Epigallocatechin-3-gallate loaded-zein nanoparticles: Characterization, stability and associated antioxidant, anti-tyrosinase and sun protection properties

Vale, Everton Pantoja; Morais, Edmilson dos S.; Tavares, Walter de Souza; Sousa, Francisco Fábio Oliveira de

doi:10.1016/j.molliq.2022.119107

Cited by 16 publications

(8 citation statements)

References 43 publications

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“…Corn protein zein, a prolamin found in the endosperm of maize ( Zea mays ), is a biodegradable and biocompatible biopolymer able to entrap and deliver therapeutic molecules [ 12 , 13 , 14 , 15 , 16 , 17 ]. Due to these interesting properties zein is an abundantly renewable resource that has long been applied in food and pharmaceutical industries [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

et al. 2023

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Although cationic surfactants have a remarkable antimicrobial activity, they present an intrinsic toxicity that discourages their usage. In this work novel zein nanoparticles loaded with arginine-phenylalanine-based surfactants are presented. The nanoparticles were loaded with two single polar head (LAM and PNHC12) and two with double amino acid polar head surfactants, arginine-phenylalanine (C12PAM, PANHC12). The formulations were characterized and their stability checked up to 365 days. Furthermore, the antimicrobial and hemolytic activities were investigated. Finally, NMR and molecular docking studies were carried out to elucidate the possible interaction mechanisms of surfactant-zein. The nanoparticles were obtained with satisfactory size, zeta potential and dispersibility. The surfactants containing arginine-phenylalanine residues were found to be more stable. The nanoencapsulation maintained the antimicrobial activities unaltered in comparison to the surfactants’ solutions. These results are in agreement with the NMR and docking findings, suggesting that zein interacts with the surfactants by the aromatic rings of phenylalanine. As a result, the cationic charges and part of the aliphatic chains are freely available to attack the bacteria and fungi, while not available to disrupt the cellular membranes. This approach opens new possibilities for using cationic surfactants and benefits from their extraordinary antimicrobial responses for several applications.

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Section: Introductionmentioning

confidence: 99%

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

et al. 2023

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“…One more signal around 1404 cm −1 may be associated with stretching of the C=O bond. A band is observed from 1308 cm −1 to 1232 cm −1 due to hydrogen bonds [ 27 ]. Finally, a signal is observed at 1068 cm −1 , indicating the presence of C-O-C stretching of ester groups [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previous results reported this effect and attributed the signal shift to the formation of hydrogen bridges and hydrophobic interactions. The above-mentioned elucidates a stable system’s formation through non-covalent interactions [ 27 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previously, the possible mechanism of action of phenols was reported: they can act by interrupting the lipid oxidation reaction, inhibiting chemiluminescence reactions by capturing several reactive oxygen species [ 47 ]. In addition, the encapsulation of these extracts favors stability and protection against external factors such as contact with oxygen, temperature, and light, which cause their degradation [ 27 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…Another attractive composite is epigallocatechin-3-gallate-loaded zein nanoparticles. It has been used for its biological properties related to skin pigmentation and sun protection [ 27 ]. Otherwise, a green reductant like an aqueous extract of Citrus sinensis in Ag nanoparticle synthesis can create a capping without any toxic reagent in the formulation.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction

et al. 2022

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In the present research, an orange extract (OE) was obtained and encapsulated in a zein matrix for its subsequent physicochemical characterization and evaluation of its antioxidant capacity. The OE consists of phenolic compounds and flavonoids extracted from orange peel (Citrus sinensis) by ultrasound-assisted extraction (UAE). The results obtained by dynamic light scattering (DLS) and scanning electron microscopy (SEM) indicated that zein nanoparticles with orange extract (NpZOE) presented a nanometric size and spherical shape, presenting a hydrodynamic diameter of 159.26 ± 5.96 nm. Furthermore, ζ-potential evolution and Fourier transform infrared spectroscopy (FTIR) techniques were used to evaluate the interaction between zein and OE. Regarding antioxidant activity, ABTS and DPPH assays indicated no significant differences at high concentrations of orange peel extract and NpZOE; however, NpZOE was more effective at low concentrations. Although this indicates that ultrasonication as an extraction method effectively obtains the phenolic compounds present in orange peels, the nanoprecipitation method under the conditions used allowed us to obtain particles in the nanometric range with positive ζ-potential. On the other hand, the antioxidant capacity analysis indicated a high antioxidant capacity of both OE and the NpZOE. This study presents the possibility of obtaining orange extracts by ultrasound and coupling them to zein-based nanoparticulate systems to be applied as biomedical materials functionalized with antioxidant substances of pharmaceutical utility.

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Soft Nanomaterials and Their Applications

Dua,

Ahmed,

Arfin

2023

Smart Nanomaterials Technology

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Epigallocatechin-3-gallate loaded-zein nanoparticles: Characterization, stability and associated antioxidant, anti-tyrosinase and sun protection properties

Cited by 16 publications

References 43 publications

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

Zein Nanoparticles Containing Arginine-Phenylalanine-Based Surfactants: Stability, Antimicrobial and Hemolytic Activity

Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction

Soft Nanomaterials and Their Applications

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