(−)-Epigallocatechin-3-gallate inhibits entry of hepatitis B virus into hepatocytes

Huang, Haoping; Tao, Mi Hua; Hung, Tzu Min; Chen, Jui Chieh; Lin, Zi; Huang, Cheng

doi:10.1016/j.antiviral.2014.09.009

Cited by 126 publications

(93 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyphenols have also demonstrated potential antibacterial, antifungal, and antiviral activities. For instances, the antiviral effect of epigallocatechin gallate (EGCG), the most abundant polyphenol in green tea, has been already reported for several viruses: hepatitis C virus, chikungunya virus, hepatitis B virus, herpes simplex virus type 1 virus, influenza A virus, vaccinia virus, adenovirus, reovirus, vesicular stomatitis virus, and, very recently, for ZIKV (Ciesek et al, 2011; Calland et al, 2012, 2015; Huang et al, 2014; Weber et al, 2015; Carneiro et al, 2016). Likewise, other polyphenols have also been described to present antiviral activity, such as honokiol, baicalein, naringin, and quercetin (Zandi et al, 2011, 2012; Johari et al, 2012; Fang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Antiviral Properties of the Natural Polyphenols Delphinidin and Epigallocatechin Gallate against the Flaviviruses West Nile Virus, Zika Virus, and Dengue Virus

et al. 2017

View full text Add to dashboard Cite

The Flavivirus genus contains important pathogens, such as West Nile virus (WNV), Zika virus (ZIKV), and Dengue virus (DENV), which are enveloped plus-strand RNA viruses transmitted by mosquitoes and constitute a worrisome threat to global human and animal health. Currently no licensed drugs against them are available, being, thus, still necessary the search for effective antiviral molecules. In this line, a novel antiviral approach (economical, simple to use, and environmental friendly) is the use of natural compounds. Consequently, we have tested the antiviral potential of different polyphenols present in plants and natural products, such as wine and tea, against WNV, ZIKV, and DENV. So that, we assayed the effect of a panel of structurally related polyphenols [delphinidin (D), cyanidin (Cy), catechin (C), epicatechin (EC), epigallocatechin (EGC), and epigallocatechin gallate (EGCG)] on WNV infection, and found that D and EGCG inhibited more effectively the virus production. Further analysis with both compounds indicated that they mainly affected the attachment and entry steps of the virus life cycle. Moreover, D and EGCG showed a direct effect on WNV particles exerting a virucidal effect. We showed a similar inhibition of viral production of these compounds on WNV variants that differed on acidic pH requirements for viral fusion, indicating that their antiviral activity against WNV is produced by a virucidal effect rather than by an inhibition of pH-dependent viral fusion. Both polyphenols also reduced the infectivity of ZIKV and DENV. Therefore, D and EGCG impair the infectivity in cell culture of these three medically relevant flaviviruses.

show abstract

Section: Introductionmentioning

confidence: 99%

Antiviral Properties of the Natural Polyphenols Delphinidin and Epigallocatechin Gallate against the Flaviviruses West Nile Virus, Zika Virus, and Dengue Virus

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Finally, recent studies identified additional FDA approved drugs as HBV and HDV entry inhibitors which act most likely by interfering with NTCP function [42,43]. Interestingly, some previously identified HCV entry inhibitors also interfere with HBV entry, for instance green tea extract (containing EGCG) [44] and ezetimibe [45]. While their detailed mechanism of action is unknown, it is likely that these molecules also interfere with virus-host cell entry factor interactions as shown for HCV [46,47].…”

Section: Host-targeting Entry Inhibitorsmentioning

confidence: 92%

Host-targeting agents for treatment of hepatitis B virus infection

Baumert

Verrier

Nassal

et al. 2015

Current Opinion in Virology

View full text Add to dashboard Cite

“…Although already known NTCP inhibitors have been reported to inhibit viral infection, there is no report identifying novel compounds that target NTCP and inhibit HBV infection. (Ϫ)-Epigallocatechin-3-gallate (EGCG) and Ro41-5253 decreased cell surface NTCP expression and reduced HBV infection (19,50). Vanitaracin A was shown to have a greater potential to inhibit HBV infection (IC 50 of Ͻ1 M) than these compounds (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Vanitaracin A showed a dose-dependent reduction in the level of secreted HBsAg (Fig. 2C); the 50% inhibitory concentration (IC 50 ) was calculated to be 0.61 Ϯ 0.23 M. This compound was not cytotoxic to primary human hepatocyte cultures, with a 50% cytotoxic concentration (CC 50 ) of Ͼ256 M (Fig. 2D).…”

Section: Vanitaracin a Inhibits Hbv Infectionmentioning

confidence: 99%

A Novel Tricyclic Polyketide, Vanitaracin A, Specifically Inhibits the Entry of Hepatitis B and D Viruses by Targeting Sodium Taurocholate Cotransporting Polypeptide

Kaneko

Watashi

Kamisuki

et al. 2015

J Virol

View full text Add to dashboard Cite

Anti-hepatitis B virus (HBV) drugs are currently limited to nucleos(t)ide analogs (NAs) and interferons. A challenge of drug development is the identification of small molecules thatC hronic hepatitis B virus (HBV) infection, constituting a public health problem, with an estimated 240 million carriers worldwide (1), elevates the risk of development of liver cirrhosis and hepatocellular carcinoma (2). Antiviral agents against HBV include nucleos(t)ide analogs (NAs) and interferons (IFNs), which can achieve significant reductions in HBV loads (3). Although IFN-␣ and its pegylated form (peg-IFN-␣) modulate host immune responses to HBV infection or directly inhibit HBV replication in hepatocytes, these regimens show low tolerability because of serious adverse effects (3, 4). NAs, including lamivudine (LMV), adefovir, entecavir (ETV), tenofovir, and telbivudine, inhibit reverse transcription to suppress HBV replication, but longterm treatment with some of these NAs often results in selection for a significant number of drug-resistant viruses, which decreases treatment efficacy; i.e., the introduction of two substitutions, L180M and M204V, in the polymerase region leads to resistance to LMV, and an additional mutation of either T184, S202, or M250 with the L180M/M204V mutations confers further ETV resistance (5). More notably, it is difficult for the above-mentioned anti-HBV drugs to completely eliminate HBV from infected cells. Future antiviral strategies include multidrug treatment with an existing drug and a new anti-HBV agent. Consequently, there is a

show abstract

(−)-Epigallocatechin-3-gallate inhibits entry of hepatitis B virus into hepatocytes

Cited by 126 publications

References 39 publications

Antiviral Properties of the Natural Polyphenols Delphinidin and Epigallocatechin Gallate against the Flaviviruses West Nile Virus, Zika Virus, and Dengue Virus

Antiviral Properties of the Natural Polyphenols Delphinidin and Epigallocatechin Gallate against the Flaviviruses West Nile Virus, Zika Virus, and Dengue Virus

Host-targeting agents for treatment of hepatitis B virus infection

A Novel Tricyclic Polyketide, Vanitaracin A, Specifically Inhibits the Entry of Hepatitis B and D Viruses by Targeting Sodium Taurocholate Cotransporting Polypeptide

Contact Info

Product

Resources

About