Epigallocatechin-3-gallate exhibits immunomodulatory effects in human primary T cells

Huang, Shih Chung; Kao, Ying-Jer; Shih, Shao Fu; Tsai, Min Chien; Lin, Chin; Chen, Liv Weichien; Chuang, Yi Ping; Tsui, Pi Fen; Ho, Ling Jun; Lai, Jenn Haung; Chen, Sy‐Jou

doi:10.1016/j.bbrc.2021.02.132

Cited by 21 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consumption of exogenous polyphenols may play an important role in gaining or maintaining immunity by way of interrupting the synthesis of proinflammatory cytokines, thus regulating immune cells gene expression [151,153]. Studies on laboratory animals, humans as well as in vitro have confirmed the positive effects of consuming tea or polyphenols isolated therefrom on organisms with autoimmune diabetes (Tables 1 and 2).…”

Section: Impact Of Tea On Organisms With Autoimmune Diabetes-a Reviewmentioning

confidence: 90%

“…When administered to mice with autoimmune encephalomyelitis, EGCG reduced the clinical symptoms of the disease as well as the pathological immune response [150]. An inhibitive impact of EGCG on the release of inflammatory cytokines was reported in activated human primary T cells, most likely due to inactivation of Ap-1 [151]. In a study on piglets, it was demonstrated that tea polyphenols promote the proliferation of immune cells, T cell activation, increased concentration of CD4+ T lymphocytes, increased values of the CD4+/CD8+ ratio as well as improvement in T cell transformation (LTT) [152].…”

Section: Anti-inflammatory and Immunomodulatory Propertiesmentioning

confidence: 98%

See 1 more Smart Citation

Antioxidant, Anti-Inflammatory, and Immunomodulatory Properties of Tea—The Positive Impact of Tea Consumption on Patients with Autoimmune Diabetes

2021

View full text Add to dashboard Cite

The physiological markers of autoimmune diabetes include functional disorders of the antioxidative system as well as progressing inflammation and the presence of autoantibodies. Even though people with type 1 diabetes show genetic predispositions facilitating the onset of the disease, it is believed that dietary factors can stimulate the initiation and progression of the disease. This paper analyses the possibility of using tea as an element of diet therapy in the treatment of type 1 diabetes. Based on information available in literature covering the last 10 years, the impact of regular tea consumption or diet supplements containing tea polyphenols on the oxidative status as well as inflammatory and autoimmune response of the organism was analyzed. Studies conducted on laboratory animals, human patients, and in vitro revealed positive effects of the consumption of tea or polyphenols isolated therefrom on the diabetic body. Few reports available in the literature pertain to the impact of tea on organisms affected by type 1 diabetes as most (over 85%) have focused on cases of type 2 diabetes. It has been concluded that by introducing tea into the diet, it is possible to alleviate some of the consequences of oxidative stress and inflammation, thus limiting their destructive impact on the patients’ organisms, consequently improving their quality of life, regardless of the type of diabetes. Furthermore, elimination of inflammation should reduce the incidence of immune response. One should consider more widespread promotion of tea consumption by individuals genetically predisposed to diabetes, especially considering the drink’s low price, easy availability, overall benefits to human health, and above all, the fact that it can be safely used over extended periods of time, regardless of the patient’s age.

show abstract

Section: Impact Of Tea On Organisms With Autoimmune Diabetes-a Reviewmentioning

confidence: 90%

Section: Anti-inflammatory and Immunomodulatory Propertiesmentioning

confidence: 98%

Antioxidant, Anti-Inflammatory, and Immunomodulatory Properties of Tea—The Positive Impact of Tea Consumption on Patients with Autoimmune Diabetes

2021

View full text Add to dashboard Cite

show abstract

“…Additionally, the anti-inflammatory properties of EGCG, particularly on human primary T cells, are well-documented. A recent study by Huang et al (2021) [53] showed that EGCG regulates IL-2 and TNF-alpha levels, while Th1 cells producing inflammatory cytokines, TNF-alpha, and IL-2 showed that they can be inhibited during inflammation. According to the findings, EGCG has the ability to effectively decrease the release of cytokines by activated human primary T cells.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Antioxidant, Immunomodulatory Activity of Oxidised Epigallocatechin-3-Gallate (Green Tea Polyphenol) and Its Action on the Main Protease of SARS-CoV-2—An In Vitro and In Silico Approach

et al. 2022

View full text Add to dashboard Cite

Owing to the instability of Epigallocatechin Gallate (EGCG), it may undergo auto-oxidation and form oxidised products or dimers. In the present study, we aimed to evaluate the therapeutic effects, including antioxidation and immunomodulatory action, of the Oxidised Epigallocatechin Gallate (O-EGCG) as compared to native EGCG and the action of these compounds on main protease (Mpro) docking against SARS-CoV-2. HCT-116 (Human Colon Cancer) cell lines were used to estimate the total antioxidant capacity and lipid peroxidation levels and pro-inflammatory markers (human IL-6, IL-1β, TNF-α). Further, molecular docking analysis was performed by AutoDock and visualised in Discovery studio. Improved antioxidant capacity of O-EGCG was observed, and there was a significant decrease in the inflammatory markers (IL-1β, IL-6, and TNF-α) when O-EGCG was applied as compared to EGCG. The O-EGCG was shown to be strongly associated with the highest docking score and active site residues of IL-1, IL-6, and TNF- α, as well as the Mpro of SARS-CoV-2, according to in silico approach. The in vitro and in silico analyses indicate an improved therapeutic action of the oxidised form of EGCG. The effective inhibitory action of O-EGCG against SARS-CoV-2 suggests further exploration of the compound against COVID-19 and its efficacy. However, in vivo studies and understanding of the mechanism of action of O-EGCG may yield a better opinion on the use of O-EGCG and future human clinical trials.

show abstract

“…In 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease (PD) mouse model, EGCG could increase the ratio of CD3+CD4+ to CD3+CD8+ T lymphocytes and modulate peripheral immune response [ 42 ]. In human primary T cells, EGCG (10 μM or 20 μM) tended to inactivate AP-1 DNA-binding activity, and decreased INF-γ levels by 31.3% and 34.7%, IL-2 levels by 26.0% and 38.8%, IL-4 levels by 41.5% and 55.9%, as well as TNF-α levels by 23.0% and 37.6%, respectively [ 43 ]. Moreover, the level of p-JNK and p-ERK was also decreased by EGCG, but showing no effects to levels of p-p38 MAPK and total protein amounts [ 43 ].…”

Section: Immune Potentiating Effects Of Tea Polyphenolsmentioning

confidence: 99%

“…In human primary T cells, EGCG (10 μM or 20 μM) tended to inactivate AP-1 DNA-binding activity, and decreased INF-γ levels by 31.3% and 34.7%, IL-2 levels by 26.0% and 38.8%, IL-4 levels by 41.5% and 55.9%, as well as TNF-α levels by 23.0% and 37.6%, respectively [ 43 ]. Moreover, the level of p-JNK and p-ERK was also decreased by EGCG, but showing no effects to levels of p-p38 MAPK and total protein amounts [ 43 ].…”

Section: Immune Potentiating Effects Of Tea Polyphenolsmentioning

confidence: 99%

Immunomodulatory Effects of Green Tea Polyphenols

Wang

et al. 2021

Molecules

View full text Add to dashboard Cite

Green tea and its bioactive components, especially polyphenols, possess many health-promoting and disease-preventing benefits, especially anti-inflammatory, antioxidant, anticancer, and metabolic modulation effects with multi-target modes of action. However, the effect of tea polyphenols on immune function has not been well studied. Moreover, the underlying cellular and molecular mechanisms mediating immunoregulation are not well understood. This review summarizes the recent studies on the immune-potentiating effects and corresponding mechanisms of tea polyphenols, especially the main components of (–)-epigallocatechin-3-gallate (EGCG) and (–)-epicatechin-3-gallate (ECG). In addition, the benefits towards immune-related diseases, such as autoimmune diseases, cutaneous-related immune diseases, and obesity-related immune diseases, have been discussed.

show abstract

Epigallocatechin-3-gallate exhibits immunomodulatory effects in human primary T cells

Cited by 21 publications

References 34 publications

Antioxidant, Anti-Inflammatory, and Immunomodulatory Properties of Tea—The Positive Impact of Tea Consumption on Patients with Autoimmune Diabetes

Antioxidant, Anti-Inflammatory, and Immunomodulatory Properties of Tea—The Positive Impact of Tea Consumption on Patients with Autoimmune Diabetes

Assessment of Antioxidant, Immunomodulatory Activity of Oxidised Epigallocatechin-3-Gallate (Green Tea Polyphenol) and Its Action on the Main Protease of SARS-CoV-2—An In Vitro and In Silico Approach

Immunomodulatory Effects of Green Tea Polyphenols

Contact Info

Product

Resources

About