(–)-Epigallocatechin-3-Gallate Protects Human Skin Fibroblasts from Ultraviolet a Induced Photoaging

Abstract

Background Ultraviolet (UV) is a common stressor of skin and repeated UVA radiation contributes to photoaging. (–)-Epigallocatechin-3-Gallate (EGCG), as the major polyphenol that is found in green tea, and catechins and have shown considerable antioxidant capacity. Purpose Our study aims to explore the effects of EGCG on UVA-induced skin photoaging process and associated mechanisms. Methods In this study, human skin fibroblasts (HSFs) were treated with UVA and EGCG, and subsequent changes in cell morphology, telomeres, antioxidant capacity, cell cycle, and related genes were evaluated to examine the role and mechanisms of EGCG in delaying skin photoaging. Results HSF exposed to UVA underwent an increase in aging-related biomarkers and telomere shortening. Also, UVA radiation inhibited the secretion of transforming growth factor-beta1 (TGF-β1), induced cell cycle arrest, down-regulated antioxidant enzymes, and promoted the accumulation of oxidative product malondialdehyde (MDA) to cause further damage to cells. Increased expression of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinase-1 (TIMP-1), p66 at mRNA levels were also observed after UVA irradiation. EGCG treatment effectively inhibited above damage processes caused by UVA radiation in HSF. Conclusion Our study indicated that the potential mechanism of EGCG retarding photoaging is closely related to its powerful antioxidant effects and the ability to regulate the expression of related genes, and the usage of EGCG will be a potential strategy in preventing skin photoaging induced by UVA radiation.

Beverage consumption and facial skin aging: Evidence from Mendelian randomization analysis

“…Despite no statistical significance being observed in our MR analysis, some studies have reported tea intake is a protective agent for facial skin aging. 7,[46][47][48][49][50][51] Tea extracts have been found to improve wrinkles and attenuate light-induced skin aging in mouse models. 46,47 However, a randomized controlled trial in vivo found no statistically significant differences in skin aging after 2 years of supplementation with oral tea polyphenols.…”

“…7,47 The primary active ingredient of tea polyphenols, Epigallocatechin gallate (EGCG), has been identified as a potential anti-aging ingredient due to its function to scavenge ROS, inhibit the expression of MMPs, and increase collagen synthesis. [48][49][50][51] Studies have revealed inconsistent associations between alcohol intake and facial skin aging. [8][9][10][53][54][55] Some studies revealed that especially with heavy alcohol intake.…”

Extracellular vesicles derived from mesenchymal stem cells: the wine in Hebe's hands to treat skin aging

“…Jia et al . [ 48 ] observed significant telomere shortening in human skin fibroblasts following exposure to UVA radiation. Marion et al .…”

Photoprotective Effects of Epigallocatechin Gallate on Ultraviolet-Induced Zebrafish and Human Skin Fibroblasts Cells

“…Among these, EGCG is the major component of green tea polyphenols, which have been studied in many beneficial effects, such as anti-inflammatory, antioxidative, antimicrobial, and anticancer effects [ 9 ]. The study reported that EGCG could be a potential strategy in preventing human skin fibroblasts (HSF) photoaging induced by UVA radiation, is closely related to its powerful antioxidant effects to regulate the expression of related genes, like tissue inhibitor of metalloproteinase-1 (TIMP-1), p66 [ 10 ]. EGCG counters UVB-mediated phototoxicity via inhibition of the epidermal growth factor receptor in keratinocytes [ 11 , 12 ].…”