Epigallocatechin-3-gallate added after thawing to frozen dog semen: Effect on sperm parameters and ability to bind to oocytes’ zona pellucida

Bucci, Diego; Cunto, Marco; Gadani, Beatrice; Spinaci, Marcella; Zambelli, Daniele; Galeati, Giovanna

doi:10.1016/j.repbio.2018.12.001

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…shown that the addition of EGCG can improve the motility and acrosome integrity of dog sperm, thereby improving the fertilisation ability of sperm (Bucci et al, 2019). Adding EGCG to improve sperm kinematics parameters may be due to the reduction of bacterial contamination caused by air exposure (Tsou et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Sperm motility is an important factor that affects subsequent insemination, and the integrity of the acrosome is also the key to whether the sperm can dissolve the radioactive crown and the transparent zone and successfully enter the ovum. Previous studies have shown that the addition of EGCG can improve the motility and acrosome integrity of dog sperm, thereby improving the fertilisation ability of sperm (Bucci et al, 2019). Adding EGCG to improve sperm kinematics parameters may be due to the reduction of bacterial contamination caused by air exposure (Tsou et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…As an antioxidant, it was recently observed that the addition of EGCG could improve the fertilisation efficiency of boar sperm by improving the anti‐oxidant capacity (Gadani et al, 2017). In addition to its role in the cryopreservation of boar semen, the addition of EGCG was found to significantly improve sperm motility and acrosomal integrity when freezing dog semen (Bucci et al, 2019). Similarly, EGCG has been found to improve cryopreservation of buffalo semen in relation to sperm movement and the content of various antioxidant enzymes (Ahmed et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Epigallocatechin 3‐gallate improves the quality of bull semen cryopreservation

Wang

Yuan

et al. 2021

Andrologia

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To determine the effects of epigallocatechin-3-gallate (EGCG) on the cryopreservation of bovine semen, epigallocatechin-3-gallate dissolved with double distilled water to 0.2, 0.4, 0.6 and 0.8 mg/ml were added to the cryopreservation diluent of the bull semen. Then, we used computer-assisted analysis of semen kinematic parameters, staining method to detect membrane function, acrosome integrity, enzyme-linked immunosorbent assay to detect catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehydes (MDA) and reactive oxygen levels.The results showed that adding 0.6 mg/L of epigallocatechin-3-gallate could improve the cryopreserved sperm quality, which significantly increased the total motility, distance average path, distance straight line, distance curved line, average path velocity, curvilinear-velocity, straight-line velocity, amplitude of lateral head displacement and beat/cross frequency, as well as sperm CAT, GSH-Px and SOD levels (p < 0.05), whilst reducing the reactive oxygen species and MDA levels (p < 0.05). Hence, these results indicate that the addition of 0.6 mg/ml of EGCG has a protective effect on the cryopreservation of the bovine semen.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Epigallocatechin 3‐gallate improves the quality of bull semen cryopreservation

Wang

Yuan

et al. 2021

Andrologia

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“…, addition of prostatic fluid after thawing may be appropriate to improve sperm motility [ 67 , 68 , 69 ]. In addition, sperm motility is reportedly improved by post-thawing addition of pentoxifylline [ 70 , 71 , 72 ], epigallocatechin-3-gallate [ 73 ], caffeine [ 71 , 72 ], and 2'-deoxyadenosine [ 71 , 72 ]. However, the fertility of treated spermatozoa after artificial insemination has not yet been assessed.…”

Section: Extender For Canine Spermatozoamentioning

confidence: 99%

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

2022

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Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. Their widespread use has contributed to improvements in human health and welfare. However, in dogs, only artificial insemination using frozen semen is readily available as an ART to improve breeding and control genetic diversity. A recent priority in sperm cryopreservation is the development of alternatives to egg yolk, which is widely used as a component of the sperm extender. Egg yolk can vary in composition among batches and is prone to contamination by animal pathogens. The latter can be a problem for international exchange of cryopreserved semen. Low-density lipoprotein and skim milk are promising candidates for use as extenders, to ensure fertility after artificial insemination. Although not tested for its effects on fertility following artificial insemination, polyvinyl alcohol may also be a useful alternative to egg yolk as an extender. The development of cryopreservation techniques for canine embryos lags behind that for other mammals, including humans. However, given the success of non-surgical embryo transfer in 2011, studies have sought to refine this approach for practical use. Research on sperm cryopreservation has yielded satisfactory results. However, investigation of other approaches, such as cryopreservation of oocytes and gonadal tissues, remains insufficient. Techniques for the efficient induction of estrus may aid in the development of successful canine ARTs.

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“…8,9 However, a recent study by Bucci et al demonstrated that EGCG supplementation to thawing medium failed to improve dog sperm quality or zona binding capacity. 10 As far as we know, there is still a lack of systematic research on the effects of EGCG on the IVM and IVF of porcine oocytes. Whether EGCG can be used as an effective antioxidant for porcine oocytes cultured in vitro remains to be explored.…”

Section: Introductionmentioning

confidence: 99%