mastitic cow's milk and in 1 (0.6%) of 166 isolates from farm bulk milk. RPLA titers of these isolates were much lower than in human isolates. No ETB was detected in any of the isolates tested. These ETA-positive isolates belonged to bovine ecovar. They were non-typable using the international phage set for human strains. When these ETA-positive isolates were subcutaneously inoculated into neonatal mice, general exfoliation of the epidermis accompanied by the so-called Nikolsky sign was not recognized. By the immunoblotting and PCR methods, however, ETA and eta gene were recognized in the ETA-positive isolates from mastitic cow's milk and farm bulk milk. These data suggest that ETA is also produced by bovine isolates of S. aureus, but in smaller quantities. -KEY WORDS: bovine mastitis, exfoliative toxin A, Staphylococcus aureus.J. Vet. Med. Sci. 60(11): 1281-1283, 1998 farm bulk milk, respectively, obtained at 71 dairy farms in Ishikawa Prefecture during the period from 1991 to 1994. Six human strains of S. aureus, isolated from patients affected with staphylococcal scalded-skin syndrome, were used as ETA and ETB positive controls. The organisms were inoculated into 5 ml of brain heart infusion (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) and cultured at 37°C for 18 hr. The culture fluids were centrifuged at 12,000 × g at 4°C for 5 min and then the resulting culture supernatants were tested for ETA and ETB by a reverse passive latex agglutination (RPLA) test using EXT-RPLA kit (Denka Seiken Co., Ltd., Tokyo, Japan). Briefly, 20 µl of the culture supernatants, diluted 5-fold, were placed into the wells of V-type microtiter plates. An equal volume of latex particles, sensitized with specific anti-ETA or anti-ETB immunoglobulins, was added to each well of the plates. After thorough mixing, the plates were incubated at room temperature for 24 hr. When the aggulutinating reaction was obtained, the culture supernatants of the positive isolates were serially diluted twofold in microtiter plates to determine the quantity of ETA or ETB.

show abstract

mentioning

confidence: 99%

Production of Exfoliative Toxin A by Staphylococcus aureus Isolated from Mastitic Cow's Milk and Farm Bulk Milk.

Hayakawa

Hayashi

Shimano

et al. 1998

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

Staphylococcus

Peacock

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

View full text Add to dashboard Cite

Multiplex PCR for detection of three exfoliative toxin serotype genes inStaphylococcus aureus

et al. 2005

View full text Add to dashboard Cite

Rapid and specific detection of exfoliative toxin (ET)-producing Staphylococcus aureus strains by multiplex polymerase chain reaction (PCR) was used for identification of exfoliative toxin genes in a diverse set of 115 clinical S. aureus strains isolated in 14 Czech cities between 1998 and 2004. Fifty-nine wild-type ET-positive isolates of which 40 strains were the causative agents of toxic epidermolysis in neonates were classified into 4 PCR types. The genes coding for ETA, ETB or ETD were not detected in any of non-ET-producing isolates. The PCR method using the multiplex and specific primer set was shown to be reliable in rapid identification of the exfoliative toxin producing S. aureus and can be used as a convenient tool for hospital epidermolytic infection control.

show abstract

Epidermolytic toxin serotype B ofStaphylococcus aureusis plasmid-encoded

Cited by 20 publications

References 15 publications

Production of Exfoliative Toxin A by Staphylococcus aureus Isolated from Mastitic Cow's Milk and Farm Bulk Milk.

Production of Exfoliative Toxin A by Staphylococcus aureus Isolated from Mastitic Cow's Milk and Farm Bulk Milk.

Staphylococcus

Multiplex PCR for detection of three exfoliative toxin serotype genes inStaphylococcus aureus

Contact Info

Product

Resources

About