(‐)‐Epigallocatechin‐3‐gallate Ameliorates Insulin Resistance and Mitochondrial Dysfunction in HepG2 Cells: Involvement of Bmal1

Mi, Yashi; Qi, Guoyuan; Gao, Yuqi; Li, Runnan; Wang, Yiwen; Li, Xingyu; Huang, Shuxian; Liu, Xuebo

doi:10.1002/mnfr.201700440

Cited by 47 publications

(46 citation statements)

References 58 publications

(95 reference statements)

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“…Hyperglycemia-mediated mitochondrial superoxide overproduction can suppress the activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and it meanwhile leads to the activation of the hexosamine pathway with the increasing influx of phosphorylated glucose [ 87 , 88 ]. High glucosamine generated by the activation of hexosamine pathway stimulates the overproduction of ROS in mitochondria and damages mitochondrial respiration [ [89] , [90] , [91] ], which can further aggravate oxidative stress, augment vascular permeability, and promote angiogenesis.…”

Section: Oxidative Stress and The Abnormalities Of Polyol/pkc/age Formentioning

confidence: 99%

Oxidative stress and diabetic retinopathy: Molecular mechanisms, pathogenetic role and therapeutic implications

2020

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Oxidative stress, a cytopathic outcome of excessive generation of ROS and the repression of antioxidant defense system for ROS elimination, is involved in the pathogenesis of multiple diseases, including diabetes and its complications. Retinopathy, a microvascular complication of diabetes, is the primary cause of acquired blindness in diabetic patients. Oxidative stress has been verified as one critical contributor to the pathogenesis of diabetic retinopathy. Oxidative stress can both contribute to and result from the metabolic abnormalities induced by hyperglycemia, mainly including the increased flux of the polyol pathway and hexosamine pathway, the hyper-activation of protein kinase C (PKC) isoforms, and the accumulation of advanced glycation end products (AGEs). Moreover, the repression of the antioxidant defense system by hyperglycemia-mediated epigenetic modification also leads to the imbalance between the scavenging and production of ROS. Excessive accumulation of ROS induces mitochondrial damage, cellular apoptosis, inflammation, lipid peroxidation, and structural and functional alterations in retina. Therefore, it is important to understand and elucidate the oxidative stress-related mechanisms underlying the progress of diabetic retinopathy. In addition, the abnormalities correlated with oxidative stress provide multiple potential therapeutic targets to develop safe and effective treatments for diabetic retinopathy. Here, we also summarized the main antioxidant therapeutic strategies to control this disease.

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Section: Oxidative Stress and The Abnormalities Of Polyol/pkc/age Formentioning

confidence: 99%

Oxidative stress and diabetic retinopathy: Molecular mechanisms, pathogenetic role and therapeutic implications

2020

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“…In addition, the compensatory growth of β-cells caused by diet-induced obesity-related insulin increases is controlled by Bmal1 ( Rakshit et al, 2016 ). These observations suggest that the core circadian gene Bmal1 is involved in fatty acid synthesis and the β-oxidation pathway ( Mi et al, 2017b ). Therefore, regulating circadian rhythm disorders caused by alcohol feeding may be a treatment target for ALD.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, regulating circadian rhythm disorders caused by alcohol feeding may be a treatment target for ALD. The studies have discovered that EGCG and tea polyphenols reduce insulin resistance in a Bmal1-dependent way and by activating AMPK signaling pathways ( Mi et al, 2017a ; Mi et al, 2017b ). Besides, proanthocyanidins can regulate circadian gene expression in cultured fibroblasts ( Ribas-Latre et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Physcion Protects Against Ethanol-Induced Liver Injury by Reprogramming of Circadian Clock

et al. 2020

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The circadian clock plays a key role in our daily physiology and metabolism. Alcohol consumption disrupts the circadian rhythm of metabolic genes in the liver; however, the potential contribution of circadian clock modulation to alcoholic liver disease (ALD) is unknown. We identified a novel liver protective agent, physcion, which can alleviate fat accumulation and inflammation in ALD mice via reprogramming the hepatic circadian clock. The model of alcoholic hepatitis was established by intragastrically administering ethanol. In vitro, physcion was investigated by treating HepG2 cells with ethanol. The role of circadian clock in Physcion caused liver protection was tested by knocking down the core circadian gene Bmal1. Physcion application caused reduced lipogenesis and alleviated inflammation in alcohol-induced mice. In alcoholic hepatosteatosis models, physcion upregulated the core circadian genes. And the circadian misalignment triggered by ethanol was efficiently reversed by physcion. Physcion attenuated lipogenesis via reprogramming the circadian clock in HepG2 cells. Suppression of Bmal1 by RNA interference abolished the protective of physcion. In addition, Physcion binds to the active pocket of BMAL1 and promotes its expression. The study identified the novel liver protective effects of physcion on alcohol-induced liver injury, and modulation of the core circadian clock regulators contributes to ALD alleviation. More importantly, strategies targeting the circadian machinery, for example, Bmal1, may prove to be beneficial treatment options for this condition.

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“…Food components, such as glucose, sodium, amino acids, caffeine, thiamine, and ethanol, are capable of resetting or phase-shifting circadian rhythms [39,40]. Mi et al found that EGCG (a biologically active component in green tea) can regulate glucosamine-induced HepG2 hepatocyte IR via the core circadian protein Bmal1 [23]. In db / db mutant mice, the clock is also obligated for the remitting effects of nobiletin on metabolic disorders [37].…”

Section: Discussionmentioning

confidence: 99%

“…Bioactive components in natural foods, such as proanthocyanidins, (-)-Epigallocatechin-3-gallate (EGCG), chicoric acid, and nobiletin, can manipulate circadian rhythms to ameliorate metabolic syndrome [21,22,23]. Resveratrol (RES, 3,5,4′-trihydroxystilbene), a natural product derived from grapes, has various health benefits, including anti-inflammatory, anti-oxidant, and anti-mutation properties [24].…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Maintains Lipid Metabolism Homeostasis via One of the Mechanisms Associated with the Key Circadian Regulator Bmal1

Wei

Zhao

et al. 2019

Molecules

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Resveratrol (RES) possesses anti-inflammatory and anti-oxidant activities, and it can prevent liver lipid metabolism disorders in obese and diabetic individuals. This study elucidated the mechanisms of brain and muscle Arnt-like protein-1 (Bmal1) in the protective effects of RES against liver lipid metabolism disorders. The results indicated that RES ameliorated free fatty acid (FFA)-induced (oleic acid (OA): palmitic acid (PA) = 2:1) glycolipid metabolic disorders in hepatocytes. Simultaneously, RES partially reverted the relatively shallow daily oscillations of FFA-induced circadian clock gene transcription and protein expression in HepG2 cells. RES also attenuated FFA-triggered reactive oxygen species (ROS) secretion and restored mitochondrial membrane potential consumption, as well as the restoration of mitochondrial respiratory complex expression. This study provides compelling evidence that RES controls intracellular lipid metabolic imbalance in a Bmal1-dependent manner. Overall, RES may serve as a promising natural nutraceutical for the regulation of lipid metabolic disorders relevant to the circadian clock.

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(‐)‐Epigallocatechin‐3‐gallate Ameliorates Insulin Resistance and Mitochondrial Dysfunction in HepG2 Cells: Involvement of Bmal1

Cited by 47 publications

References 58 publications

Oxidative stress and diabetic retinopathy: Molecular mechanisms, pathogenetic role and therapeutic implications

Oxidative stress and diabetic retinopathy: Molecular mechanisms, pathogenetic role and therapeutic implications

Physcion Protects Against Ethanol-Induced Liver Injury by Reprogramming of Circadian Clock

Resveratrol Maintains Lipid Metabolism Homeostasis via One of the Mechanisms Associated with the Key Circadian Regulator Bmal1

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